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Micro-capsulated suspended microbial seed coating agent and preparation method thereof

A technology of microcapsule suspension and microencapsulation, which is applied in the fields of botanical equipment and methods, chemicals for biological control, biocides, etc. problem, to achieve the effects of good slow release, good film formation, and strong application stability

Inactive Publication Date: 2015-03-25
SHIHEZI UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of the many advantages of the microcapsule dosage form, this dosage form is introduced into the new technology development idea of ​​microbial seed coating agents, so as to better solve the problems of low number of viable bacteria, poor application stability, and long-lasting effect of microbial seed coating agents. Problems such as short, high consumption and high cost
However, the retrieval of literature and published patents shows that the development technology of microencapsulated suspended microbial seed coating agents has not been reported at home and abroad at present.

Method used

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  • Micro-capsulated suspended microbial seed coating agent and preparation method thereof
  • Micro-capsulated suspended microbial seed coating agent and preparation method thereof
  • Micro-capsulated suspended microbial seed coating agent and preparation method thereof

Examples

Experimental program
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Effect test

preparation Embodiment 1

[0030] Bacillus amyloliquefaciens strain (DPPG-74 CGMCC No.9396) and Bacillus subtilis (DPPG-26 CGMCC No.9393) were mixed in a ratio of 2:1, and fermented to obtain a live bacteria concentration of 10 10-13 cfu / mL of fermentation broth for later use.

[0031] Bacterial microcapsules were prepared by endogenous emulsification method. Prepare 2% sodium alginate and 1% gelatin into solutions, mix them evenly, add 2% calcium carbonate particles, mix 25% of the fermented liquid of functional microorganisms with the composite wall material solution, and stir evenly to form a suspension; suspend the solution was added to 40% cottonseed oil (containing 0.5% Span80), emulsified under the action of magnetic stirring to form a water-in-oil emulsion; 5% glacial acetic acid was added to lower the pH value of the system, and the reaction was continued for 20 minutes, and 4% CaCl was added 2 Solution 50mL, such as figure 1 As shown, it can be observed that bacterial cell microcapsule balls...

preparation Embodiment 2

[0034] Mix Bacillus amyloliquefaciens (DPPG-74 CGMCC No.9396), Bacillus subtilis (DPPG-26 CGMCC No.9393) and Enterobacter cloacae (SRPG-70 CGMCC No.9395) in a ratio of 2:1:2, Fermentation was carried out to obtain a live bacteria concentration of 10 10-13 cfu / mL of fermentation broth for later use.

[0035] Preparation of microcapsules by endogenous emulsification method. Prepare 2% sodium alginate, 1.5% gelatin and 3% starch into solutions, mix well, add 2% calcium carbonate particles, mix 30% functional microbial fermentation broth with composite wall material solution, stir well, and form a suspension solution; add this suspension to 50% cottonseed oil (containing 0.5% Span80), and emulsify under the action of magnetic stirring to form a water-in-oil emulsion; add 5% glacial acetic acid to reduce the pH value of the system, and continue the reaction for 20 minutes , add 3% CaCl 2 The solution was 50 mL, and it could be observed that bacterial cell microcapsule balls were...

preparation Embodiment 3

[0038] Pseudomonas putida (SRPG-396 CGMCC No.9397) and Bacillus atrophaeus (DPPG-28 CGMCC No.9394) were mixed at a ratio of 1:1, and fermented to obtain a live bacteria concentration of 10 10-13 cfu / mL of fermentation broth for later use.

[0039] Preparation of microcapsules by endogenous emulsification method. Prepare 2% sodium alginate, 2% xanthan gum, and 3% starch into solutions, mix them evenly, add 2% calcium carbonate particles, mix 35% functional microbial fermentation broth with the composite wall material solution, and stir evenly. Form a suspension; join this suspension in 40% cottonseed oil (containing 0.5% Span80), carry out emulsification under the effect of magnetic stirring, form water-in-oil emulsion; Add 5% glacial acetic acid and reduce system pH value, continue React for 20min, add 4% CaCl 2 solution 50mL, it can be observed that bacterial cell microcapsule balls are formed in the solution, after standing, the oil phase is recovered and removed, and the ...

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Abstract

The invention relates to a micro-capsulated suspended microbial seed coating agent and a preparation method thereof. The micro-capsulated suspended microbial seed coating agent comprises a functional bacteria microcapsule and auxiliaries, wherein the functional bacteria microcapsule is prepared from wall materials such as sodium alginate and gelatin by virtue of an endogenous emulsion process, the grain size of the microcapsule is 20-60 mu m, the content of functional bacteria is not lower than 10<9>cfu / g, and the embedding rate is over 80%. Functional microbes are selected from two or more of pseudomonas, bacillus subtilis, bacillus amyloliquefaciens, enterobacter cloacae, raoultella planticola and bacillus atrophaeus. The auxiliaries comprise one or more of a film-forming agent, a dispersant, a tackifier, an anti-freezing agent and a coloring agent. The prepared functional bacteria microcapsule is uniformly mixed with the auxiliaries to obtain a finished product. The product has the advantages of good film-forming performances, thallus persistence, high survivability, good slow-controlled release, strong application stability, environment friendliness, convenience in use, and the like, can be used for promoting seed germination and seedling growth of cottons, soya beans, wheat and potatoes, and the like, controlling the diseases at the seedling stage, improving stress resistance, and the like.

Description

technical field [0001] The invention relates to a biological seed coating agent, in particular to a microencapsulated suspension microbial seed coating agent and a preparation method. The invention belongs to the technical field of agricultural biological preparation production. Background of the invention [0002] Biological seed coating agent is a seed coating agent made by fermentation and dosage form processing by using various bioactive ingredients produced or expressed by antagonistic microorganisms and their genes. Biological seed coating agent can improve the compatibility between seeds and soil, build a beneficial microbial environment, increase the germination rate of seeds, promote crop growth, and enhance stress resistance. The utility model has a wide application range and can be widely applied to various crop seeds. It decomposes quickly in nature, has no residue, no pollution, good safety, and has no negative impact on humans, animals or seeds. therefore. ...

Claims

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Application Information

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IPC IPC(8): A01N25/04A01N25/28A01N63/00A01P21/00
Inventor 武占省凃亮李春何艳慧杨红兵
Owner SHIHEZI UNIVERSITY
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