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A kind of bud stripping disinfection method of alpine rhododendron explant

A disinfection method and explant technology, which is applied in the field of plant tissue culture, can solve the problems of high disinfection pollution rate and death rate, and hinder the plant regeneration system, so as to achieve good disinfection effect, reduce use time, and reduce damage.

Active Publication Date: 2019-10-22
FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the bottleneck problem that the alpine rhododendron explants whose body surface is densely covered with downy hairs or / and glands have high disinfection pollution rate and mortality rate and hinder the establishment of its plant regeneration system, the invention provides a bud-stripping disinfection of rhododendron explants method

Method used

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  • A kind of bud stripping disinfection method of alpine rhododendron explant
  • A kind of bud stripping disinfection method of alpine rhododendron explant
  • A kind of bud stripping disinfection method of alpine rhododendron explant

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Effect test

Embodiment 1

[0022] Embodiment 1 The inventive method is to the bud stripping disinfection of Rhododendron lantana explant

[0023] (1) get the pretreatment of bud branch

[0024] In April, select the branches of Rhododendron lantana that grow vigorously and have full side buds, keep their branches to grow on the mother plant, and only remove the top leaf buds and / or flower buds at the top of the branches (that is, after removing the leaf buds and / or after the flower buds at the top of the Rhododendron lantana branch, the Rhododendron lantana branch still continues to grow on its mother plant) to promote the germination of leaf buds at the top of its branches and / or the germination of side buds at the petiole (see figure 1 , figure 2 ).

[0025] (2) Taking buds and peeling buds for the first time

[0026] The rhododendron lantana branch after pretreatment in step (1) grows on its mother plant for 15 days, and the top leaf bud of the branch after the pretreatment and / or the side bud a...

Embodiment 2

[0031] Embodiment 2 The inventive method is to the bud stripping disinfection of Rhododendron pachyrhiza explant

[0032] Embodiment 2 is the same as Embodiment 1 except that the following measures are different, and will not be repeated here.

[0033] (1) get the pretreatment of bud branch

[0034] The pretreated bud-taking branches are Rhododendron pachyrhiza branches.

[0035] (2) Taking buds and peeling buds for the first time

[0036] Treat step (1) after the pretreatment Rhododendron pachyrhiza branch grows on its mother plant for 25 days, get buds and carry out bud stripping for the first time by the operation of embodiment 1 step (2).

[0037] (3) Sprout disinfection

[0038] The time for immersion disinfection in 0.1% mercury chloride solution is 12 minutes.

[0039] (4) Second peeling and inoculation

[0040] Cut off the 0.2-0.5mm stem section at the base of the bud body to obtain the explant of Rhododendron pachyphyllum explants stripped and sterilized, and the...

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Abstract

The invention discloses a bud stripping and sterilizing method of Rhododendron lapponicum explants. The method mainly includes: removing the top leaf bud and flower bud of a branch for bud taking to promote the germination of lateral buds at the top leaf bud and the petiole of the branch; after the branch grows on a stock plant for 10-30 days and the lateral buds at the top leaf bud and the petiole germinate and when leaves do not completely spread, sterilizing the leaf bud and the lateral buds, and performing bud stripping once before and after the sterilizing to obtain the bud-stripped and sterilized explants, to be more specific, stripping 1-2 layers leaves, from outside to inside, of a bract and the buds. The bud stripping and sterilizing method has the advantages that the tissue culture pollution rate of the bud-stripped and sterilized explants is not higher than 30%, the bud induction survival rate of the bud-stripped and sterilized explants is higher than 63%, the technical bottleneck that the explants of Rhododendron lapponicum whose surface is densely covered by villi and / or glands are difficult to sterilize during the building of the plant regeneration system of the Rhododendron lapponicum is broken through, the building speed of the plant regeneration system of the Rhododendron lapponicum is increased, and technical support is provided for Rhododendron fundamental researches, seedling industrial production and protection of rare and endangered species.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a disinfection method for alpine rhododendron explants densely covered with villi or / and glands on the body surface, and can be widely used in the basic research of rhododendrons and plants in industrialized production of seedlings The explant disinfection link established by the regeneration system. Background technique [0002] The establishment of a high-efficiency plant regeneration system is the technical basis for the basic research of rhododendron genetic engineering breeding, the stable propagation of hybrid offspring, as well as the industrial production of rhododendron seedlings and the conservation of rare and endangered species. The above applications all require that the rhododendron plant regeneration system uses vegetative organs (such as buds and stem segments) as explants to maintain the consistency of the reproductive offspring with the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 彭绿春宋杰李世峰解玮佳李树发蔡艳飞张露
Owner FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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