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A method for the determination of inorganic cations in tobacco gene editing materials

A technology of gene editing and determination method, applied in the field of analytical chemistry, can solve the problem that conventional methods cannot meet the needs, and achieve the effects of improving sample analysis efficiency, good compatibility, and shortening analysis time.

Active Publication Date: 2020-07-17
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

To carry out chemical analysis and evaluation on these massive materials, conventional methods are difficult to meet the needs

Method used

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  • A method for the determination of inorganic cations in tobacco gene editing materials
  • A method for the determination of inorganic cations in tobacco gene editing materials
  • A method for the determination of inorganic cations in tobacco gene editing materials

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Take 4 kinds of finished tobacco leaves as investigation samples, and measure cationic content wherein, comprise the steps:

[0040] ①Sample pretreatment: Select 28 finished tobacco leaves currently in use as the investigation objects, dry them in an oven at 40°C, then crush them and pass them through a 40-mesh sieve; accurately weigh 0.2g of the sample powder into the outer tube, add 50mL of 5wt % hydrochloric acid aqueous solution as the extraction agent, and then, fix the inner casing of the extraction bottle on the mouth of the outer casing with a sealing ring. At this time, the extraction solution enters the catheter through the filter sieve and flows out from the catheter to achieve the effect of filtering the sample. Collect 1-1.5mL of the extract from the bend of the catheter to the outlet, and perform on-line purified ion chromatography analysis.

[0041] ②Ion chromatography analysis: Add an online solid phase extraction purification column before the ion chro...

Embodiment 2

[0045] Tobacco seedlings from 5 material samples from gene editing and breeding factories were selected as the research objects, and the samples were freeze-dried at -80°C. Sample processing and ion chromatographic analysis are the same as in Example 1. The measurement results show that: (a) the potassium content of one material is 0.285wt%, which is far lower than the average value of 1.64wt% of normal tobacco seedlings. Potassium absorption disorder. After continuous cultivation, the leaf margins turn yellow, and then turn brown, scorched like burning; brown spots or plaques appear on the leaves. (b) The potassium content of the two materials is 0.126wt%, which is far lower than the average value of 0.537wt% of normal tobacco seedlings. Magnesium absorption disorder, after continued cultivation, leaf chlorosis appeared, and the chlorosis spread from the leaf tip and leaf edge to the leaf base and middle, and the leaves formed reticular veins. (c) Absorption of inorganic ca...

Embodiment 3

[0047] Taking 5 kinds of finished tobacco leaves of Baijia tobacco as investigation samples, the content of 5 kinds of inorganic cations was determined. The measurement steps were the same as in Example 1. The content of inorganic cations in the samples was consistent with the range of inorganic cations in high-quality Baijia tobacco.

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Abstract

The invention discloses a method for determining five inorganic positive ions in a tobacco gene editing material. An apparatus is used, which comprises a sample extraction bottle, an outer cannula (1), an inner cannula (2), a liquid-guiding pipe (4), and a sieve plate (3) which can be one or more and positioned in a lower opening of the inner cannula (2). The method comprises the following steps:1) tobacco leaf extraction; 2) filtering and transferring; 3) analysis for making a chromatogram; 4) acquisition of a peak area of the standard chromatogram to obtain the inorganic positive ions of the tobacco gene editing material. The determination method of the present invention is characterized in that an on-line solid phase extraction purifying column is added before first usage of an ion chromatographic column, the processing is simple and convenient, the effect is good, the determination method has the advantages of accurate quantification, good repeatability, and low detecting limit, and can effectively realize the high flux analysis determination of the inorganic positive ions in tobacco gene editing material.

Description

technical field [0001] The invention relates to the technical field of analytical chemistry, in particular to a method for determining inorganic cations in tobacco gene editing materials. Background technique [0002] Potassium, sodium, calcium, magnesium and ammonium are essential nutrients for tobacco growth and development, and the content of these elements in finished tobacco leaves is closely related to the quality of tobacco leaves. Therefore, the accurate determination of potassium, sodium, calcium, magnesium and ammonium in tobacco is of great significance to the growth, development and quality evaluation of tobacco. At present, the determination of potassium, sodium, calcium and magnesium generally adopts continuous flow analysis, atomic absorption spectrometry, near-infrared spectroscopy, and atomic emission spectrometry; the determination of ammonia generally adopts continuous flow analysis, ion chromatography and ion selective electrode method. Ion chromatograph...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/062
Inventor 陈章玉李晶李雪梅米其利陈建华耿永勤张承明缪恩铭杨光宇田丽梅向明蒋次清
Owner CHINA TOBACCO YUNNAN IND
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