Dominant consortium flora for degrading urban waste water and preparation method thereof

A technology for urban wastewater, dominant flora, applied in microorganism-based methods, biochemical equipment and methods, chemical instruments and methods, etc.

Inactive Publication Date: 2012-03-14
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the development of the economy, the composition of wastewater is becoming more and more complex, especially when the wastewater contains toxic and refractory organic pollutants, due to the small number and types of microorganisms with special degradation ability for this type of organic substances in the environment, and it At a disadvantage in interspecies competition, traditional biological treatment technologies face great challenges

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Pick three rings of bacteria respectively: Enterobacter cloacae, Gordonia, Pseudomonas putida (isolated from the sludge of urban sewage treatment plant) and separate them Transfer to a container containing 25 mL of nutrient solution, culture each type of bacteria at 30°C for 2 days, and then inoculate the three cultured bacteria into containers containing 500 mL of proliferation medium and culture at 30°C After 1-2 days, centrifuge at a speed of 6000rpm for 10min, and obtain the logarithmic growth phase cells of the above-mentioned three types of bacteria respectively. The logarithmic growth phase cells of the above three types of bacteria were taken out, washed 3 times with phosphate buffer saline, and 20% Enterobacter cloacae ( Enterobacter cloacae ), 54% Gordonella ( Gordonia ) and 26% Pseudomonas putida ( Pseudomonas putida ) were mixed, suspended in normal saline, and refrigerated for later use. Weigh calcium alginate and dissolve it in deionized water to make...

Embodiment 2

[0023] Pick three rings of bacteria respectively: Enterobacter cloacae ( Enterobacter cloacae ), Gordonella ( Gordonia ), Pseudomonas putida ( Pseudomonas putida ) (separated from the sludge of urban sewage treatment plant), they were transferred to containers containing 25 mL of nutrient solution, and each type of bacteria was cultured at 30°C for 2 days, and then the cultured three kinds of bacteria were separated Inoculate into a container containing 500mL of proliferation medium, culture at 30°C for 1-2 days, centrifuge at 6000rpm for 10min, and obtain the logarithmic growth phase cells of the above three bacteria respectively. The logarithmic growth phase cells of the above three types of bacteria were taken out, washed 3 times with phosphate buffer saline, and 24% Enterobacter cloacae ( Enterobacter cloacae ), 58% Gordonella ( Gordonia ) and 18% Pseudomonas putida ( Pseudomonas putida ) were mixed, suspended in normal saline, and refrigerated for later use. Weig...

Embodiment 3

[0026] Pick three rings of bacteria respectively: Enterobacter cloacae ( Enterobacter cloacae ), Gordonella ( Gordonia ), Pseudomonas putida ( Pseudomonas putida ) (separated from the sludge of urban sewage treatment plants) were transferred to containers containing 25 mL of nutrient solution, and each type of bacteria was cultured at 30°C for 2 days, and then the cultured three kinds of bacteria were inoculated separately Cultivate in a container containing 500mL of proliferation medium at 30°C for 1-2 days, centrifuge at 6000rpm for 10min, and obtain the logarithmic growth phase cells of the above three bacteria respectively.

[0027] The logarithmic growth phase cells of the above three types of bacteria were taken out, washed 3 times with phosphate buffer saline, and 22% Enterobacter cloacae ( Enterobacter cloacae ), 56% Gordonella ( Gordonia ) and 22% Pseudomonas putida ( Pseudomonas putida ) were mixed, suspended in normal saline, and refrigerated for later use. ...

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Abstract

The invention discloses a dominant consortium flora for degrading urban waste water and a preparation method thereof. The preparation method comprises the following steps of: inoculating and culturing enterobactercloacae, gordonia and pseudomonasputida respectively; mixing 20-24 percent by volume of enterobactercloacae, 54-58 percent by volume of gordonia and 26-18 percent by volume of pseudomonasputida respectively; and preparing calcium alginate immobilized particles, activating, and directly putting into urban waste water. By coordinating among strains, the TOC (Total Organic Carbon) degrading efficiency of urban waste water by microbial treatment can be increased effectively.

Description

technical field [0001] The invention relates to the field of urban wastewater treatment, in particular to a dominant bacterial group for degrading urban wastewater and a preparation method thereof. Background technique [0002] Biological treatment is a commonly used wastewater treatment method at present. This method decomposes and absorbs the pollutants in wastewater through the metabolism of microorganisms, so as to achieve the purpose of pollution control. Compared with other methods, biological treatment has low cost, high efficiency, easy operation, and most importantly, no secondary pollution. Therefore, it has been widely used in the treatment of urban sewage and industrial sewage. With the development of the economy, the composition of wastewater is becoming more and more complex, especially when the wastewater contains toxic and refractory organic pollutants, due to the small number and types of microorganisms with special degradation ability for this type of organ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/10C12N11/04C02F3/34C12R1/01C12R1/40
Inventor 陈元彩孙友友
Owner SOUTH CHINA UNIV OF TECH
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