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Soil anaerobic disinfection method for preventing and controlling tomato bacterial wilt

A technology of tomato bacterial wilt and disinfection method, which is applied in the field of soil anaerobic disinfection method for preventing and controlling tomato bacterial wilt, can solve the problems of environmental damage and human health, damage, etc., and achieve the reduction of the number of bacterial wilt, good control effect, Growth-promoting effect

Inactive Publication Date: 2017-03-29
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But because methyl bromide is a substance that damages the ozone layer, it seriously damages the environment and human health

Method used

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  • Soil anaerobic disinfection method for preventing and controlling tomato bacterial wilt
  • Soil anaerobic disinfection method for preventing and controlling tomato bacterial wilt
  • Soil anaerobic disinfection method for preventing and controlling tomato bacterial wilt

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] 1. Test material

[0036] (1) The test soil was collected from a tomato and rice rotation planting field in Zhucun Town, Zengcheng District, Guangzhou City, Guangdong Province (113.70° east longitude; 23.28° north latitude). This field has a history of tomato bacterial wilt and is slightly acidic. Properties: organic matter content 13.7g kg -1 , total nitrogen content 0.82g·kg -1 , total P content 1.65g·kg -1 , total K content 28.1g·kg -1 , pH=4.66.

[0037] (2) The organic material is rice bran (rice husk), and the diameter of the rice bran is less than 0.2cm.

[0038] (3) The strain of R. solanacearum (R. solanacearum) was selected from physiological race I and biochemical type III, provided by the College of Horticulture, South China Agricultural University.

[0039] 2. Test method

[0040] (1) Preparation of bacterial suspension of R. solanacearum:

[0041] Place R. solanacearum strains stored at room temperature in 2,3,5-triphenyltetrazolium chloride medium,...

Embodiment 2

[0062] 1, carry out embodiment 2 on the basis of embodiment 1, adopt the same test material as embodiment 1, comprise the following steps:

[0063] (1) There are 4 treatments in the experiment, namely:

[0064] Control group (CK);

[0065] Add 2% w / w rice bran at 25°C + water irrigation and cover film to seal anaerobic treatment (ADB25);

[0066] Add 2% w / w rice bran at 30°C + water irrigation and cover film to seal anaerobic treatment (ADB30);

[0067] Add 2% w / w rice bran at 35°C + irrigate and cover film to seal anaerobic treatment (ADB35);

[0068] Each treatment was repeated 4 times.

[0069] (2) Each experimental group weighed 2.0 kg of soil for testing, manually added 20 mL of bacterial suspension, and added 10 mL of bacterial suspension per kg of soil; according to the experimental design, except for the control, the other 3 experimental groups were added with 40.0 g of rice bran, Mix well, transfer to 20×30cm black ziplock bag, place in day / night temperature of 25...

Embodiment 3

[0078] The optimization of embodiment 3 rice bran addition

[0079] 1. The experimental materials are the same as in Example 1.

[0080] 2. Experimental method

[0081] (1) There are 4 treatments in the experiment, namely:

[0082] Control group (CK1);

[0083] Moisturizing treatment (CK2);

[0084] Addition of 2% w / w rice bran + sealed anaerobic treatment (2% rice bran);

[0085] Addition of 4% w / w rice bran + sealed anaerobic treatment (4% rice bran);

[0086] Each treatment was repeated 4 times.

[0087] (2) Each experimental group weighed 2.0kg of soil for testing, manually added 20mL of bacterial suspension, and added 10mL of bacterial suspension per kg of soil; according to the experimental design, except for CK1 and CK2, the other two experimental groups added 40.0g and 80.0g rice bran, mixed well, transferred to 20×30cm black ziplock bags, in which CK2 was kept moist, 2% rice bran and 4% rice bran treatment groups were irrigated to the field water capacity and se...

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Abstract

The invention discloses a soil anaerobic disinfection method for preventing and controlling tomato bacterial wilt. The method specifically comprises the steps of adding 2-4% by mass of rice bran, wheat bran, tea seed bran or peanut bran to soil containing ralstoniasolanacearum comb.nov; performing full uniform mixing; performing anaerobic treatment for 3-5 weeks in a waterflooding way or in a way of irrigating water to field capacity and covering an airtight membrane; and removing the membrane and keeping the idle state for a few days, so that plant seedlings can be transplanted. The soil treated with the method has good prevention and control effects for the tomato bacterial wilt; the prevention effect of a tray-based planting experiment is 100%; the plant height, stem thickness and fresh weight of a tomato can be remarkably increased; and the tomato growth can be greatly facilitated. The method has the advantages that the tomato bacterial wilt is controlled from the soil source, the prevention and control effects are good, no negative influence is caused to the environment, and the like; and the method has relatively high application potential.

Description

technical field [0001] The invention belongs to the technical field of plant disease prevention and control, and in particular relates to a soil anaerobic disinfection method for preventing and treating tomato bacterial wilt. Background technique [0002] With the continuous promotion and development of high-input, high-yield crop intensive planting methods, the continuous cropping obstacles mainly caused by soil-borne diseases and soil degradation have become increasingly prominent, which has brought huge economic losses to crop production and seriously restricted my country's agricultural production development. Ralstonia solanacearum comb.nov is a devastating soil-borne disease caused by Ralstonia solanacearum comb.nov, known as "plant cancer", which seriously restricts the sustainable development of vegetable production. Bacterial wilt is particularly harmful to solanaceous vegetable crops (tomato, eggplant, pepper, etc.). Because bacterial wilt is a soil-borne disease,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L2/02A01C21/00
CPCA61L2/02A01C21/00
Inventor 蔡昆争伍朝荣黄飞
Owner SOUTH CHINA AGRI UNIV
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