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Degrading bacteria capable of efficiently degrading pesticide chlorothalonil, and application thereof

A high-efficiency degrading bacteria and chlorothalonil technology, which is applied in the field of degrading bacteria, can solve the problem of reducing the degradation rate of pharmaceuticals, and achieve the effect of convenient cultivation and high-efficiency degradation ability

Inactive Publication Date: 2013-06-19
BEIJING UNIV OF AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although chlorothalonil degrades quickly in soil, the repeated use of the agent greatly reduces its degradation rate. Therefore, it is of great significance to screen microbial resources that can degrade chlorothalonil and enrich the database of chlorothalonil biodegradation

Method used

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  • Degrading bacteria capable of efficiently degrading pesticide chlorothalonil, and application thereof
  • Degrading bacteria capable of efficiently degrading pesticide chlorothalonil, and application thereof

Examples

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Effect test

specific Embodiment 1

[0023] Specific Example 1: Strain BJQ2 grows with chlorothalonil as the only carbon source and degrades chlorothalonil

[0024] Configure strain mother liquor (10 11 cfu mL -1 ), was inoculated into the basal medium containing chlorothalonil with an inoculum size of 10% by volume, and the final concentration of pesticide was 20mg L -1 , the filling volume is 50mL, the culture temperature is 30-35°C, the initial pH value is 7-8, and the rotation speed is 180r·min -1 , each treatment set up 3 parallels, with no inoculation as the control. After 2, 4, and 6 days of degradation culture, the content of chlorothalonil was detected by gas chromatography. The results showed that the residual amount of chlorothalonil in the medium gradually decreased after inoculation with BJQ2, and the residual amount was 3.17 mg·L after 6 days -1 , the degradation rate of chlorothalonil reached 79.2% (Table 1).

[0025] Table 1 Degradation rate of strain BJQ2 to chlorothalonil in inorganic salt ...

specific Embodiment 2

[0027] Specific example 2: Degradation of bacterial strain BJQ2 to chlorothalonil in soil

[0028] Pass the soil through a 0.2mm sieve, dry heat sterilization at 160°C for 1.5h, weigh 10g and place it in a 90mm petri dish, spray chlorothalonil mother solution, so that the initial concentration of chlorothalonil in the soil is 20mg L -1 , and evenly mix the BJQ2 bacterial solution cultivated for 36 hours into the soil so that the concentration is 10 7 / g soil, soil moisture 20%, set 3 repetitions, take the soil that adds medicine but does not add bacteria as control. The treated soil was incubated at room temperature, and samples were taken to detect the residual amount of chlorothalonil in the soil 1, 3, and 7 days after the treatment. At the same time, attention should be paid to spraying water the next day to keep the humidity at about 20%. The results showed that after the bacterial strain was cultured in soil for 7 days, the degradation rate of chlorothalonil could reach 9...

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Abstract

The invention relates to degrading bacteria capable of efficiently degrading pesticide chlorothalonil, and belongs to the field of biotechnology. A classification name of the degrading bacteria is Enterobacter cloacae B9; and a culture preservation number is CGMCCNo. 5527. Chlorothalonil is an organic chlorine farm-oriented broad spectrum bactericide and is proved to have very strong effects of carcinogenicity, teratogenicity and mutagenicity. Micro-biological degradation gains more and more significance because of rich bacteria resources in the environment without secondary pollution caused by introducing other chemical reagents. Microbe BJQ2 capable of degrading the chlorothalonil can be obtained by directional culture. The Microbe BJQ2 is identified as the Enterobacter cloacae B9 by morphology, physiological and biochemical analysis and 16SrDNA sequence homology alignment. The bacteria are the degrading bacteria by using the chlorothalonil as a sole carbon source. A degrading rate is 79.2% after being cultured for 6 days in an inorganic salt culture liquid; and a degrading rate is 92.3% after being used in a soil for 7 days. The degrading bacteria can be used for degradation of the bactericide chlorothalonil, and biological purification of water bodies, soil or agricultural products which are polluted by the chlorothalonil.

Description

technical field [0001] The invention belongs to the field of biological technology, and relates to a degrading bacterium capable of efficiently degrading the fungicide chlorothalonil and its application technical background [0002] Chlorothalonil (chlorothalonil) is an organochlorine agricultural broad-spectrum fungicide, which has a good control effect on downy mildew, powdery mildew, gray mold and other diseases, and is widely used in vegetables, fruit trees, beans, rice, crops such as wheat. In my country, the annual output exceeds 8,000 tons, and it is the second largest category of widely used agricultural fungicides. [0003] While it is widely used, it also brings corresponding toxic effects. Studies have shown that chlorothalonil can cause chronic toxicity problems. For example, chlorothalonil can induce sister chromatid exchange in mouse bone marrow lymphocytes and increase the micronucleus rate of bone marrow chromophilic erythrocytes. It is a chemical that muta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A62D3/02C02F3/34B09C1/10C12R1/01A62D101/04A62D101/26C02F101/38
Inventor 赵建庄王慧敏谷晓明魏朝俊贾临芳吴昆明梁丹
Owner BEIJING UNIV OF AGRI
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