70results about How to "Reduce reproductive costs" patented technology

The invention discloses an in vivo reproduction method of entomopathogenic nematodes, relating to an in vivo culture method of entomopathogenic nematodes and aiming to solve the problems of low yield in applying the tenebrio molitors to breed the nematodes. The in vivo reproduction method comprises the following steps of: firstly, cutting two pieces of medium speed qualitative filter paper and filling in a cover of a culture dish A; then placing prodenia litura larvae; then soaking the medium speed qualitative filter paper by using water suspension infective juveniles; covering the bottom of the culture dish A to the cover of the culture dish A till all prodenia litura larvae die; removing the bottom of the culture dish A; placing the cover of the culture dish A in a culture dish B; filling sterile water to a gap between the culture dish B and the culture dish A; culturing at a constant temperature till the infective juveniles occur in the sterile water; then collecting the sterile water with the infective juveniles every day; and complementing the sterile water so as to complete the in vivo reproduction of the entomopathogenic nematodes. The in vivo reproduction method of the entomopathogenic nematodes is mainly used for the in vivo reproduction of the entomopathogenic nematodes.

The invention relates to a method for breeding Scleroderma guani Xiao et Wu in a non-induced way. The method comprises the steps of female warp obtaining, storage, multiplying, rejuvenation, propagation expanding, quality inspection, packaging, storage, transportation and the like. The method for breeding the Scleroderma guani Xiao et Wu in a non-induced way, provided by the invention, is mainly used for multiplying the Scleroderma guani Xiao et Wu by using barley pests as alternative hosts.

The invention provides an efficient breeding method of purple potato tissue culture seedlings. The efficient breeding method of the purple potato tissue culture seedlings comprises the following steps of treating purple potato tubers at ultralow temperature; performing induced culture on root tips and stem tips of seed potatoes; performing rooting culture on strong seedlings, and conducting virus detection on test-tube plantlets; performing induced culture and virus detection on miniature potatoes; performing transplanting and cuttage grafting breeding on the miniature potatoes. According to the efficient breeding method of the purple potato tissue culture seedlings, provided by the invention, miniature potato seedlings are bred by adopting virus eradication of stem tips and root tips; the culture cycle of the root tips is different from that of the stem tips; the root tips and the stem tips are jointly cultured, so that the time cost can be effectively reduced, seed potato resources are fully and efficiently utilized, and further the reproduction cost of the tissue culture seedlings is reduced; besides, the test-tube plantlets cultured by using the root tips are high in reproduction speed, so that the working efficiency is improved, and the production cost is reduced.

The invention provides a plumbago auriculata shoot cutting propagation method. The method comprises the following steps: (1) selecting a cutting substrate; (2) selecting a cutting shoot; (3) treating by a rooting agent; (4) performing the cutting; (5) managing after the cutting. Compared with the prior art, the plumbago auriculata shoot cutting propagation method not only has the advantages of the prior art such as simple conditions, easy operation and low propagation cost, but also can remarkably increase the average main root length of the cut plumbago auriculata, and can greatly improve the survival rate.

The invention discloses a method for increasing bumblebee commodity group propagation speed. The method comprises the following steps: preparing bee species and queen bee, preparing propagation environment and feed, inducing the queen bee to lay eggs, performing transfer box feeding to obtain a bumblebee commodity group, packaging the bumblebee commodity group, and adding worker bees into a feeding box in which the queen bee lives after inducing the queen bee to lay eggs and before performing transfer box feeding. The method for increasing bumblebee commodity group propagation speed provided by the invention shortens the time for propagating single queen bee into the commodity group to 55-65 days, which is shortened by 15 days in comparison to the ordinary propagation time of 70-80 days; the propagation cost is reduced by 20%.

