Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Improved method for extracting total DNA (deoxyribonucleic acid) from polysaccharide and polyphenol plant Rhododendron lapponicum

An extraction method, Rhododendron technology, applied in the field of plant life science, can solve the problems of poor DNA integrity, many impurities, and low purity, and achieve the effect of improving DNA yield, high purity, and good repeatability

Inactive Publication Date: 2016-06-29
FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
View PDF5 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to solve the technical problems such as more impurities, lower purity and poor DNA integrity in the DNA extraction of Rhododendron alpine by the conventional CTAB method, the present invention provides an improved method for extracting the total DNA of rhododendron alpine polysaccharides and polyphenols

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Improved method for extracting total DNA (deoxyribonucleic acid) from polysaccharide and polyphenol plant Rhododendron lapponicum
  • Improved method for extracting total DNA (deoxyribonucleic acid) from polysaccharide and polyphenol plant Rhododendron lapponicum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Rhododendron lantana (sample 1), Rhododendron yunnanensis (sample 2), Rhododendron chinensis (sample 3), Rhododendron grandiflora (sample 4) and Rhododendron wide cup (sample 5) were used as experimental materials. , extract the total DNA of Rhododendron alpine dry leaves with the following improved 4×CTAB method, that is, the method of the present invention. Materials such as the five different species of Rhododendron alpine and various reagents used in the examples are commercially available.

[0031] (1) Material grinding

[0032] Weigh 100 mg of Rhododendron alpine dry leaves and place them in a mortar with liquid nitrogen added in advance to grind them into powder; since the collected fresh Rhododendron alpine leaves need to be immediately ground in liquid nitrogen to prevent loss of DNA, the inventors used fresh Rhododendron alpine leaves and dried leaves as experimental materials, and compared the DNA extraction effects of 2 × CTAB, 4 × CTAB and improved 4 × CTA...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an improved method for extracting total DNA (deoxyribonucleic acid) from polysaccharide and polyphenol plant Rhododendron lapponicum. A polysaccharide removal treatment step is added to the method, dried Rhododendron lapponicum leaves are ground into powder with liquid nitrogen, then 1 mL of Buffer I is added, and most secondary metabolites such as polysaccharides, polyphenols and the like in a plant cell homogenate are washed through ice bath and centrifugation; a cell lysis solution adopts 4*CTAB (cetyltrimethylammonium bromide); cell lysis mixed liquor is not extracted directly during DNA extraction, but a supernatant obtained from separation after high-speed centrifugation of the cell lysis mixed liquor is extracted; a Rnase A reagent is added to a supernatant obtained through first extraction, the obtained mixture is treated at the room temperature for 20 min, and then second conventional extraction is performed; during washing of DNA precipitates, the arranging direction of a centrifuge tube is changed to the opposite for next washing, and the high-speed centrifugation time is prolonged to 5 min. The method has the advantages of high yield, high purity and good repeatability and can be applied to downstream experiments of molecular biology.

Description

technical field [0001] The invention relates to the technical field of plant life science, in particular to an improved high-efficiency (high yield and high purity) DNA extraction method for the polysaccharide polyphenol plant rhododendron alpine. Background technique [0002] Rhododendron L. is a world-renowned garden ornamental plant, one of the top ten famous flowers in my country and one of the eight famous flowers in Yunnan Province, China. Alpine Rhododendron is a general term for some original species of evergreen broad-leaved Rhododendron and its hybrid offspring. Because of its beautiful plant shape, large and colorful flowers, and evergreen seasons, it has gradually become a rookie in my country's New Year's Eve flowers and high-end landscaping. my country has more than 320 species of rhododendrons, accounting for 1 / 3 of the world's rhododendrons. The southwest and adjacent eastern Himalayas are the distribution centers and origins of the evergreen subgenus Rhodode...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/10
CPCC12N15/1003
Inventor 张露李世峰蔡艳飞解玮佳宋杰彭绿春李树发陆琳
Owner FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com