A method for evaluating the safety of meat products using zebrafish

Abstract

The invention provides a method for evaluating the safety of meat products by using zebrafish. Firstly, the meat product is treated with bionic digestion and extraction to obtain the meat sample extract, and then the processing time of the meat sample extract is determined to be 24 hours. The treatment concentration was 1.4-5.6 mg / mL to construct a zebrafish model and evaluate the safety of meat products. The zebrafish model constructed by the present invention and the method for evaluating the safety of meat products have high detection accuracy, the detection method is scientific and reasonable, and the short modeling cycle greatly reduces the detection cost, which provides a basis for popularizing and popularizing the detection method to the general public, enterprises and governments. Realistic feasibility.

Description

technical field [0001] The invention relates to the technical field of safety evaluation of meat products, in particular to a method for evaluating the safety of meat products by using zebrafish. Background technique [0002] Meat products are an indispensable consumer food in people's daily life, such as chicken, duck, fish, pig, cattle, sheep and other animal meat products, most of which come from farming. With the increasing demand of residents for daily products, driven by economic interests, the abuse of veterinary drugs (including antibiotics and their semi-synthetic products, chemically synthesized drugs and other additives) in the breeding industry has also intensified, resulting in the use of veterinary drugs in animal-derived foods. The residual content exceeds the standard; some unscrupulous farmers even use various adulteration methods to make "synthetic meat" to make huge profits. The risk of eating is higher. All kinds of strange phenomena have led to increas...

AI Technical Summary

Problems solved by technology

The current food safety inspection items are basically physical and chemical inspections and biochemical inspections. The detection indicators are mechanized and poorly related to organisms, and have defects that cannot be ignored: 1) Due to the interaction between various chemicals, the residues may also be low Chemicals that are lower than the standard value are harmful to the human body, so whether the residual amount of harmful substances below the standard value is absolutely safe for the human body, there is uncertainty

2) The existing detection system only detects known harmful substances listed in the testing standards, and cannot detect harmful substances that are not listed in the testing standards or unknown. We cannot judge and prevent possible harm caused by food poisoning unknown substances

[0007] 1) Unable to detect the synergistic toxicity of different chemical substances to the human body

Due to the unknown physical and chemical properties of different chemicals, there is uncertainty about whether they can produce synergistic effect or synergistic reduction when interacting; it is more difficult to judge the synergistic toxicity of the sample to be tested than the toxicity of a single toxic substance

[0008] 2) Unknown additives cannot be detected, only known chemical substances can be detected

If there are analogues, derivatives of known chemical substances and their metabolites in the human body as unknown additives, it is difficult to detect

[0009] 3) There are many types of toxic substances, considering cost factors such as time, manpower, and material resources, it is impossible to cover everything and achieve full inspection

[0013] To sum up, how to provide a suitable method to evaluate the safety of meat products with zebrafish, provide safety guarantee for people to eat meat products with confidence, provide quality control guarantee for meat products produced by suppliers with confidence, and reassure regulatory agencies Supervising the safety of meat products in the production and distribution links and providing technical support is a technical problem that has not been solved by those skilled in the art; it is also a major technical challenge to establish testing standards for meat products using biological means

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