A kind of fast seedling hardening method of alpine Rhododendron tissue culture seedlings
A technique for tissue culture of seedlings and rhododendrons, applied in the field of plant reproduction, can solve the problems of restricting the rapid promotion of fine varieties, unstable seedling survival rate, uneven growth of seedlings, etc. strong growth effect
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[0024] (1) Alpine rhododendron tissue culture rooting seedling domestication in bottle
[0025] A sunshade net with a shading rate of 50% is installed on the top of the greenhouse, an arch frame with a height of 70 to 80 cm is built on the ground in the greenhouse, and a sunshade net with a shading rate of 50% is covered on the arch. % Long-rooted tissue culture bottle seedlings are placed on the bottom surface under the arch for 15 days without opening the bottle cap; the height of the greenhouse can be 200-400 cm.
[0026] (2) Transplanting of tissue cultured seedlings
[0027] ① Substrate preparation, the substrate is mixed according to the mass ratio of peat: perlite is 1:1, and then 800 times liquid thiophanate-methyl solution is added to the substrate and stirred so that the water content of the substrate is 70% by mass fraction, and then spread into the 540mm×280mm, in a 128-hole tray;
[0028] 2. Take out the tissue-cultured seedlings domesticated in step (1) from th...
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