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Bud stripping and sterilizing method of Rhododendron lapponicum explants

A disinfection method and explant technology, applied in the field of plant tissue culture, can solve the problems of high disinfection pollution rate and death rate, hindering the plant regeneration system, etc., and achieve the effects of good disinfection effect, reduction of use time, and reduction of injury.

Active Publication Date: 2017-12-22
FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the bottleneck problem that the alpine rhododendron explants whose body surface is densely covered with downy hairs or / and glands have high disinfection pollution rate and mortality rate and hinder the establishment of its plant regeneration system, the invention provides a bud-stripping disinfection of rhododendron explants method

Method used

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  • Bud stripping and sterilizing method of Rhododendron lapponicum explants
  • Bud stripping and sterilizing method of Rhododendron lapponicum explants
  • Bud stripping and sterilizing method of Rhododendron lapponicum explants

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Effect test

Embodiment 1

[0022] Embodiment 1 The method of the present invention debuds and sterilizes the explants of Rhododendron lantana

[0023] (1) Pretreatment of budding branches

[0024] In April, select the branches of Rhododendron lantana with strong growth and full side buds, keep the branches to grow on the mother plant, and only remove the top leaf buds and / or flower buds on the branch (that is, after removing the leaf buds and buds on the top of the Rhododendron lantana branch). / or after the flower buds at the top of the branch of Rhododendron lantana, the branch of Rhododendron lantana continues to grow on its mother plant), so as to promote the germination of leaf buds at the top of the branch and / or the germination of lateral buds at the petiole (see figure 1 , figure 2 ).

[0025] (2) Picking buds and stripping buds for the first time

[0026] Treat that the pretreated branch of Rhododendron lantana in step (1) grows on its mother plant for 15 days, and the top leaf bud of the ...

Embodiment 2

[0031] Embodiment 2 The method of the present invention is to the debudding and disinfection of Rhododendron pachyphyllae explants

[0032] Except that the following measures are different in Example 2, other measures are the same as those in Example 1, and will not be repeated.

[0033] (1) Pretreatment of budding branches

[0034] The pretreated shoots were the shoots of Rhododendron pachyphylla.

[0035] (2) Picking buds and stripping buds for the first time

[0036] After the pretreated branch of Rhododendron pachyphylla in step (1) grows on its mother plant for 25 days, the buds are taken and the first bud removal is carried out according to the operation of step (2) of Example 1.

[0037] (3) Germ disinfection

[0038] The time of immersion and disinfection in 0.1% mercuric chloride solution was 12min.

[0039] (4) The second bud removal and inoculation

[0040] Cut off 0.2-0.5mm stem section at the base of the bud body to obtain the explants of Rhododendron pachyph...

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Abstract

The invention discloses a bud stripping and sterilizing method of Rhododendron lapponicum explants. The method mainly includes: removing the top leaf bud and flower bud of a branch for bud taking to promote the germination of lateral buds at the top leaf bud and the petiole of the branch; after the branch grows on a stock plant for 10-30 days and the lateral buds at the top leaf bud and the petiole germinate and when leaves do not completely spread, sterilizing the leaf bud and the lateral buds, and performing bud stripping once before and after the sterilizing to obtain the bud-stripped and sterilized explants, to be more specific, stripping 1-2 layers leaves, from outside to inside, of a bract and the buds. The bud stripping and sterilizing method has the advantages that the tissue culture pollution rate of the bud-stripped and sterilized explants is not higher than 30%, the bud induction survival rate of the bud-stripped and sterilized explants is higher than 63%, the technical bottleneck that the explants of Rhododendron lapponicum whose surface is densely covered by villi and / or glands are difficult to sterilize during the building of the plant regeneration system of the Rhododendron lapponicum is broken through, the building speed of the plant regeneration system of the Rhododendron lapponicum is increased, and technical support is provided for Rhododendron fundamental researches, seedling industrial production and protection of rare and endangered species.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for sterilizing Rhododendron alpine explants whose body surfaces are densely covered with villi or / and glands, and can be widely used in basic research on Rhododendron and plants in the factory production of seedlings. The explant disinfection link established by the regeneration system. Background technique [0002] The establishment of a high-efficiency plant regeneration system is the basic research on genetic engineering breeding of Rhododendron, the stable propagation of hybrid offspring, as well as the technical basis for the industrial production of Rhododendron seedlings and the conservation of rare and endangered species. The above applications all require that vegetative organs (such as buds and stem segments) be used as explants in the regeneration system of Rhododendron plants, so as to maintain the consistency of the reproductive off...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/005
Inventor 彭绿春宋杰李世峰解玮佳李树发蔡艳飞张露
Owner FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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