33results about How to "Improve the survival rate of hardened seedlings" patented technology

The invention discloses a method for establishing a general maize regeneration system. The method comprises the following steps of: inoculating different maize inbred line embryos onto induction mediums with different hormone combinations, transferring an appropriate induced embryoid onto a differential medium to carry out high frequency differentiation on regenerated seedlings, transferring the regenerated seedlings onto a rooting medium to carrying out rooting induction so as to obtain a regenerated plant, wherein the formula of the induction medium comprises N6 marcoelement, B5 microelement, B5 vitamin, riboflavin (0.04mg / L), folic acid (0.04mg / L), nicotinic acid (0.1mg / L), D-calcium pantothenate (0.1mg / L), methyl benzoic acid (0.04mg / L), cane sugar (50g / L), 2.4-D (0.4mg / L) and 6-BA (5mg / L). The method disclosed by the invention has the characteristics of good generality to different maize inbred lines and shorter time for obtaining the regenerated plant and has important application value.

A tissue culture method for new pteris fern includes such steps as obtain aseptic material, primary culture with the culture medium containing MS, 6-BA, NAA and AC, reproduction culture with the culture medium containing MS, 6-BA and AC, rooting culture with the culture medium containing 1 / 2 MS, NAA and IB, and intelligent culture of seedlings.

The invention discloses an industrial tissue culture and rapid propagation technology for tamarix chinensis. Unified tissue culture and rapid propagation breeding of various tamarix chinensis is realized finally through explant selection, establishment of a disinfection system, multiplication culture, combination of strong seedling culture and rooting culture as well as exercising seedling optimization, several key nodes including initiation rate, quantity of cluster buds on stems, rooting rate, root number, root length and survival rate of exercising seedlings for tissue culture breeding are optimized and controlled, the survival rate of the exercising seedlings with higher consistency is obtained finally, the effects of high initiation rate, many cluster buds, high survival rate and high reproduction coefficient are realized, great convenience and technical support are provided for industrial breeding of the tamarix chinensis, and the technology is quite suitable for industrial breeding production.

The invention discloses a subculture method for tissue culture seedlings of cercis giganter lines. The subculture method is characterized by comprising the following steps: 1, obtaining sterile explants; 2, carrying out subculture multiplication on the cercis giganter lines; 3, carrying out rooting culture on the cercis giganter lines; 4, controlling subculture and rooting culture conditions of the cercis giganter lines; and 5, hardening and transplanting the tissue culture seedlings of the cercis giganter lines. The multiplication method for the tissue culture seedlings of the cercis giganter lines disclosed by the invention has the following advantages that (1) the multiplication coefficient is high and the average multiplication coefficient reaches above 5; (2) the rooting rate is high and the maximum rooting rate can reach 98%; and (3) the survival rate of hardened seedlings is high and averagely above 90%.

The invention discloses a tissue culture method for ilex attenuata 'Sunny Foster'. The method includes the following steps of pretreatment: taking stems of terminal buds or axillary buds of the ilex attenuata 'Sunny Foster', and conducting disinfection and sterilization treatment to obtain a disinfected and sterilized explant; primary culture: inoculating the disinfected and sterilized explant into a primary culture medium for primary culture to obtain axillary buds; proliferation culture: inoculating the axillary buds into a proliferation culture medium for proliferation culture to obtain proliferative seedlings; rooting culture: inoculating single plants of the proliferative seedlings into a rooting culture medium for rooting culture to obtain rooting seedlings. The tissue culture methodhas high seedling hardening survival rate, high reproduction coefficient, simple and convenient tissue culture process and short reproduction time, and is not limited by seasons, and the market demand for the ilex attenuata 'Sunny Foster' is met.

The invention discloses a rapid breeding method of a saline and alkaline resistant poplar. The method comprises the steps including preparation of materials for breeding, collection and storage of a cutting slip, processing of a slip, cutting and management, seedling hardening and transplanting. The cutting slip and the slip are processed by specific cultivation matrix and treatment fluid before cutting. The method is simple in operation and low in cost, the cutting survival rate can reach 98% or higher, the survival rate after cutting and transplantation is high, stress resistance is high, the method can be applied to production directly, and the economical and social values of the method are high.

The invention discloses a method for cultivating chili disease-resistant homozygotes. The method includes a tissue culture identification step and an anti-viral disease screening step. The tissue culture identification is based on conventional culture medium, using trehalose instead of sucrose and adding black wolfberry juice The nutrient composition of the filtrate was formulated to prepare a specific medium formula for the selection of explants, explant treatment, anther culture, acquisition of complete plants, colchicine treatment and ploidy identification of peppers to obtain homozygous seedlings; and use molecular The marker technology is used to screen anti-viral disease molecular markers to obtain anti-viral disease homozygous plants. The method of the present invention can significantly shorten the screening period, and quickly obtain homozygous anti-viral diseases of pepper; and the method is simple in operation, low in cost, high in survival rate after transplanting flower seedlings, strong in stress resistance, easy to popularize, and can be directly applied to production , have good economic and social benefits.

The invention relates to a method for domesticating hardening seedlings of tissue culture seedlings of Nandina domestica firepower. The method is characterized by comprising the following steps of establishing a hardening seedling greenhouse; screening and treating a hardening seedling matrix; managing the tissue culture seedlings before the tissue culture seedlings are bottled out; treating the tissue culture seedlings after the tissue culture seedlings are bottled out; transplanting the hardening seedlings of the tissue culture seedlings; managing the tissue culture seedlings after the hardening seedlings of the tissue culture seedlings are planted; and domesticating the hardening seedlings of the tissue culture seedlings for four months to obtain qualified domestication seedlings of the Nandina domestica firepower. By means of the method for domesticating the hardening seedlings of the tissue culture seedlings of the Nandina domestica firepower, surviving rates of the domestication seedlings are increased form 58% of traditional surviving rates to 83.5% so that the surviving rates of the domestication seedlings of the tissue culture seedlings of the Nandina domestica firepower are greatly increased, the tissue culture seedlings which are obtained by four month domestication of the domestication seedlings can basically adapt to growth environment of the greenhouse, root systems of plants are thick, new twigs are good, stalks are thick, and the surviving rates of the transplanted domestication seedlings can reach 95.8%.

The invention discloses a cutting seedling nutrient solution of lonicera edulis twigs as well as a preparation method and an application method thereof. The cutting seedling nutrient solution includes the following components: 180-220 ml / L honey, 0.9-1.1 ml / L liquid organosilicone, 90-110 ml / L edible vinegar, 45-55 ml / L red orchid extracting solution, 360-440 mg / L pimacol, 36-44 mg / L indolebutyric acid, 9-11 mg / L dichlorphenoxyacetic acid (2, 4-D), 0.9-1.1 g / L potassium dihydrogen phosphate, 0.09-0.11 mg / L iron, 0.045-0.055 mg / L born, 0.036-0.044 mg / L manganese, 0.036-0.044 mg / L copper and 0.027-0.033 mg / L zinc, the cutting seedling nutrient solution disclosed by the invention can be used for preventing decay of a lower notch during a seedling breeding process of the lonicera edulis twigs and promoting quick rooting of an in vitro material.