Test-tube Tilapia nilotica breeding method
A technology of Nile tilapia and fish fry, which is applied in fish farming, application, climate change adaptation, etc., can solve the problems of unfavorable large-scale batch production, low reproductive efficiency, and low number of fertilized eggs, and achieve a huge role in promoting and positive meaning, high reproductive efficiency, and high survival rate
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Embodiment 1
[0031] (1) Select mature Nile tilapia parents, that is, choose a male fish whose head, body, tail and fins are pink and obvious, weighing 0.4 kg, and have watery milky white semen when artificially squeezed, and select the genital opening The papillae are short and round, and the reddish female fish weighs 0.2 kg and squeezes several eggs manually. The eggs are uniform in size and light yellow in color.
[0032] (2) Inject male and female fish with the high-efficiency fish oxytocic agent for injection produced by Shanghai Tongyi Biotechnology Co., Ltd. The injection dose for female fish is 60IU, and the injection is completed in one injection; the injection dose for male fish is 60IU, and the injection is completed in one injection. The injection sites were all at the base of the pectoral fin.
[0033] (3) After injecting the female and male broodstock, put them into the oxytocin tank for water stimulation. The water temperature is 25°C. After the effect time reaches 25 hours...
Embodiment 2
[0035] (1) Select mature Nile tilapia parents, that is, choose a male fish whose head, body, tail and fins are pink and obvious, weighing 1 kg, and have watery milky white semen when artificially squeezed, and select genital pore milk The protuberance is short and round, and the reddish female fish weighs 0.6 kg and squeezes several eggs manually. The eggs are uniform in size and light yellow in color.
[0036] (2) Two injections with chorionic gonadotropin (HCG). The injection dose for female fish is 480IU, the first injection dose is 160IU, the second injection is 320IU 8 hours after the first injection, and the injection site is the base of the pectoral fin of the female fish; The second injection is a one-time injection; the injection site is the base of the pectoral fin of the male fish.
[0037] (3) After the female and male broodstock were injected with oxytocin drugs, they were put into the oxytocin pool for stimulation with running water. The temperature of the oxyto...
Embodiment 3
[0039] (1) Select mature Nile tilapia parents, that is, choose a male fish whose head, body, tail and fins are pink and obvious, weighing 1 kg, and have watery milky white semen when artificially squeezed, and select genital pore milk Short and round, reddish female weighs 0.6 kg and squeezes several eggs manually. The eggs are uniform in size and light gray in color.
[0040] (2) Carp pituitary gland (PG) + trophic release hormone analogue (LRH-A 2 ) mixed for one injection. The injection dose for female fish is PG 2.4 mg + LRH-A 2 3 micrograms, one injection, the injection site is the base of the pectoral fin of female fish; the injection volume of male fish is PG 2 mg + LRH-A 2 After 2.5 micrograms are mixed, one injection is completed, and the injection site is the base of the pectoral fin of male fish.
[0041](3) After the female and male broodstock are injected with oxytocin drugs, they are put into the oxytocin tank for water stimulation. The temperature of the ox...
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