39 results about "Rhododendron lapponicum" patented technology

The invention relates to a method for cuttage propagation of rhododendron lapponicum, which includes: making a mixture of peat and pearlite into cuttage substrate according to a volute ratio of from 1:3 to 1:4, cutting a stem of a current year growing semi-lignified branch of a rhododendron lapponicum plant which is under no pest and disease damage and 3-5 years old as a cutting, and retaining two to three complete leaves at the top of the cutting; dipping the base of the cutting into rooting reagent A or B prior to inserting into the substrate; and performing intermittent spray for subsequent water management, maintaining mass water content of soil to be 60-65%, and maintaining air humidity to be more than 80% during a period from cuttage to rooting of a cutting seedling. The rooting reagent A includes IBA (indole-3-butyric acid) 0.8-1.0% and thiophanate-methyl 2-4%. The rooting reagent B includes IBA 0.015-0.02% and thiophanate-methyl 1-2%. By the method, rooting rate can reach morethan 85%, rooting of the cutting only requires about 60 days, and production cycle is short.

The invention relates to alpine rhododendron tissue culture quick-propagation method. All the medium is prepared according to following material: medium for inducing explant: 1 / 4MS, MS ferric salt, VitB50.2mg, lactalbumin hydrolysate 300mg, sucrose 30g+Kt1.0mg / L; medium for propagating cluster sprout: 1 / 2MS, Kt0.5mg / L; medium for rooting: 1 / 2MS, NAA5mg / L, active carbon 0.5%. The method of alpine rhododendron tissue culture quick-propagation is characterized as test tube sprout provisional planting method adopting tender stem segment axillary bud culture method and 'two-step' method. The advantages of the invention lie in: the growth is not influenced by season, plant has strong resistance to stress, and the plant has big colorful flowers.

The invention discloses a method for grafting and propagating rhododendron hybrids. In the method, compact rhododendron hybrids are used as scion, and rhododendron cosmopolitan is used as tree stock. The scion is a current-year semi-lignified strong branch which does not have diseases or insect pests, have full eyes, leaves which turn green and ripe, and terminal bud, and does not sprout or just sprouts; and the tree stock is a current-year lignified strong branch which grows luxuriantly, does not have diseases or insect pests, and has smooth and complete skin. In the method, the rhododendron hybrids are grafted by a cleft grafting method; the scion and the tree stock are covered by a plastic bag to maintain moisture, and water drops are prevented from immersing the grafting joint; and sun is shaded properly after grafting, and the illumination intensity is better to be between 90 and 126mu mol.m<-2>.s<-1>, the temperature is better to be between 25 and 28 DEG C, and the air humidity is better to be between 80 and 90 percent. The method is easy to operate, ensures low cost and high survival rate, is favorable for improving the finished product quality of potted rhododendron hybrids, and can be widely applied to commercial production of the rhododendron hybrid.

The invention relates to a flower forcing method enabling blossom of rhododendron lapponicum in Spring Festival. The method comprises the following steps: preparing a greenhouse, processing the potted rhododendron lapponicum before flower forcing, controlling the temperature, humidity, illumination, ventilation, moisture, fertilizers and sterilization management in the greenhouse when buds appear, managing the temperature and humidity when the buds show colors and the like. The method provided by the invention is simple and practical, is easy to operate, can be used for accurately controlling the flower forcing time of the rhododendron lapponicum in Spring Festival within 30-42 days and shortening the flower forcing time and is a key technology in the large-scale production of rhododendron lapponicum.

The invention discloses a rapid seedling hardening method for tissue culture seedlings of rhododendron lapponicum. The method comprises acclimation of tissue culture rooting seedlings in a bottle, transplantation, environment control after transplantation, fertilization management and pest control, wherein the environment control is implemented as follows: a small arch shed is put up below a sunshade net arch structure set up in a large shed, the top surface of the small arch shed is covered with a sunshade net, and a hole tray is put in the small arch shed; the humidity of the small arch shed is kept at 80%-85%, the temperature in the daytime is kept 25 DEG C-30 DEG C, the temperature at night is kept not lower than 5 DEG C, and moisture is controlled; fertilization management and pest control are implemented as follows: after the tissue culture seedlings are transplanted to the hole tray for 30 days, a leaf fertilizer is applied once every two weeks, chlorothalonil or carbendazim is sprayed once every 15 days and sprayed for 3-4 times in total, and imidacloprid is sprayed once every week at the initial stage of aphids in spring and sprayed for 3-4 times in total. By means of the method, the transition time of the tissue culture seedlings of the rhododendron lapponicum is shortened, the survival rate of hardened seedlings is up to 90% or higher, the seedlings grow healthy and strong, a standard seedling hardening technical scheme is provided, and the method is suitable for industrial seedling hardening.

