Rhizospheric prebiotics composition for reducing incidence rate of tomato bacterial wilt and application of composition

A prebiotic and composition technology, applied in the field of rhizosphere prebiotic composition, can solve problems such as failure to achieve effects, and achieve the effects of reduced investment, easy availability of raw materials, and better comprehensive prevention and control effects.

Pending Publication Date: 2020-01-14
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Various control measures have their own advantages and disadvantages, but they still cannot achieve satisfactory results in actual agricultural production.

Method used

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  • Rhizospheric prebiotics composition for reducing incidence rate of tomato bacterial wilt and application of composition
  • Rhizospheric prebiotics composition for reducing incidence rate of tomato bacterial wilt and application of composition
  • Rhizospheric prebiotics composition for reducing incidence rate of tomato bacterial wilt and application of composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1. Obtaining of prebiotic formula

[0027] The rhizosphere soil samples of healthy and bacterial wilt diseased tomato were collected from the tomato experimental field of Nanjing Institute of Vegetables, and the rhizosphere metabolome of healthy and diseased tomato was determined by gas chromatography-mass spectrometry (GC-MS). The results showed that there were significant differences in metabolites in the rhizosphere of healthy and diseased tomatoes, and the substances significantly enriched in the rhizosphere of healthy tomatoes included melibiose, fructose, sucrose, ribose, xylose, mannose, maltose, gluconolactone, Inositol, Adonitol, Isoleucine, Alanine, Aspartic Acid, Tryptophan. Subdivision found that these 14 substances belong to 4 categories, namely sugars (melibiose, fructose, sucrose, ribose, xylose, mannose, maltose), esters (gluconolactone), alcohols (muscleose alcohol, adonitol), amides (isoleucine, alanine, aspartic acid, tryptophan). Therefo...

Embodiment 2

[0029] Example 2. Further optimization of prebiotic formula

[0030] With reference to the experimental method of Example 1, further studies have found that when the combination of prebiotics contains more than 7 (including 7) substances, its effect is not significantly different from that of all 10 substances, such as figure 2 Shown are the inhibitory effects of three preferred formulations comprising more than 7 substances (see Table 1 for formulation) and all 10 formulations of substances on R. solanacearum in soil.

[0031] Table 1 Three preferred prebiotic formulations

[0032] formula Substance type 1 Melibiose, fructose, ribose, xylose, mannose, maltose, inositol 2 Fructose, Sucrose, Ribose, Xylose, Mannose, Maltose, Adonitol 3 Melibiose, fructose, sucrose, ribose, xylose, mannose, maltose

Embodiment 3

[0033] Example 3. The application of rhizosphere prebiotics can help tomato resist the invasion of pathogenic bacteria

[0034] 3.1 Applying rhizosphere prebiotics to reduce the incidence of tomato bacterial wilt

[0035] The tomato seedlings that have been cultivated for about one month are transplanted into 32 hole hole trays (the depth of the hole in each hole is 5cm, the diameter is 6cm, and the bottom is 3cm), and 50g of soil containing Ralstonia solanacearum is added to each hole (Ralstonia solanacearum bacterium liquid The concentration is 5*10 7 CFU / g). One week after transplanting, an aqueous solution of prebiotics was added to the rhizosphere of each tomato hole, so that the final concentration of prebiotics was 50 μmol / kg, and the experimental control was the treatment of adding the same amount of sterile water. Add once a week, and after cultivating for one month, the incidence of tomato disease is counted. Such as image 3 As shown, the incidence of bacterial ...

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Abstract

The invention discloses a rhizospheric prebiotics composition for reducing the incidence rate of tomato bacterial wilt and an application of the composition. The soil microbial prebiotics compositioncomprises a carbohydrate substance or a carbohydrate substance, an ester substance and an alcohol substance, the carbohydrate substance is selected from any one or more of melibiose, fructose, sucrose, ribose, xylose, mannose and maltose, the ester substance is selected from gluconolactone, and the alcohol substance is selected from any one or two of inositol and ribtol adonite. The prebiotics composition is applied, total bacteria abundance in rhizospheric soil can be improved, proliferation of ralstonia solanaceara is restrained, so that the incidence rate of bacterial wilt is remarkably reduced, and the rhizospheric prebiotics composition is suitable for being applied to crops easily subjected to the bacterial wilt.

Description

technical field [0001] The invention belongs to the technical field of agricultural chemicals, and relates to a rhizosphere prebiotic composition for reducing the incidence of tomato bacterial wilt and its application. Background technique [0002] Tomato bacterial wilt is a common soil-borne bacterial disease. It is easy to occur in continuous cropping fields under high temperature and high humidity conditions. In the early stage of the disease, the aboveground part of the plant will show symptoms of wilting, but the leaves will remain green. As the disease spreads , it will cause large areas of wilting of tomato plants and a sharp drop in tomato yield. The pathogen of tomato bacterial wilt is Ralstonia solanacearum. Ralstonia solanacearum can invade the xylem vessel of the plant through the plant root system and proliferate in large numbers, resulting in plant death. The host range of R. solanaceae is very wide, and it can infect more than 200 kinds of monocotyledonous an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G22/05A01G7/06A01N43/16A01N43/08A01N31/02A01N31/06A01P1/00
CPCA01G7/06A01N31/02A01N31/06A01N43/08A01N43/16A01G22/05
Inventor 袁军沈其荣刘婷文涛
Owner NANJING AGRICULTURAL UNIVERSITY
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