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Preparation method of paecilomyces lilacinus biological fertilizer

A technology of Paecilomyces lilacinus and biological fertilizer, which is applied in the field of preparation of Paecilomyces lilacinus biological fertilizers, can solve the problems of environmental pollution, poor medicinal and fertilizer effects, etc., and achieves the promotion of plant growth and significant economic benefits. Benefit and environmental protection benefit, the effect of high sporulation rate

Inactive Publication Date: 2010-03-17
福建省农业科学院农业生物资源研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to provide a kind of preparation method of Paecilomyces lilacinus biofertilizer, solve the problems in the prior art such as simply mixing medicine and fertilizer, poor medicinal and fertilizer effects, polluting the environment, etc. series of problems, the preparation process is simple and easy, and the bacterial activity in the cultivated Paecilomyces lilacinus biological fertilizer is good. When solid fermentation is cultivated, the fertilizer is directly used as the culture medium, and microorganisms are used as medicinal components, so that the fertilizer of the present invention has The organic combination of medicine and fertilizer, the fertilizer and drug effects are very significant, and it has no pollution to the environment, and has significant economic benefits and environmental protection benefits

Method used

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  • Preparation method of paecilomyces lilacinus biological fertilizer
  • Preparation method of paecilomyces lilacinus biological fertilizer
  • Preparation method of paecilomyces lilacinus biological fertilizer

Examples

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Embodiment 1

[0033] (1) Preparation of the secondary liquid strain of Paecilomyces lilacinus, the specific operation steps are as follows:

[0034] A. Inclined strain (solid)

[0035] Weigh 20 parts of potatoes, wash, peel and cut into small pieces, add 100 parts of water and boil for half an hour or high-pressure steaming for 20 minutes, filter with gauze, add 2 parts glucose and 2 parts agar, fully dissolve and filter while hot gauze. Load the test tube, sterilize with 15 pounds of steam (121°C) for 20 minutes, take out the test tube and place it on a slope. After cooling, inoculate with Paecilomyces lilacinus NH-PL-03 strain, and place it in a thermostat at 28°C for 8 days.

[0036] B. Secondary strain (liquid)

[0037] The medium formula is the same as that of the inclined plane but no agar is added. It is divided into Erlenmeyer flasks. After sterilization and cooling, on the aseptic operation table, use an inoculation loop to extract conidia from Paecilomyces lilacinus preserved in the solid...

Embodiment 2

[0045] Example 2: Preparation of fertilizer medicine of Paecilomyces lilacinus

[0046] The steps different from Embodiment 1 are as follows, and the other unexplained steps are the same as Embodiment 1:

[0047] Step (2) Preparation of solid fermentation culture of Paecilomyces lilacinus by thick-layer aeration fermentation

[0048] 70 parts of fermented mattress material for decomposed farms, 15 parts of rice bran, 15 parts of corn starch, stir evenly, add water to adjust the moisture content to 40%, and inoculate 8 parts of liquid strain for production in step (1) (the above are all by weight Part), transferred to a solid fermentation tank, ventilated, fermented at 27°C, turned once for 24 to 30 hours, and fermented for 9 days to obtain a solid fermentation culture of Paecilomyces lilacinus.

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Abstract

The invention provides a preparation method of paecilomyces lilacinus biological fertilizer, belonging to the technical field of agrobiomechanics, and solving the problems in the prior art. The preparation method comprises the steps of: taking the paecilomyces lilacinus as bacterial strain, inoculating triangular flask liquid bacterial strain, and fermenting with czapek-dox medium to prepare liquid bacterial strain for production; taking decomposed or semi-decomposed fermenting bed padding materials in a culturing farm as basic medium, matching with accessorial carbon source and water, inoculating the liquid bacterial strain, and fermenting in a ventilation way in the depth layer of a solid fermenting pond to prepare paecilomyces lilacinus solid fermentation culture material; and drying the paecilomyces lilacinus solid fermentation culture material and grinding. The invention takes high-effective eelworm biocontrol bacteria paecilomyces lilacinus as the bacterial strain, takes the decomposed or semi-decomposed fermenting bed padding materials in the culturing farm as main raw materials, and ferments to prepare the paecilomyces lilacinus biological fertilizer to be taken as soil microorganism fertilizer which can prevent and cure the parasitic eelworm and various plant pathogenic bacteria, promote the plant growth, and reduce the pesticide residue in the soil.

Description

Technical field [0001] The invention relates to a soil microbial fertilizer medicine for both fertilizer and medicine, and more particularly relates to a preparation method of Paecilomyces lilacinus biological fertilizer medicine. Background technique [0002] In traditional agricultural production, in order to increase production and income, farmers often use a large number of chemical fertilizers and production hormones to promote crop growth, and use pesticides to control pests and diseases. However, these fertilizers, pesticides and production hormones are used almost at the same time. Auxiliary additives such as toluene have remained unbalanced, degraded and altered in soil structure, land fertility and plant ecology in many places, resulting in persistent diseases of crops, especially vegetables and fruit trees. Chemical pesticides used to treat these diseases It also increased the pollution of land and agricultural products. Under such circumstances, some manufacturers ha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00C05G3/02C05F17/00C12N1/14C12R1/79A01N63/04A01P5/00A01P3/00C05G3/60
CPCY02W30/40
Inventor 刘波史怀
Owner 福建省农业科学院农业生物资源研究所
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