The invention relates to a rhododendron hybrides cosmopolitan tissue culture rapid propagation method which comprises the following steps: A. explant treatment; B. culture initiation; C. multiple shoot culture; D. strong seedling culture, that is, transferring and inoculating aseptic seedlings obtained by the multiple shoot culture to a strong seedling culture medium, and culturing for 75 days at a day temperature of 25+ / -1 DEG C, a night temperature of 22+ / -1 DEG C, and an illumination intensity of 50 [mu]mol.m<-2>.s<-1> and with illumination for 12 h / day; E. rooting culture, that is, culturing for 90 days at a day temperature of 25+ / -1 DEG C, a night temperature of 22+ / -1 DEG C, and an illumination intensity of 50 [mu]mol.m<-2>.s<-1> and with illumination for 12 h / day. The method of the invention is not influenced by time and changes of season or environment, greatly increases the propagation rate, and improves the ornamental value and commercial value of rhododendron hybrides cosmopolitan.

The invention provides a Rhododendron lapponicum garden planting and management method. The Rhododendron lapponicum garden planting and management method includes cutting shoot seedling raising, and planting and management. The cutting shoot seedling-raising includes: taking vegetative shoots of Rhododendron lapponicum as cutting shoots to perform cutting propagation in early autumn, and performing transplanting after the cutting shoots grow into complete seedling plants. The planting and management includes: planting the seedlings of the Rhododendron lapponicum in a semi-shade environment at the temperature of 10-25 DEG C and the humidity of 70-90%; and controlling the planting environment in a growth environment of the Rhododendron lapponicum according to change of seasons and an environment condition during planting, and regularly performing pruning, watering, fertilizing and disease and pest control management. The Rhododendron lapponicum garden planting and management method is feasible, and can achieve large-scale garden planting of the Rhododendron lapponicum.

The invention discloses an improved method for extracting total DNA (deoxyribonucleic acid) from polysaccharide and polyphenol plant Rhododendron lapponicum. A polysaccharide removal treatment step is added to the method, dried Rhododendron lapponicum leaves are ground into powder with liquid nitrogen, then 1 mL of Buffer I is added, and most secondary metabolites such as polysaccharides, polyphenols and the like in a plant cell homogenate are washed through ice bath and centrifugation; a cell lysis solution adopts 4*CTAB (cetyltrimethylammonium bromide); cell lysis mixed liquor is not extracted directly during DNA extraction, but a supernatant obtained from separation after high-speed centrifugation of the cell lysis mixed liquor is extracted; a Rnase A reagent is added to a supernatant obtained through first extraction, the obtained mixture is treated at the room temperature for 20 min, and then second conventional extraction is performed; during washing of DNA precipitates, the arranging direction of a centrifuge tube is changed to the opposite for next washing, and the high-speed centrifugation time is prolonged to 5 min. The method has the advantages of high yield, high purity and good repeatability and can be applied to downstream experiments of molecular biology.

The invention relates to a rhododendron lapponicum flower forcing method in cold regions. The method comprises the following steps of: carrying out low-temperature dormancy treatment on potted rhododendron lapponicum outdoors by utilizing climatic resources in cold regions; carrying out indoor greenhouse space arrangement; breaking dormant flower forcing management, removing lateral buds and spraying 1000ppm of exogenous hormone GA3 to germinating buds; carrying out heating, filling light to regulate room temperature, humidity and illumination in the greenhouse and carrying out water and fertilizer management; and managing the temperature, humidity and water and fertilizer after the buds have colors. The method has the following beneficial effects: by utilizing the peculiar climatic resource advantages in the cold regions to carry out low-temperature dormancy treatment on rhododendron lapponicum outdoors and using variable temperature light filling indoors in combination with the exogenous hormone, the aims of shortening the rhododendron lapponicum flower forcing time in the cold regions and reducing the production cost are achieved; the key problem of rhododendron lapponicum flower forcing in the cold regions is solved; and the technology caters to the lunar new year flower market demands and provides technical support for industrial production of potted flowers in the Heilongjiang province.

