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Efficient rooting method for rhododendron lapponicum tissue culture seedlings

A technique for tissue culture seedlings and rhododendrons, applied in the field of efficient rooting of alpine rhododendron tissue culture seedlings, can solve the problems of high vitrification rate, difficult rooting, easy browning of explants, etc., and achieve the effect of large number and increased survival rate

Inactive Publication Date: 2021-12-10
VEGETABLE RES INST OF SHANDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the tissue culture of Alpine Rhododendron usually has a series of problems such as easy browning of explants, high vitrification rate, and difficulty in rooting.

Method used

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  • Efficient rooting method for rhododendron lapponicum tissue culture seedlings
  • Efficient rooting method for rhododendron lapponicum tissue culture seedlings
  • Efficient rooting method for rhododendron lapponicum tissue culture seedlings

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Experimental program
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Effect test

Embodiment 1

[0023] Embodiment 1 pure sphagnum moss substrate planting technology and existing containing a small amount of sphagnum moss substrate planting technology

[0024] 1. Experimental steps

[0025] 1) Materials and treatment: Select healthy and pollution-free Rhododendron alpine tissue culture cluster buds (such as figure 1 ), on the ultra-clean workbench, cut about 3-5cm with a scalpel, and the sprouts of 4-5 leaves are inoculated into the buffered rooting medium, the buffered medium is: WPM basic medium, add NAA:IBA= 0.25mg / L: 0.25mg / L, soft white sugar 30g / L, activated carbon 1g / L, agar powder 6g / L. After cultivating in dark for 7 days;

[0026] 2) Place the tissue culture bottle in an intelligent greenhouse at a temperature of 25°C-28°C, cover it with white non-woven fabric, harden the seedlings for 3 days, take out the sprouts, soak them in 800 times carbendazim disinfectant for 15 minutes, and place them on a newspaper Set aside after drying;

[0027] 3) Pure sphagnum m...

Embodiment 2

[0034] 1. Materials: Healthy and pollution-free alpine rhododendron tissue culture cluster buds

[0035] 2. Method

[0036] (1) Treatment method of clustered buds of Rhododendron alpine: Use a scalpel to cut about 3-5cm and 4-5 leaf buds on the ultra-clean bench, inoculate them into the buffered rooting basic medium: WPM basic medium, add soft white sugar 30g / L, gac 1g / L, agar powder 6g / L), add 4 kinds (g1, g2, g3, g4) in the buffer rooting medium of different hormone ratios simultaneously, wherein (g1=(NAA:IBA=1.5 :0.5), g2=(NAA:IBA=1.0:0.5), g3=(NAA:IBA=0.5:0.5), g4=(NAA:IBA=0.25:0.25)), put 8 cluster buds in every bottle, Five bottles of each medium were inoculated, repeated three times, and then the treatment was placed in the tissue culture room, under dark conditions, for 7 days, and the culture temperature was 25-28°C.

[0037] (2) After 7 days, place the tissue culture bottle in an intelligent greenhouse at a temperature of 25°C-30°C, cover it with a white non-woven ...

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Abstract

The invention discloses an efficient rooting method for rhododendron lapponicum tissue culture seedlings. A pure water moss matrix planting technology is adopted to cultivate the rhododendron lapponicum tissue culture seedlings, so that the rooting efficiency of the tissue culture seedlings is improved to 90%, the number of lateral roots is obviously improved, the survival rate is improved to 95%, the rooting time is shortened, the operation is convenient, and the efficient rooting method is beneficial to industrial large-area popularization.

Description

technical field [0001] The invention relates to a method for efficiently rooting alpine rhododendron tissue culture seedlings, belonging to the technical field of plant tissue culture. Background technique [0002] Alpine Rhododendron (Rhododendron Delavayi) refers to the evergreen rhododendron in the subgenus Rhododendron delavayi, the subgenus Rhododendron delavayi, and the evergreen rhododendron in the subgenus Rhododendron delavayi. . Originally produced in the dense forests of the central and western regions of my country, it was introduced, cultivated and domesticated by Germany, Belgium and other countries, and many excellent varieties were bred through improvement and selection. The ornamental value is very high, and it has become very popular in the domestic and foreign flower markets in recent years. Alpine Rhododendron is usually propagated by cuttings and grafting, but many rare varieties are difficult to root and survive through grafting. At the same time, due ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G31/00A01G24/22A01G24/28A01G24/15
CPCA01H4/008A01H4/002A01G31/00A01G24/22A01G24/28A01G24/15
Inventor 朱娇吕晓惠董飞王烨楠齐宇王成鹏温超马蕾
Owner VEGETABLE RES INST OF SHANDONG ACADEMY OF AGRI SCI
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