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Rapid seedling hardening method for rhododendron lapponicum tissue culture seedlings

A tissue culture seedling and seedling refining technology, applied in the field of plant reproduction, can solve the problems of low survival rate of transplanting alpine Rhododendron tissue culture seedlings, and achieve the effects of neat growth, strong environmental adaptability and robust growth.

Pending Publication Date: 2021-06-22
云南海达新生态环境建设有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a method for fast seedling hardening of alpine rhododendron tissue culture seedlings, which solves the technical problem that the alpine rhododendron tissue culture seedlings have a low transplant survival rate after rooting culture

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Test location: a coniferous forest farm in a certain place in Yunnan

[0025] A kind of fast seedling hardening method of rhododendron alpine tissue culture seedling, comprises the steps:

[0026] (1) bottle seedling hardening

[0027] Select the south-facing slope of the coniferous forest with an altitude between 1000m-1900m, an air humidity of more than 50%, and an east-west trend; cut down the undergrowth of the coniferous forest, and thinn the dense forest trees to make the light transmittance 60%; Under the forest, use sunshade nets and arches to build greenhouses for bottle seedling hardening, and the shading rate of the shade nets for bottle seedling hardening greenhouses is 60%;

[0028] More than 60% of the rooted unopened tissue culture bottle seedlings are placed in the bottle seedling hardening shed built in step (1) and placed naturally to carry out temperature-adaptive hardening, until the tissue culture seedlings take root, and the roots The length is 1...

Embodiment 2

[0036] Test location: a coniferous forest farm in a certain place in Yunnan

[0037] A kind of fast seedling hardening method of rhododendron alpine tissue culture seedling, comprises the steps:

[0038] (1) bottle seedling hardening

[0039] Select the south-facing slope of the coniferous forest with an altitude between 1000m-1900m, an air humidity of more than 50%, and an east-west trend; cut down the undergrowth of the coniferous forest, and thinn the dense forest trees to make the light transmittance 40%; Under the forest, use sunshade nets and arches to build greenhouses for bottle seedling hardening, and the shading rate of the shade nets for bottle seedling hardening greenhouses is 60%;

[0040] More than 60% of the rooted unopened tissue culture bottle seedlings are placed in the bottle seedling hardening shed built in step (1) and placed naturally to carry out temperature-adaptive hardening, until the tissue culture seedlings take root, and the roots The length is 2...

Embodiment 3

[0048] Test site: a coniferous forest farm in a certain place in Yunnan and a coniferous forest farm in a certain place in Guizhou

[0049] A kind of fast seedling hardening method of rhododendron alpine tissue culture seedling, comprises the steps:

[0050](1) bottle seedling hardening

[0051] In a coniferous forest farm in a certain place in Guizhou, select the southward slope of the coniferous forest with an altitude between 1000m-1900m, an air humidity of more than 50%, and an east-west trend; cut down the undergrowth of the coniferous forest, and thinn the dense forest trees, so that The light transmittance is 50%; under the forest, use the sunshade net and the arch frame to build a greenhouse for bottle seedling hardening, and the shading rate of the shade net for bottle seedling hardening greenhouse is 70%;

[0052] More than 60% of the rooted unopened tissue culture bottle seedlings are placed in the bottle seedling hardening shed built in step (1) and placed natural...

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Abstract

The invention discloses a rapid seedling hardening method for rhododendron lapponicum tissue culture seedlings. The rapid seedling hardening method comprises the following steps of (1) conducting bottle seedling hardening, selecting a south slope of a coniferous forest with the altitude of 1000-1900m, the air humidity of more than 50% and the east-west trend, controlling the light transmittance of the coniferous forest to be 40-60%, building a bottle seedling hardening greenhouse under the forest by using a sunshade net and an arch frame, and carrying out temperature-adaptive seedling hardening; (2) performing field planting and seedling hardening on the tissue culture seedlings after bottle opening, selecting a south slope of a coniferous forest with the altitude of 2000-3500m, the air humidity of 60% or above, the soil layer thickness of more than 40cm, the gradient of less than 45 degrees and the east-west trend, controlling the light transmittance of the coniferous forest to be 50-80%, building a bottle opening, field planting and seedling hardening greenhouse under the forest through the sunshade net and the arch frame, preparing a substrate, after taking out the tissue culture seedlings subjected to bottle seedling hardening in the (1), dipping the roots of the tissue culture seedlings with an 800ppm indolebutyric acid solution, then conducting field planting on the tissue culture seedlings in the substrate, conducting watering and root fixing, and conducting final-period management. The method can obviously improve the transplanting survival rate of the tissue culture seedlings.

Description

technical field [0001] The invention relates to the technical field of plant propagation, in particular to a method for rapid seedling hardening of rhododendron alpine tissue culture seedlings. Background technique [0002] Seedling hardening is the process of forcibly tempering seedlings by taking measures such as venting, cooling, and proper water control under the condition of seedling cultivation in protected land, so that they can quickly adapt to the adverse environmental conditions on land after planting, shorten the time for seedling slowing down, and enhance the resistance to low temperature, Resistance to strong winds, etc. Seedling hardening is an essential link for transplanting and domesticating tissue-cultured seedlings to the field. The success of hardening is directly related to the survival rate of tissue-cultured seedlings for transplanting and domesticating. [0003] Alpine Rhododendron is a general term for evergreen Rhododendron and its new horticultura...

Claims

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Application Information

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IPC IPC(8): A01G22/60
CPCA01G22/60
Inventor 刘东风
Owner 云南海达新生态环境建设有限公司
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