The invention belongs to the field of flower planting and particularly discloses a rhododendron lapponicum planting method. The method comprises steps including land selection, control of soil pH, hole digging, plantation, fertilization, management and the like. The problem that rhododendron lapponicum cannot be manually planted in batches because of freezing damage and pest and disease damage is solved through stereoscopic plantation of rhododendron lapponicum, cinnamomum camphora and tamarind pulp and application of water-absorbent resin, nitrogen dioxide and sulfur dioxide, and the economic benefits are improved to a large extent.

The invention belongs to the technical field of ornamental plant biology, and relates to a generating method for a rhododendron delavayi 'cosmopolitan' somatic embryo. The method for inducing generation of the somatic embryo by means of leaves of rhododendron delavayi 'cosmopolitan' mainly comprises the steps that tender leaves are disinfected and then inoculated onto a culture medium containing wpm, 0.2 mg / L of TDZ, 2 mg / L of 2ip, 0.25 mg / L of NAA, sucrose and agar, and after dark culture is conducted, light culture is conducted. According to the inducing method for the ornamental plant rhododendron delavayi 'cosmopolitan' somatic embryo, a foundation is laid for research on reestablishment of a rhododendron delavayi genetic transformation system, protoplast culture, excellent clone propagation genetic engineering and / or mutant screening and the like.

The invention discloses a method for cultivating aromatic alpine rhododendron. The method comprises the following steps: selecting a rhododendron plant amethyst (female) which is not suffering from pest and disease damage, is strong in growth vigor and is in an early flowering period and rhododendron decorum (male) as cross parents, selecting just colored flowers of the rhododendron decorum in the early of middle of April, removing petals and stamens at about 10a.m, only remaining pistils, pollinating the pistils with pollen of the amethyst and bagging, removing the capped bags within 7-10 days, picking fruits when the fruits turn yellow in the middle of November, and sowing the fruit in a greenhouse in the middle of December; by taking pure peat soil as a sowing medium, thoroughly watering the soil before seeding, cover the soil with a film and moisturizing until seedlings come out; transplanting the seedlings to a 50-mesh tray when 5-6 leaves of the seedlings grow, recovering the seedlings, pinching, and fertilizing the seedlings with a 0.2 percent Huaduoduo balance fertilizer once every 10-15 days; and planting the seedlings in nutrition bags after 3-4 months, and performing regular management.

The invention provides an indoor annual sowing and seedling raising method for rhododendron lapponicum, and belongs to the technical field of plant sowing and seedling raising. The seedling raising method comprises the following steps: collecting seeds; treating a sowing substrate; disinfecting the sowing substrate with a bactericide for later use; sowing: placing the sown seedling raising tray under an indoor growth light source for seedling raising; germination period management: supplementing water to keep the substrate moist in the seed germination process; seedling transplanting and seedling stage management: transplanting seedlings in a seedling raising tray into a hole tray with a seedling raising substrate, planting 1-3 seedlings in each hole, thoroughly watering the seedlings after the seedlings are transplanted, placing the seedlings under an indoor growth light source, keeping sufficient water during seedling raising, and preventing and treating diseases and insect pests; transplanting the seedlings, namely transplanting the seedlings in the hole tray into a planting container with a transplanting substrate, so as to obtain the alpine rhododendron sown seedlings. According to the method, sowing time is not limited, management is convenient, and the seedling rate is increased.

The invention discloses a bud stripping and sterilizing method of Rhododendron lapponicum explants. The method mainly includes: removing the top leaf bud and flower bud of a branch for bud taking to promote the germination of lateral buds at the top leaf bud and the petiole of the branch; after the branch grows on a stock plant for 10-30 days and the lateral buds at the top leaf bud and the petiole germinate and when leaves do not completely spread, sterilizing the leaf bud and the lateral buds, and performing bud stripping once before and after the sterilizing to obtain the bud-stripped and sterilized explants, to be more specific, stripping 1-2 layers leaves, from outside to inside, of a bract and the buds. The bud stripping and sterilizing method has the advantages that the tissue culture pollution rate of the bud-stripped and sterilized explants is not higher than 30%, the bud induction survival rate of the bud-stripped and sterilized explants is higher than 63%, the technical bottleneck that the explants of Rhododendron lapponicum whose surface is densely covered by villi and / or glands are difficult to sterilize during the building of the plant regeneration system of the Rhododendron lapponicum is broken through, the building speed of the plant regeneration system of the Rhododendron lapponicum is increased, and technical support is provided for Rhododendron fundamental researches, seedling industrial production and protection of rare and endangered species.

