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Bacillus radicicola capable of degrading pyridine as well as breeding method and application thereof

A rhizobacterium and pyridine technology, applied in the application field of wastewater biological treatment and environmental pollution remediation, can solve the problems of low treatment efficiency, low pyridine tolerance concentration, and single strain type, so as to reduce the workload and speed up the screening process , the effect of less bacteria

Active Publication Date: 2014-01-29
NANJING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a novel strain (Rhizobium sp.) NJUST18 that can efficiently degrade pyridine in view of the current pyridine-degrading strains that have a low tolerance to pyridine concentration, low treatment efficiency, and a relatively single type of strain.

Method used

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  • Bacillus radicicola capable of degrading pyridine as well as breeding method and application thereof
  • Bacillus radicicola capable of degrading pyridine as well as breeding method and application thereof
  • Bacillus radicicola capable of degrading pyridine as well as breeding method and application thereof

Examples

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Embodiment 1

[0023] Example 1: Screening and isolation of pyridine-degrading bacteria (Rhizobium sp.) NJUST18 and its degradation performance on pyridine

[0024] ⑴ Isolation of strains

[0025] Soil samples were excavated from a sewage outlet of a chemical company in Liuhe District, Nanjing, which had been polluted by pyridine for a long time, and 2g of soil samples were added to 50mL of liquid inorganic salt medium (MSM) with a pyridine concentration of 500mg / L, and put into a 150mL Erlenmeyer flask , under the condition of 30°C and 180 rev / min shaker culture to realize the enrichment culture of pyridine degrading bacteria. After 7 days, 2 mL of enriched culture medium was transferred into 50 mL of fresh medium, and cultured on a shaking table. After 3 consecutive transfers, the transferred liquid medium was diluted 10 with sterile distilled water. 5 -10 9 times, spread it on an inorganic salt solid medium plate with a pyridine concentration of 1000 mg / L and use pyridine as the only c...

Embodiment 2

[0039] Example 2: Effect of pyridine concentration on pyridine degradation performance in wastewater

[0040] Liquid MSM with pyridine concentrations of 600mg / L, 1000mg / L, 1600mg / L, 2000mg / L and 2600mg / L were prepared as simulated wastewater, and (Rhizobium sp.) NJUST18 seed solution was inserted into the simulated wastewater with a 5% inoculation amount . Depend on image 3 It was shown that (Rhizobium sp.) NJUST18 can completely degrade pyridine in simulated wastewater with pyridine concentration up to 2600mg / L. Under the conditions of pyridine concentrations of 600mg / L, 1000mg / L, 1600mg / L, 2000mg / L and 2600mg / L, the complete removal of pyridine in simulated wastewater can be achieved within 75h, 102h, 204h, 220h and 240h, respectively. With the degradation of pyridine, the bacterial concentration increased synchronously, and the yield coefficients were 0.51, 0.46, 0.33, 0.35 and 0.34, respectively. Due to the high toxicity and refractory properties of pyridine, under hig...

Embodiment 3

[0042] Embodiment 3: the influence of wastewater pH value on pyridine degradation performance

[0043] Prepare pyridine simulated wastewater with pyridine concentration of 1000 mg / L and initial pH of 5.0, 6.0, 7.0, 8.0, 9.0, 10.0, and inject (Rhizobium sp.) NJUST18 seed solution with 5% inoculation amount into simulated wastewater. Depend on Figure 4 The results show that (Rhizobium sp.) NJUST18 can degrade pyridine with high efficiency under the condition of initial pH of 7.0-8.0; under the condition of initial pH of 5.0-6.0, after a lag period of 72h, the degradation of pyridine is accelerated ; And under the condition of pH 9.0-10.0, pyridine degrades slowly, especially at the end of degradation.

[0044] This example shows that (Rhizobium sp.) NJUST18's optimal pH condition for degrading pyridine is neutral to slightly alkaline; in the range of pH 5.0-8.0, the pyridine degradation rate is relatively high; high pH conditions are not conducive to Degradation of pyridine. ...

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Abstract

The invention discloses bacillus radicicola capable of degrading pyridine as well as a breeding method and application thereof. The bacillus radicicola is bacillus radicicola (Rhizobiumsp.) by identification, and named (Rhizobiumsp.) NJUST18; the log-in number of GenBank is JN106368. The bacterial strain is preserved in the China center for type culture collection (CCTCC) on March 28th, 2013; the preservation number is CCTCC NO:M 2013110. Enrichment of pyridine degradation bacteria is carried out by directly adopting a culture medium taking pyridine as sole carbon source and nitrogen source; separation is carried out by adopting a screening culture medium taking the pyridine as the sole carbon source and nitrogen source; the screening process is quick and fast; sundry bacteria on the culture medium are few; the workload of affination is reduced. Compared with the other pyridine degradation bacteria, the bacterial strain has efficient pyridine degradation capability, good adaptive capacity and tolerance, and has a good application prospect in treatment of a high-concentration pyridine wastewater.

Description

technical field [0001] The invention belongs to the technical field of biological treatment of environmental organic pollutants, and in particular relates to a pyridine-degrading bacterium, a breeding method and its application in wastewater biological treatment and environmental pollution restoration. Background technique [0002] Pyridine is widely used in medicine, pesticide, chemical industry and other industries, and is a typical refractory nitrogen-containing heterocyclic compound. Pyridine has strong water solubility due to its heterocyclic structure, and is easily transferred to soil and groundwater, so it is ubiquitous in soil and groundwater. Due to the biotoxicity, mutagenesis and carcinogenic properties of pyridine, pyridine pollution has caused great potential harm to human health and ecological environment. Therefore, if the wastewater containing pyridine is discharged directly without treatment, it will cause serious pollution to the environment. [0003] At...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/02C02F3/34C12R1/41C02F101/38
Inventor 沈锦优王连军张鑫陈丹孙秀云李健生刘晓东
Owner NANJING UNIV OF SCI & TECH
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