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Production process for fermenting biocontrol trichoderma by using Chinese herbal medicine dregs

A biocontrol technology of Trichoderma and Chinese herbal medicine, applied in the field of agricultural biology, can solve the problems of land occupation and environmental pollution.

Inactive Publication Date: 2010-06-23
텐진인스티튜트오브플랜트프로텍션
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditionally, landfill and other treatment methods are often used, which neither fundamentally solves the problem of environmental pollution, but also occupies land and costs a lot of money.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach 1

[0014] Embodiment 1. The invention discloses a method for fermenting and producing Trichoderma viride using traditional Chinese medicine dregs as a raw material. It includes PDA activation of strains, seed liquid fermentation culture and solid fermentation culture of Chinese herbal medicine dregs, Chinese herbal medicine dregs, corn flour, and bean cake powder are mixed in a ratio of 90:8:2, and then water is added to make the water content reach 65% to 75%. . Traditional Chinese medicine residue can choose any of the following formulas: 1. Huoxiang medicinal residue; 2. Panax notoginseng medicinal residue; 3. Licorice medicinal residue; 4. Shouwu medicinal residue; 5. Yinqiao medicinal residue; 7. Mulberry leaf dregs; 8. Magnolia officinalis dregs; 9. Scutellaria baicalensis dregs; 9. Gynostemma pentaphylla; 10. Forsythia dregs. Under the condition of 0.13~0.17MPa high pressure and 121℃, sterilize for 85~120min. According to the ratio of seed liquid: solid base material = ...

Embodiment approach 2

[0015] Embodiment 2. The invention discloses a method for fermenting and producing Trichoderma viride using traditional Chinese medicine dregs as a raw material. It includes PDA activation of strains, seed liquid fermentation culture and solid fermentation culture of Chinese herbal medicine dregs, Chinese herbal medicine dregs, corn flour, and bean cake powder are mixed in a ratio of 90:8:2, and then water is added to make the water content reach 65% to 75%. . The residue of traditional Chinese medicine can be selected from any of the following formulas: 1. Dregs of Huoxiang Zhengqi dripping pills; 2. Dregs of compound Danshen dripping pills; 3. Dregs of compound licorice tablets; 4. Dregs of compound Yinqiao tablets; 6. Dregs of Qishen Yiqi Dropping Pills; 7. Dregs of Bupleurum Dropping Pills. Under the condition of 0.13~0.17MPa high pressure and 121℃, sterilize for 85~120min. According to the ratio of seed liquid: solid base material = 2.5 ~ 10: 100, the seed liquid is ev...

Embodiment approach 3

[0016] Embodiment 3. The invention discloses a method for fermenting and producing Trichoderma harzianum from traditional Chinese medicine dregs. It includes PDA activation of strains, seed liquid fermentation culture and solid fermentation culture of Chinese herbal medicine dregs, Chinese herbal medicine dregs, corn flour, and bean cake powder are mixed in a ratio of 91:7:2, and then water is added to make the water content reach 65% to 75%. . The residue of traditional Chinese medicine can be selected from any of the following formulas: 1. Dregs of Huoxiang Zhengqi dripping pills; 2. Dregs of compound Danshen dripping pills; 3. Dregs of compound licorice tablets; 4. Dregs of compound Yinqiao tablets; 6. Dregs of Qishen Yiqi Dropping Pills; 7. Dregs of Bupleurum Dropping Pills. The batch sterilization method was adopted for 3 consecutive days, steam sterilization at 100°C for 30-60 minutes every day. According to the ratio of seed liquid: solid base material = 2.5 ~ 10: 10...

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Abstract

The invention relates to a method for fermenting biocontrol trichoderma spores by using Chinese herbal medicine dregs as main culture substrate materials, belonging to the field of agricultural biotechnology. The biocontrol trichoderma has obvious effect on controlling various plant diseases, and the development of the production process for improving spore yield, shortening fermentation time and reducing fermentation cost is favorable for preparing the biocontrol trichoderma into preparations. The technical essential of the invention is to use the Chinese herbal medicine dregs as main raw materials to manually ferment and culture biocontrol trichoderma such as trichoderma viride, trichoderma harzimum and the like, and the production process comprises the steps of strain culture, preparation of fermentation substrates, inoculation, spore culture and the like. The invention is mainly characterized in that the raw materials used as culture substrate materials are obtained by selecting waste Chinese herbal medicine dregs through a proper formula after medicinal components are extracted in the process of preparing Chinese traditional medicines in a Chinese traditional medicine factory to be used for fermenting and culturing the biocontrol trichoderma. The method has simple process, easy acquisition of raw materials and low cost. The biocontrol trichoderma spore microbial inoculum produced by the method has better storage resistance and biocontrol activity.

Description

technical field [0001] The invention relates to a method for using Chinese herbal medicine dregs to ferment and control Trichoderma, belonging to the field of agricultural biotechnology. Background technique [0002] Trichoderma is a type of fungus that is ubiquitous in nature, and can often be isolated in soil, plant root circumference, leaf circumference, seeds, and bulb surfaces. In recent years, it has been found that some Trichoderma are parasites or antagonists of plant pathogenic fungi, such as Trichoderma harzianum, T.hamatum, and T.viride, which can control fungi disease. So far, the research on Trichoderma in biological control has been carried out for nearly 70 years, and commercial Trichoderma preparations have appeared abroad. For example, Trichoderma preparations for the prevention and treatment of Prunus fruit tree silver leaf disease (Chondrostereum purpureum) have been commercially produced in Western Europe; Israel's Makhteshim Agan company has developed ...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/885
Inventor 王勇王万立赵阵雨刘春艳郝永娟刘晓琳霍建飞
Owner 텐진인스티튜트오브플랜트프로텍션
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