The invention discloses an abnormal temperature germination accelerating process for rhododendron molle seeds, and particularly relates to the technical field of germination accelerating processes. When the rhododendron molle seeds are subjected to seedling acceleration, the rhododendron molle seeds are placed in a constant-temperature room for abnormal temperature seedling acceleration, meanwhile, the proper humidity and oxygen concentration around the rhododendron molle seeds are maintained, and the method greatly shortens the germination period of the rhododendron lapponicum seeds in a natural state, improves the germination rate of the rhododendron lapponicum seeds, can improve the seedling survival rate of the rhododendron lapponicum seeds through warm water disinfection and sterilization treatment, reduces the infection of diseases to the seeds and buds, meanwhile, reduces the production cost, is convenient to popularize and use, and provides a basis for popularizing planting of the rhododendron molle; and gamma rays are adopted to irradiate the seeds, the germination rate of the seeds is promoted, meanwhile, temperature-changing treatment is adopted to treat the rhododendron molle seeds, the protoplast viscosity of radicles of the rhododendron molle seeds is enhanced, the adaptability to low temperature is enhanced, and the stress resistance is enhanced.

The invention relates to a rhododendron lapponicum flower forcing method in cold regions. The method comprises the following steps of: carrying out low-temperature dormancy treatment on potted rhododendron lapponicum outdoors by utilizing climatic resources in cold regions; carrying out indoor greenhouse space arrangement; breaking dormant flower forcing management, removing lateral buds and spraying 1000ppm of exogenous hormone GA3 to germinating buds; carrying out heating, filling light to regulate room temperature, humidity and illumination in the greenhouse and carrying out water and fertilizer management; and managing the temperature, humidity and water and fertilizer after the buds have colors. The method has the following beneficial effects: by utilizing the peculiar climatic resource advantages in the cold regions to carry out low-temperature dormancy treatment on rhododendron lapponicum outdoors and using variable temperature light filling indoors in combination with the exogenous hormone, the aims of shortening the rhododendron lapponicum flower forcing time in the cold regions and reducing the production cost are achieved; the key problem of rhododendron lapponicum flower forcing in the cold regions is solved; and the technology caters to the lunar new year flower market demands and provides technical support for industrial production of potted flowers in the Heilongjiang province.

The invention relates to a method for cultivating tree-shaped alpine rhododendron. The method comprises the steps of stock cultivation, establishment and management of an alpine rhododendron scion collection orchard, grafting, management after grafting and the like. According to the method for cultivating tree-shaped alpine rhododendron disclosed by the invention, rhododendron delavayi is adoptedas a stock, compact alpine rhododendron is adopted as a scion, and the scion and the cambium of the stock are tightly combined during grafting, so that the survival rate is high, the vitality is high,the plant type is good, and shaping can be achieved just by proper pruning in later-period management. The method is simple, practical, low in cost and high in product quality consistency, can be used for large-scale production and cultivation of high-quality tree-shaped alpine rhododendron, can realize batch localization of the tree-shaped alpine rhododendron, solves the problem of commercialization of the tree-shaped alpine rhododendron in China and changes the situation that the tree-shaped alpine rhododendron depends on imports.

The invention relates to the technical field of lateral root induction, in particular to a technical method for lateral root induction of rhododendron lapponicum, The technical method comprises the following steps: S1, branch selection: removing tail ends with rolled leaves from the rhododendron lapponicum, and picking off flower buds to obtain branches for later use; S2, sterilization treatment: soaking the roots of the branches in a thiophanate methyl solution for more than half a day, and taking out the branches for natural air drying after disinfection; and S3, preparation of a nutrient solution: uniformly mixing ABT rooting powder and gibberellin with water in proportion to prepare a plant growth regulator, adding bacillus and an ultraviolet absorption aid into the plant growth regulator, and uniformly stirring and mixing to obtain the nutrient solution. According to the method, the inhibition effect of the rhododendron lapponicum on ultraviolet rays can be improved, the activity of bacillus is guaranteed, the root development of the rhododendron lapponicum can be remarkably promoted, and in addition, the wettability of the surface of the rhododendron lapponicum can be improved, so that the effect of the medicine is improved.

