Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of tissue culture method of sunshine holly

A technique for tissue culture and holly candidum, which is applied in the field of tissue culture of holly candidum, can solve the problems of insufficiency, small reproduction coefficient, slow speed, etc., and achieves the effects of improving efficiency, short culturing time and simple formula.

Active Publication Date: 2020-04-24
SICHUAN COLORLINK CO LTD
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Conventional cultivation methods of Sunshine Narrow Crown Ilex include: semi-hard branch cutting method and grafting propagation method, semi-hard branch cutting method and graft propagation method exist, the reproduction coefficient is small, and the speed is slow, which cannot meet the market demand for Sunshine Narrow Crown Ilex

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of tissue culture method of sunshine holly
  • A kind of tissue culture method of sunshine holly
  • A kind of tissue culture method of sunshine holly

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A method for culturing tissue of Ilex chinensis in sunshine, comprising the following steps:

[0030] Pretreatment: Take the terminal buds or axillary bud stems of the annual Sunshine Ilex narrow crown, on a sterile operating table, first treat it with 75% alcohol for 10 seconds, wash it with sterile water for 1~3 times, and then treat it with 10% sodium hypochlorite solution for 8~ 12min, wash with sterile water 4 to 5 times, and dry to obtain sterilized explants;

[0031] Primary culture: get the sterilized explants and inoculate them in the primary medium for primary culture, such as figure 1 , to obtain axillary buds; the primary medium comprises: NN69 basic medium supplemented with 1.0mg / LCPPU, 0.01mg / LTDZ, 30g / L sucrose, and 6.5g / L agar; the pH of the primary medium is 5.8 to 6.2;

[0032] Proliferation culture: get the axillary buds and inoculate them in the proliferation medium to carry out proliferation culture, such as figure 2 , to obtain strong seedlings;...

Embodiment 2

[0038] A method for culturing tissue of Ilex chinensis in sunshine, comprising the following steps:

[0039] Pretreatment: Take the terminal buds or axillary bud stems of annual Sunshine Ilex narrow crown, on a sterile operating table, first treat with 75% alcohol for 10 seconds, wash with sterile water for 1 to 3 times, and then treat with 10% sodium hypochlorite solution for 8~ 12min, wash with sterile water 4 to 5 times, and dry to obtain sterilized explants;

[0040] Primary culture: take the sterilized explants and inoculate them in the primary medium for primary culture to obtain axillary buds; the primary medium includes: NN69 basic medium, supplemented with 0.5mg / LCPPU, 0.05mg / LTDZ, 30g / L sucrose, 6.5g / L agar; the pH value of the primary culture medium is 5.8~6.2;

[0041]Proliferation culture: get the axillary buds and inoculate them in the proliferation medium to carry out the proliferation culture to obtain the proliferation seedlings; the proliferation medium comp...

Embodiment 3

[0046] A method for culturing tissue of Ilex chinensis in sunshine, comprising the following steps:

[0047] Pretreatment: Take the terminal buds or axillary bud stems of annual Sunshine Ilex narrow crown, on a sterile operating table, first treat with 75% alcohol for 10 seconds, wash with sterile water for 1 to 3 times, and then treat with 10% sodium hypochlorite solution for 8~ 12min, wash with sterile water 4 to 5 times, and dry to obtain sterilized explants;

[0048] Primary culture: take the sterilized explants and inoculate them in the primary medium for primary culture to obtain axillary buds; the primary medium includes: NN69 basic medium supplemented with 2.0mg / LCPPU, 0.1mg / LTDZ, 30g / L sucrose, 6.5g / L agar; the pH value of the primary culture medium is 5.8~6.2;

[0049] Proliferation culture: get the axillary buds and inoculate them in the proliferation medium to carry out the proliferation culture to obtain the proliferation seedlings; the proliferation medium compr...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a tissue culture method for ilex attenuata 'Sunny Foster'. The method includes the following steps of pretreatment: taking stems of terminal buds or axillary buds of the ilex attenuata 'Sunny Foster', and conducting disinfection and sterilization treatment to obtain a disinfected and sterilized explant; primary culture: inoculating the disinfected and sterilized explant into a primary culture medium for primary culture to obtain axillary buds; proliferation culture: inoculating the axillary buds into a proliferation culture medium for proliferation culture to obtain proliferative seedlings; rooting culture: inoculating single plants of the proliferative seedlings into a rooting culture medium for rooting culture to obtain rooting seedlings. The tissue culture methodhas high seedling hardening survival rate, high reproduction coefficient, simple and convenient tissue culture process and short reproduction time, and is not limited by seasons, and the market demand for the ilex attenuata 'Sunny Foster' is met.

Description

technical field [0001] The invention relates to the technical field of tissue culture, in particular to a method for tissue culture of Ilex japonica. Background technique [0002] Sunshine narrow-crown holly is an evergreen shrub of the Ilex genus in the Ilex family. The leaf color changes three times a year. The golden yellow of the new leaves gradually turns dark green, and dark red in winter. It is quite beautiful and is an excellent color block and hedge plant. Conventional cultivation methods of Sunshine Ilex include: semi-hard branch cutting method and grafting propagation method, semi-hard branch cutting method and grafting propagation method exist, the reproduction coefficient is small, and the speed is slow, which cannot meet the market demand for Sunshine Ilex. Contents of the invention [0003] In view of this, the application provides a method for tissue culture of Ilex japonica, the tissue culture method has a high survival rate for hardening seedlings, a high...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 杨良斌田冬梅
Owner SICHUAN COLORLINK CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com