Seedling hardening and domestication method in greenhouse for forest tree tissue culture seedlings

Abstract

The invention relates to a seedling hardening and domestication method in greenhouse for forest tree tissue culture seedlings. The method is characterized by comprising following steps: (1) seedling hardening greenhouse preparation; (2) seedling hardening management of tissue culture seedlings before bottle out; (3) cultivation substrate and container preparation; (4) tissue culture seedling transplanting; (5) domestication management after transplanting; (6) domestication time control after transplanting. According to the seedling hardening and domestication method in greenhouse for forest tree tissue culture seedlings, a uniform and stable domestication environment is formed with white transparent nursery pot used for butterfly orchid production and plates of the corresponding size. Compared with the prior art, the method is simple and efficient; the plates and the nursery pots can be reused, which greatly reduce production cost and can be promoted in large scale; according to the measure integrated effect before, during and after the seedling hardening; the time for seedling hardening after tissue culture seedling transplanting in greenhouse can be shortened by at least one month; the survival rate of seedling hardening is increased by 5 to 15 percentage; the manpower and material cost is reduced by about 30%; the over ground part of the tissue culture seedlings is increased by 30 to 50% compared with seedlings planted by regular methods. The plant grows well.

Description

technical field [0001] The invention belongs to the technical field of rapid propagation of forest trees, and relates to a simple and efficient method for cultivating and acclimating forest tree tissue cultured seedlings in a greenhouse. Background technique [0002] Forest tree group cultivating seedlings is to artificially create the nutritional and environmental conditions suitable for the growth and development of forest trees under sterile conditions, and cultivate the tissues or organs of woody plants to make them an independent plant. The method of cultivating seedlings of the forest tree group is especially suitable for the propagation of rare tree species or horticultural varieties with excellent female parent traits, and because it can obtain virus-free seedlings, it can be used for the preservation of germplasm resources and the production of high-quality virus-free seedlings, which is conducive to promoting The breeding of improved varieties of forest plants is o...

AI Technical Summary

Problems solved by technology

At present, the problems existing in hardening and domestication are: (1) in order to ensure the survival rate, the hardening time in the bottle is too long, which affects the efficiency of seedling growth; (2) the control of environmental conditions is cumbersome and the cost is too high

Tissue culture seedlings for transplanting and hardening need to be carried out in facilities such as smart greenhouses or sheds. The control of temperature, water, and light must be fine, otherwise the roots will rot easily and the survival rate will decrease.

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