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Rapid seedling hardening method for tissue culture seedlings of rhododendron lapponicum

A technique for tissue culture of seedlings and rhododendrons, applied in the field of plant reproduction, can solve the problems of restricting the rapid promotion of fine varieties, unstable seedling survival rate, poor operability and repeatability, etc. The effect of tissue culture seedling survival

Active Publication Date: 2015-12-30
FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved in the present invention is aimed at the low survival rate of hardened seedlings in the existing alpine rhododendron tissue culture seedling hardening technology, the slow growth of hardened seedlings, uneven growth of seedlings, and the hardening of seedlings largely depends on the seedling growers. Seedling cultivation experience, the survival rate of hardened seedlings is extremely unstable, and the repeatability of the operation is poor, which limits the rapid promotion of good varieties. The method of rapid hardening of alpine Rhododendron alpine tissue culture seedlings with improved survival rate of transplanted seedlings, neat growth, feasible operation, and high repeatability

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Alpine rhododendron tissue culture rooting seedling domestication in bottle

[0025] A sunshade net with a shading rate of 50% is installed on the top of the greenhouse, an arch frame with a height of 70 to 80 cm is built on the ground in the greenhouse, and a sunshade net with a shading rate of 50% is covered on the arch. % Long-rooted tissue culture bottle seedlings are placed on the bottom surface under the arch for 15 days without opening the bottle cap; the height of the greenhouse can be 200-400 cm.

[0026] (2) Transplanting of tissue cultured seedlings

[0027] ① Substrate preparation, the substrate is mixed according to the mass ratio of peat: perlite is 1:1, and then 800 times liquid thiophanate-methyl solution is added to the substrate and stirred so that the water content of the substrate is 70% by mass fraction, and then spread into the 540mm×280mm, in a 128-hole tray;

[0028] 2. Take out the tissue-cultured seedlings domesticated in step (1) from th...

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Abstract

The invention discloses a rapid seedling hardening method for tissue culture seedlings of rhododendron lapponicum. The method comprises acclimation of tissue culture rooting seedlings in a bottle, transplantation, environment control after transplantation, fertilization management and pest control, wherein the environment control is implemented as follows: a small arch shed is put up below a sunshade net arch structure set up in a large shed, the top surface of the small arch shed is covered with a sunshade net, and a hole tray is put in the small arch shed; the humidity of the small arch shed is kept at 80%-85%, the temperature in the daytime is kept 25 DEG C-30 DEG C, the temperature at night is kept not lower than 5 DEG C, and moisture is controlled; fertilization management and pest control are implemented as follows: after the tissue culture seedlings are transplanted to the hole tray for 30 days, a leaf fertilizer is applied once every two weeks, chlorothalonil or carbendazim is sprayed once every 15 days and sprayed for 3-4 times in total, and imidacloprid is sprayed once every week at the initial stage of aphids in spring and sprayed for 3-4 times in total. By means of the method, the transition time of the tissue culture seedlings of the rhododendron lapponicum is shortened, the survival rate of hardened seedlings is up to 90% or higher, the seedlings grow healthy and strong, a standard seedling hardening technical scheme is provided, and the method is suitable for industrial seedling hardening.

Description

technical field [0001] The invention relates to rapid hardening of alpine rhododendron tissue cultured seedlings, belonging to the technical field of plant propagation. Background technique [0002] Alpine Rhododendron is the general term for the evergreen Rhododendron and its new horticultural varieties hybridized over hundreds of years in the Rhododendron genus. It is named because most of the species grow in high-altitude areas above 1,000 meters above sea level. It has a variety of flower colors and flower shapes, including single and double petals, and the flower colors are red, yellow, purple, white, etc. and various transitional colors. Its flowers have the characteristics of huge inflorescences, clusters of flowers, and beautiful flowers and leaves, and are loved by people. It has not only become a high-end Chinese New Year flower in China, but also a high-end landscaping plant in the world, with broad market development prospects. At present, the alpine Rhododendro...

Claims

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Application Information

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IPC IPC(8): A01G31/00A01G13/02
CPCA01G13/0206A01G13/0231A01G31/00
Inventor 陆琳李世峰王继华解玮佳彭绿春宋杰李树发蔡艳飞张露
Owner FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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