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A rhododendron alpine mycorrhizal fungus tr11 and its application

A technology of mycorrhizal fungus and Rhododendron, applied in the field of microbiology, can solve the problems of rapid cultivation of multiple varieties of Rhododendron, achieve enhanced ability and adaptability to the environment, improve vegetative growth, improve quality and price

Active Publication Date: 2020-05-01
LUDONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although the symbiotic mycorrhizal fungus used in the above literature has the characteristics of promoting the growth of Rhododendron grandiflora, it still cannot meet the relevant requirements for rapid cultivation of multiple varieties of Rhododendron.

Method used

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  • A rhododendron alpine mycorrhizal fungus tr11 and its application
  • A rhododendron alpine mycorrhizal fungus tr11 and its application

Examples

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Embodiment 1

[0031] A strain of Rhododendron alpine mycorrhizal fungus (Ascomycota sp.) TR11 was deposited in the General Microorganism Center (CGMCC) of the China Committee for the Collection of Microbial Cultures on July 26, 2017, and the preservation number is: CGMCC No.14360.

[0032] The characteristics of the colony of this fungus are: the front is light brown, the back is dark brown, the hyphae are fluffy, the growth rate is 4mm / d, and it is classified as Ascomycete.

[0033] The above-mentioned rhododendron alpine mycorrhizal fungus is screened by the following method:

[0034] The root systems of three kinds of rhododendrons were collected respectively from Kunming Botanical Garden, Dali Cangshan, Lijiang Yulong Snow Mountain and Tibet Linzhi, which are the original places of alpine rhododendron. Rinse with water 7 to 8 times, and then isolate the fungi from the roots. The medium is potato dextrose medium (PDA); after purification, co-cultivation and secondary isolation and cultur...

Embodiment 2

[0042]The bacterial particles in the liquid medium prepared in Example 1 were added to the medium planted with aseptic seedlings of Rhododendron maxima to carry out the synthetic mycorrhizal experiment, and vermiculite plus peat soil (volume ratio 1:3) was used as co-cultivation matrix. Select the healthy and plump seeds of Rhododendron maxima, wash them with sterile water first, then use 0.1% mercuric chloride solution for surface disinfection for 4 minutes, then rinse them with sterile water for 7-8 times, put them into sterilized petri dishes for germination ; Pick a small amount of mycelia from the purified strains and add them to the liquid PDA medium, add glass beads with a diameter of 3 to 4 mm to gather the mycelia, put them in a shaker, and the speed is 130 to 150r / min, cultured in the dark at 23°C for 15 days to obtain bacterial particles for inoculation; after the aseptic seedlings germinated and grew two true leaves, transplant them to soil with vermiculite and pe...

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Abstract

The invention relates to a rhododendron alpine mycorrhizal fungus (Ascomycota sp.) TR11 and its application. A rhododendron alpinus mycorrhizal fungus TR11 was deposited in the General Microorganism Center of China Committee for Microbial Culture Collection on July 26, 2017, address: Institute of Microbiology, Chinese Academy of Sciences, No. 1, Yard 1, Beichen West Road, Chaoyang District, Beijing, deposit number For: CGMCC No.14360. The invention also relates to the cultivation method and application of the bacterial strain. Rhododendron alpine mycorrhizal fungus TR11 described in the present invention can symbiosis with various alpine rhododendrons including Rhododendron maxima, and promote their growth. Compared with existing known symbiotic mycorrhizal fungi, which can only promote the growth of a single species of Rhododendron, it has obvious advantages. Versatility.

Description

technical field [0001] The invention relates to a rhododendron alpine mycorrhizal fungus TR11 and its application, belonging to the technical field of microbes. Background technique [0002] Alpine rhododendrons have various colors and beautiful trees. As one of the members, the great white rhododendron (Rhododendron decorum) also has high ornamental and edible value. Rhododendron is a fibrous root system, and it is very easy to affect the absorption of water and nutrients due to poor root system during the cultivation process, affecting vegetative growth and plant type, resulting in a decline in quality. But as the rhododendron grows, it forms a mutualistic symbiont with the fungus, called mycorrhizae. Mycorrhizae can promote the absorption of water and nutrients by rhododendron, thereby enhancing the competitiveness and tolerance of plants, so that plants can still survive under harsh conditions. In order to improve the success rate of rhododendron introduction and domes...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01G18/10A01G22/60C12R1/645
CPCA01G18/10C12N1/14C12N1/145C12R2001/645
Inventor 田伟程显好张长芹张敬丽邹广众
Owner LUDONG UNIVERSITY
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