Preparation method of paecilomyces lilacinus biological fertilizer
A technology of Paecilomyces lilacinus and biological fertilizer, which is applied in the field of preparation of Paecilomyces lilacinus biological fertilizers, can solve the problems of environmental pollution, poor medicinal and fertilizer effects, etc., and achieves the promotion of plant growth and significant economic benefits. Benefit and environmental protection benefit, the effect of high sporulation rate
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Embodiment 1
[0033] (1) Preparation of the secondary liquid strain of Paecilomyces lilacinus, the specific operation steps are as follows:
[0034] A. Inclined strain (solid)
[0035] Weigh 20 parts of potatoes, wash, peel and cut into small pieces, add 100 parts of water and boil for half an hour or high-pressure steaming for 20 minutes, filter with gauze, add 2 parts glucose and 2 parts agar, fully dissolve and filter while hot gauze. Load the test tube, sterilize with 15 pounds of steam (121°C) for 20 minutes, take out the test tube and place it on a slope. After cooling, inoculate with Paecilomyces lilacinus NH-PL-03 strain, and place it in a thermostat at 28°C for 8 days.
[0036] B. Secondary strain (liquid)
[0037] The medium formula is the same as that of the inclined plane but no agar is added. It is divided into Erlenmeyer flasks. After sterilization and cooling, on the aseptic operation table, use an inoculation loop to extract conidia from Paecilomyces lilacinus preserved in the solid...
Embodiment 2
[0045] Example 2: Preparation of fertilizer medicine of Paecilomyces lilacinus
[0046] The steps different from Embodiment 1 are as follows, and the other unexplained steps are the same as Embodiment 1:
[0047] Step (2) Preparation of solid fermentation culture of Paecilomyces lilacinus by thick-layer aeration fermentation
[0048] 70 parts of fermented mattress material for decomposed farms, 15 parts of rice bran, 15 parts of corn starch, stir evenly, add water to adjust the moisture content to 40%, and inoculate 8 parts of liquid strain for production in step (1) (the above are all by weight Part), transferred to a solid fermentation tank, ventilated, fermented at 27°C, turned once for 24 to 30 hours, and fermented for 9 days to obtain a solid fermentation culture of Paecilomyces lilacinus.
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