The invention relates to a flower forcing method enabling blossom of rhododendron lapponicum in Spring Festival. The method comprises the following steps: preparing a greenhouse, processing the potted rhododendron lapponicum before flower forcing, controlling the temperature, humidity, illumination, ventilation, moisture, fertilizers and sterilization management in the greenhouse when buds appear, managing the temperature and humidity when the buds show colors and the like. The method provided by the invention is simple and practical, is easy to operate, can be used for accurately controllingthe flower forcing time of the rhododendron lapponicum in Spring Festival within 30-42 days and shortening the flower forcing time and is a key technology in the large-scale production of rhododendron lapponicum.

The invention discloses a bud stripping and sterilizing method of Rhododendron lapponicum explants. The method mainly includes: removing the top leaf bud and flower bud of a branch for bud taking to promote the germination of lateral buds at the top leaf bud and the petiole of the branch; after the branch grows on a stock plant for 10-30 days and the lateral buds at the top leaf bud and the petiole germinate and when leaves do not completely spread, sterilizing the leaf bud and the lateral buds, and performing bud stripping once before and after the sterilizing to obtain the bud-stripped and sterilized explants, to be more specific, stripping 1-2 layers leaves, from outside to inside, of a bract and the buds. The bud stripping and sterilizing method has the advantages that the tissue culture pollution rate of the bud-stripped and sterilized explants is not higher than 30%, the bud induction survival rate of the bud-stripped and sterilized explants is higher than 63%, the technical bottleneck that the explants of Rhododendron lapponicum whose surface is densely covered by villi and / or glands are difficult to sterilize during the building of the plant regeneration system of the Rhododendron lapponicum is broken through, the building speed of the plant regeneration system of the Rhododendron lapponicum is increased, and technical support is provided for Rhododendron fundamental researches, seedling industrial production and protection of rare and endangered species.

The invention discloses an efficient rooting method for rhododendron lapponicum tissue culture seedlings. A pure water moss matrix planting technology is adopted to cultivate the rhododendron lapponicum tissue culture seedlings, so that the rooting efficiency of the tissue culture seedlings is improved to 90%, the number of lateral roots is obviously improved, the survival rate is improved to 95%, the rooting time is shortened, the operation is convenient, and the efficient rooting method is beneficial to industrial large-area popularization.

A low-carbon treatment method for alpine rhododendron potted flowers in autumn in central Yunnan, including selecting alpine rhododendron potted flowers with current-year branches and full buds on the branches in early and mid-June, and treating alpine rhododendrons every 7 to 10 days. Spray fungicides on the whole plant. In late June, place the alpine rhododendron potted flowers in a cold storage at 3-4°C for refrigeration, and put the alpine rhododendron potted flowers in a greenhouse for maintenance. Water, spray 300-400 times liquid amino acid-containing water-soluble fertilizer every 10-12 days, maintain for 4-6 weeks, and the flower buds will open to reach the expected flowering goal. This method enables Rhododendron alpine to bloom in September-November of the same year under the condition of low energy consumption without heating treatment and supplementary light treatment, effectively prolonging the flowering period of Rhododendron alpine and providing more benefits for producers. Provide consumers with more choices.

The invention discloses an efficient genetic transformation method of whole strain infection method mediated by Agrobacterium tumefaciens of Rhododendron alpinum. The method mainly comprises the following steps: seed pre-culture, ultrasonic treatment, preparation of Agrobacterium tumefaciens bacterial solution, agrobacterium infection and vacuum filtration, co-culture, degerming washing, germination culture, GUS histochemical staining. The method of the invention has the advantages of simple operation, low experiment cost, short transformation period and high genetic transformation efficiencyof 61%, and can be applied to the genetic transformation of other plants of Rhododendron.

