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Rhododendron hybrides cosmopolitan tissue culture rapid propagation method

A technology of pink king butterfly and rhododendron, applied in the field of plant cultivation, can solve problems such as low survival rate, difficulty in large-scale production, etc., and achieve the effects of improving reproduction rate, improving ornamental value and commercial value, and protecting excellent germplasm resources.

Inactive Publication Date: 2014-03-19
SHIJIAZHUANG ACADEMY OF AGRI & FORESTRY SCI +1
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Problems solved by technology

Alpine Rhododendron is propagated by seeding, cuttings, layering, and grafting. However, these techniques are limited by many factors such as seasons and mother plant materials. The survival rate is extremely low, and it is difficult to carry out large-scale production.
Tissue culture of alpine Rhododendron only involves a few varieties such as Rhododendron delavayi Franch, Rhododendron Parvifolium Adoms, Rhododendron Chrysantiuum pall, etc. There is no report at home and abroad on the tissue culture of alpine Rhododendron delavayi. Research

Method used

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  • Rhododendron hybrides cosmopolitan tissue culture rapid propagation method
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  • Rhododendron hybrides cosmopolitan tissue culture rapid propagation method

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Embodiment Construction

[0014] The method steps of the present invention are described in detail below in conjunction with specific examples,

[0015] The alpine Rhododendron azalea variety was introduced from Ghent, Belgium in November 2009, and then domesticated in the Shijiazhuang Academy of Agriculture and Forestry Sciences for nearly 4 years. This method of rapid propagation of alpine Rhododendron azalea through tissue culture includes the following steps:

[0016] A. Explant treatment: select robust and harmless alpine Rhododendron chinensis with bud stems as explants, soak them in 75% alcohol for 30 seconds, wash them with sterile water for 3 times, and then sterilize them with 0.1% mercuric chloride. Bacteria for 3 minutes, washed with sterile water for 3 times;

[0017] B. Start-up culture: inoculate the sterilized explants into the start-up culture medium, the daytime temperature is 25±1°C, the nighttime is 22±1°C, the light intensity is 50μmol·m-2·s-1, and the light is 12h per day. The cu...

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Abstract

The invention relates to a rhododendron hybrides cosmopolitan tissue culture rapid propagation method which comprises the following steps: A. explant treatment; B. culture initiation; C. multiple shoot culture; D. strong seedling culture, that is, transferring and inoculating aseptic seedlings obtained by the multiple shoot culture to a strong seedling culture medium, and culturing for 75 days at a day temperature of 25+ / -1 DEG C, a night temperature of 22+ / -1 DEG C, and an illumination intensity of 50 [mu]mol.m<-2>.s<-1> and with illumination for 12 h / day; E. rooting culture, that is, culturing for 90 days at a day temperature of 25+ / -1 DEG C, a night temperature of 22+ / -1 DEG C, and an illumination intensity of 50 [mu]mol.m<-2>.s<-1> and with illumination for 12 h / day. The method of the invention is not influenced by time and changes of season or environment, greatly increases the propagation rate, and improves the ornamental value and commercial value of rhododendron hybrides cosmopolitan.

Description

technical field [0001] The invention relates to a tissue culture rapid propagation method of alpine Rhododendron azalea, belongs to the technical field of plant cultivation, and is mainly used for large-scale breeding of alpine rhododendron new varieties. Background technique [0002] Rhododendron hybrides "Cosmopolitan" is a hybrid cultivar of Rhododendron family Rhododendron. It is an evergreen shrub and small tree with pink flowers and fading to pink and white edges, with a large brown red spot. The plants are dense and the plant type is Stretch, bright green leaves, cold-resistant and heat-resistant, no leaves falling at -23°C, strong disease resistance, with high ornamental value. Alpine Rhododendron is propagated by sowing, cuttings, layering, and grafting. However, these techniques are limited by many factors such as seasons and mother plant materials. The survival rate is extremely low, and it is difficult to carry out large-scale production. Tissue culture of alpin...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 李志斌张铁石蒋淑磊徐立军白霄霞李振勤徐秋良孙春峰姜贵平张文芝
Owner SHIJIAZHUANG ACADEMY OF AGRI & FORESTRY SCI
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