42results about How to "Add processing steps" patented technology

The invention belongs to the field of microorganism detection and relates to a method for quantitative detection of living bacteria based on FISH technology. Before the implementation of the conventional fluorescence in situ hybridization method, the method adds the pretreatment step of PMA nucleic acid dye; during the PMA treatment process, the nucleic acid in the dead bacteria cells is covalently combined with PMA, and it emits orange under the blue light excitation of the fluorescence microscope Fluorescence, so that it can be known that the bacteria with orange fluorescence are dead bacteria in the original system, and the rRNA in the living bacteria cells is combined with the fluorescent probe in the hybridization step, and the fluorescent probe emits green fluorescence under the blue light excitation of the fluorescence microscope, so that the green fluorescence can be known Bacteria are living bacteria in the original system. The invention realizes the effective distinction of dead and alive bacteria in the sample system by improving the fluorescence in situ hybridization technology, that is, adding the step of PMA dye treatment, and constructs a set of methods that can quickly quantify the active bacteria in the sample. This method has strong universality for bacterial quantification.

The present invention discloses a camphor molecular imprinted polymer, and a preparation method and application of a monolithic column and a packed column thereof. The preparation method includes dissolving a template molecule camphor in a pore-foaming agent, adding a functional monomer, a crosslinker and an initiator, mixing, treating by ultrasound in an ice-water bath, feeding nitrogen, injecting the reactant into a stainless steel chromatographic column and a container, sealing, placing in an oven of 50-60 DEG C to react for 12-24h, and initiating by heat to obtain a molecular imprinted polymer monolith column and the polymer. The polymer is ground, sieved and filled in a chromatographic column to get a molecular imprinted polymer packed column. The packed column and the monolithic column are connected with a liquid chromatography pump to remove the template molecules. Finally the chromatographic and the monolithic column with good separation effects are obtained. The method is good in molecular recognition. The molecular imprinted polymer provided by the invention as a liquid chromatographic column filler can realize separation, enrichment and purification for camphor in essential oil.

The invention discloses a method for determining five inorganic positive ions in a tobacco gene editing material. An apparatus is used, which comprises a sample extraction bottle, an outer cannula (1), an inner cannula (2), a liquid-guiding pipe (4), and a sieve plate (3) which can be one or more and positioned in a lower opening of the inner cannula (2). The method comprises the following steps:1) tobacco leaf extraction; 2) filtering and transferring; 3) analysis for making a chromatogram; 4) acquisition of a peak area of the standard chromatogram to obtain the inorganic positive ions of the tobacco gene editing material. The determination method of the present invention is characterized in that an on-line solid phase extraction purifying column is added before first usage of an ion chromatographic column, the processing is simple and convenient, the effect is good, the determination method has the advantages of accurate quantification, good repeatability, and low detecting limit, and can effectively realize the high flux analysis determination of the inorganic positive ions in tobacco gene editing material.

The invention discloses a relay communication system and channel sorting method based on channel sorting and network coding, which is characterized in that in the data uplink stage, the channel sorting module of the relay node receives K received signals and performs channel sorting to obtain sorted received signals. After the signal is sent to the signal quantization module; the signal quantization module performs channel quantization processing on the sorted received signal and then sends it to the signal network coding module for network coding, and finally estimates the number of the sorted first data packet and the second data packet Network encoding format. The invention can effectively reduce the symbol error rate of communication, increase the data rate and system capacity, and reduce the dependence of information transmission on the quality of the wireless channel, especially the performance improvement under low signal-to-noise ratio is more obvious.