The invention relates to the technical field of rhododendron cultivation, and particularly discloses a flat ground cultivation method of alpine rhododendron, which comprises the following steps: selecting pine needles, sawdust, pine barks, cottonseed hulls, perlite, vermiculite, turf and peat soil, proportioning, sieving, sterilizing, mixing and stirring to form a soil matrix, digging alpine rhododendron plants, protecting root systems of the alpine rhododendron plants, and planting the alpine rhododendron plants into the soil matrix. The method comprises the following steps of: transporting alpine rhododendron plants to the flat ground under a sufficient oxygen environment, and cultivating the alpine rhododendron plants on a foundation pit in a soil matrix, and keeping the soil around the soil matrix cold and wet. According to the flat ground cultivation method for the alpine rhododendron, the soil matrix is formed through mixing and stirring, the alpine rhododendron is cultivated in the soil matrix, alpine soil is simulated to the maximum extent, nutrients can be continuously absorbed from an original soil mass after the alpine rhododendron survives for a period of time, and the situation that the survival rate of the alpine rhododendron is reduced after the nutrients in the original soil mass are exhausted is prevented.

The invention relates to a soilless culture method for promoting rapid flowering of seedlings of an alpine rhododendron pink lady, wherein the seedlings of the alpine rhododendron pink lady are hybridoffspring seedlings of the alpine rhododendron pink lady; the method comprises the following steps of: preparing a culture medium; planting the hybrid offspring seedlings of the alpine rhododendron pink lady; carrying out bud removal treatment, transplanting, cultivation environment control, fertilization measures and watering measures. By adopting the soilless culture method for promoting rapidflowering of seedlings of the alpine rhododendron pink lady, the hybrid offspring seedlings of the alpine rhododendron pink lady can grow healthily, the plants are strong, the time from planting to flowering only needs 2 years and 7 months to 2 years and 8 months, and the flowering time is shortened by 1 to 2 years compared with the traditional planting method; the method can shorten the whole breeding period of the hybrid offspring of the alpine rhododendron pink lady by 2-4 years, and greatly shortens the breeding period of a new alpine rhododendron variety.

The invention discloses a rapid seedling hardening method for rhododendron lapponicum tissue culture seedlings. The rapid seedling hardening method comprises the following steps of (1) conducting bottle seedling hardening, selecting a south slope of a coniferous forest with the altitude of 1000-1900m, the air humidity of more than 50% and the east-west trend, controlling the light transmittance of the coniferous forest to be 40-60%, building a bottle seedling hardening greenhouse under the forest by using a sunshade net and an arch frame, and carrying out temperature-adaptive seedling hardening; (2) performing field planting and seedling hardening on the tissue culture seedlings after bottle opening, selecting a south slope of a coniferous forest with the altitude of 2000-3500m, the air humidity of 60% or above, the soil layer thickness of more than 40cm, the gradient of less than 45 degrees and the east-west trend, controlling the light transmittance of the coniferous forest to be 50-80%, building a bottle opening, field planting and seedling hardening greenhouse under the forest through the sunshade net and the arch frame, preparing a substrate, after taking out the tissue culture seedlings subjected to bottle seedling hardening in the (1), dipping the roots of the tissue culture seedlings with an 800ppm indolebutyric acid solution, then conducting field planting on the tissue culture seedlings in the substrate, conducting watering and root fixing, and conducting final-period management. The method can obviously improve the transplanting survival rate of the tissue culture seedlings.

The invention relates to an alpine rose branching accelerant and belongs to the technical field of plant cultivation. The alpine rose branching accelerant comprises the following components in parts by weight: 1-2.5 parts of urea, 0.5-1.5 parts of potassium nitrate, 0.003-0.01 part of 5-nitroguaiacol sodium and 5-15 parts of glucose. The alpine rose branching accelerant is prepared in the manner of dissolving the formula components into 1000 parts of deionized water. The alpine rose branching accelerant is applied for the first time when the terminal buds of alpine rose expand in spring in each year, the alpine rose branching accelerant is applied for the second time per 5-7 days, and meanwhile, the normal water fertilizer of the alpine rose is guaranteed. The alpine rose branching accelerant provided by the invention can promote the alpine rose branching, so that the cultivated alpine rose can become luxuriant, has higher ornamental value and excellent commodity character and can meet the requirement of the market for high-quality alpine rose, the income of the producer can be increased and the alpine rose branching accelerant is beneficial to the efficient production of the alpine rose.