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Liquid composite microorganism fertilizer and its preparation method

A technology of compound microorganisms and fertilizers, applied in the field of new liquid compound microbial fertilizers and its preparation, to achieve the effects of promoting plant growth, enhancing stress resistance and disease resistance, and high content of probiotics

Active Publication Date: 2007-02-14
山东省农业科学院高新技术研究中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Preparation of liquid compound microbial fertilizer by microbial fermentation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1: the preparation of the secondary liquid bacterial classification of brewer's yeast, concrete operating steps are as follows:

[0018] A. Slope bacteria (solid)

[0019] Peel and wash the potatoes, cut them into small pieces, put them in 96.5 parts of water, potatoes: water = 1: (3-5) weight ratio, boil for 30 minutes, filter with filter cloth, dilute the filtrate with water to the original volume. Add 2 parts of glucose, 1.5 parts of agar, the natural pH value, divide into test tubes, and sterilize at 15 pounds for 20 minutes. Streak inoculation of brewer's yeast strain and culture at 28-32°C. All the above are parts by weight.

[0020] B. Secondary strains (liquid)

[0021] The formula of the culture medium is the same as that of the slant, but without adding agar, the natural pH value is divided into 500ml Erlenmeyer flasks and stuffed with cotton plugs, sterilized at 15 pounds for 20 minutes, and inoculated with 3% to 5% by weight of the bacterial su...

Embodiment 2

[0022] Embodiment 2: the preparation of the secondary liquid strain of Lactobacillus plantarum and Lactobacillus acidophilus, the specific operation steps are as follows:

[0023] A. Solid bacteria

[0024] 1 part of peptone, 0.5 parts of yeast powder, 0.2 parts of ammonium citrate, 2 parts of glucose, 0.054 parts of magnesium sulfate, 1 part of beef extract, 0.2 parts of dipotassium hydrogen phosphate, 0.5 parts of sodium acetate, 0.025 parts of manganese sulfate, 1.5 parts of agar, 93.021 parts of water, natural pH value, divided into test tubes, sterilized at 15 pounds for 20 minutes. Streak inoculation of Lactobacillus plantarum and Lactobacillus acidophilus respectively, and culture at 30-37°C. All the above are parts by weight.

[0025] B. Secondary strains (liquid)

[0026] The formula of the culture medium is the same as that of the solid culture medium, but no agar is added, the natural pH value is divided into 500ml Erlenmeyer flasks with rubber stoppers, steriliz...

Embodiment 3

[0027] Embodiment 3: the preparation of liquid compound microbial fertilizer

[0028] Preparation method: Mix 5 parts of potatoes, 5 parts of brown sugar, 2 parts of glucose, 0.5 parts of peptone, 0.1 part of magnesium sulfate, 0.1 part of manganese sulfate, 0.1 part of dipotassium hydrogen phosphate, and 0.1 part of sodium chloride, and add 87.1 parts of clean water Dissolve, sterilize with steam at 116°C, first inoculate 1 portion of beer yeast secondary liquid strain, cultivate at 30-32°C for 16 hours, then inoculate two probiotics, Lactobacillus plantarum and Lactobacillus acidophilus, in a volume ratio of 1:1 Mix 2 parts of secondary liquid strains (seed liquid) in total, continue to cultivate at 36-37°C for 26 hours, harvest when the pH drops to 3.5, and bottle it under aseptic conditions. The obtained compound microbial fertilizer is an acidic brown liquid with a live bacteria content of 10 billion / mL. All the above are parts by weight.

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Abstract

The process of preparing liquid compound microbial fertilizer belongs to the field of agricultural biotechnology. The preparation process includes inoculating beer yeast, plant lactobacillus and Bacillus lacticus separately to produce liquid bacteria seeds in conical flasks; mixing potato, brown sugar, glucose, peptone, magnesium sulfate, manganese sulfate, dipotassium hydrogen phosphate and sodium chloride in certain weight proportion; dissolving the mixture and heat treatment to sterilize; inoculating beer yeast, plant lactobacillus and Bacillus lacticus successively; gathering after pH value is lowered to 3.5-4.5; and abacterial packing. The liquid compound microbial fertilizer is acid brown liquid with live bacteria content over 1 billion / ml, and may be used as microbial soil improver and as foliage fertilizer to promote plant growth and raise plant resistance.

Description

(1) Technical field [0001] The invention relates to a novel liquid compound microbial fertilizer and a preparation method thereof, belonging to the field of agricultural biotechnology. (2) Background technology [0002] Among the many measures to ensure agricultural production increase, the application of chemical fertilizers plays an important and irreplaceable role. Extensive use of chemical fertilizers increases the yield of crops, but also causes a large accumulation of nitrate and nitrite in the soil, increases the residual amount of harmful chemical substances in agricultural products, reduces the quality and safety of agricultural products. At the same time, the loss of nitrate and nitrite accumulated in the soil pollutes water sources and causes great damage to the ecological environment. At present, the amount of chemical fertilizer per unit area in my country is already in the forefront of the world, and there is still a growing trend. Therefore, seeking to use h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G1/00C05F11/08C12N1/20C12N1/16
Inventor 岳寿松尤升波游银伟王翠萍李爱芹
Owner 山东省农业科学院高新技术研究中心
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