The invention discloses a tissue culture rapid propagation method for excellent sequoia sempervirens seedlings. The method comprises the following steps: constructing explants, disinfecting the explants, performing differential induction, performing enrichment culture, performing ex vitro rooting, transplanting and cultivating. An excellent clone branch of robust annual sequoia sempervirens is selected and is cut into 2-3cm long stems with buds as the explants for tissue culture rapid propagation. The tissue culture rapid propagation method for excellent sequoia sempervirens seedlings disclosed by the invention is slightly influenced by the external environment, is capable of greatly reducing the propagation cost, shortening the propagation period, increasing the propagation coefficient ofthe seedlings and acquiring the seedlings in highly consistent quality, is convenient for large-scale factory cultivation and has important significance in the aspects of seedling propagation and value utilization of the excellent sequoia sempervirens clone in the future.

The invention discloses a cutting rapid propagation method for angelica keiskei and belongs to the field of agricultural technologies. The cutting rapid propagation method for the angelica keiskei is used for simplifying the propagation technology of the angelica keiskei, shortening the propagation period, reducing rapid propagation cost and achieving rapid propagation of the angelica keiskei. The method is mainly used for the medicinal and edible angelica keiskei and includes the detailed steps that stems with the length of 3 cm-5 cm and the thickness of 0.2 cm-0.8 cm are selected and provided with 2-5 compound leaves and 2-5 buds, then the stems are rapidly dipped into rooting powder with 200-400 diluents for 15 seconds-30 seconds, cut into wet sand and watered with sufficient water, under the conditions that the temperature is (25+ / -2) DEG C, the air relative humidity is over 75%, the illumination is 10,000 lx-20,000 lx, and the illumination time is 12 h / d-14 h / d, the stems are cut for 7-15 days, the survival rate is 100%, seedlings which are survival through cutting are transplanted into a land sun-shade shed, the spacing in the rows and the spacing between rows are 20 cm*20 cm, the temperature is 25 DEG C-30 DEG C at daytime, the temperature is 15 DEG C-18 DEG C at night, the air relative humidity is 70%-90%, the shed is appropriately opened for ventilation, and the survival rate reaches 100% at the seventh-tenth day after transplanting. Compared with a tissue culturing rapid propagation technology, the method simplifies the propagation steps, shortens the propagation time and reduces propagation cost, and application and popularization are facilitated.

The invention relates to the technical field of lonicera caerulea propagation, in particular to a lonicera caerulea twig cutting medium seedbed and a cutting propagation method. The lonicera caeruleacutting medium seedbed comprises a substrate layer and a surface layer, wherein the surface layer is positioned on the surface of the substrate layer; the substrate layer comprises fine river sand, larch needles, sawdust and garden soil, and the volume ratio of the fine river sand to the larch needles to the sawdust to the garden soil is (1.8-2.2): (0.4-0.6): (0.4-0.6): (0.8-1.2); and the surfacelayer comprises larch needles and saw dust, and the volume ratio of the larch needles to the saw dust is (0.4-0.6): (0.4-0.6). When the cutting medium seedbed is used for lonicera caerulea cutting propagation, the cutting medium seedbed is good in moisture retention performance, high in nutrient, high in rooting rate and survival rate, capable of being directly used for bed reserving for overwintering (high in preservation rate), low in cost, simple in raw material, suitable for factory-like seedling propagation and particularly beneficial to seedling strengthening and increasing of the survival rate in the bed moving and seedling hardening process.

The invention relates to the technical field cedar planting, in particular to a cedar pollen tissue culture propagation method. The method includes steps: impregnating fruit balls into silver iodide aqueous solution to realize ultrasonic sterilization; performing inoculation of the sterilized fruit balls in a culture medium to realize root and germination induction, wherein the culture medium is formed by an MS culture medium, 1-1.2mg / L epsilon-polylysine, 0.2-0.3mg / L sodium pyrophosphate and 1.8-2mg / L divalent calcium ions; during induction, treating the fruit balls with cold plasmas generated by atmospheric pressure dielectric barrier discharge every 5-6 hours. By adoption of fresh fruit balls as explants for tissue culture propagation, low propagation cost, simple propagation process, high speed in whole tissue culture propagation and short culture period are realized, and large-scale development of seedlings can be realized quickly.