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Agipanthus tissue cultivation quick-propagation method

A technique for tissue culture rapid propagation and Agacia japonica is applied in horticultural methods, botanical equipment and methods, plant cells, etc., and can solve the problems of small quantity and inability to multiply seedlings in a short time, and achieves high rooting rate, The effect of high seedling survival rate

Inactive Publication Date: 2007-05-16
上海光兆植物速生技术有限公司
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Problems solved by technology

However, due to the small number, the conventional propagation method is mainly branching, and it is relatively impossible to reproduce a large number of seedlings in a short period of time.

Method used

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Experimental program
Comparison scheme
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Embodiment Construction

[0014] A method for rapid propagation of Agapanthus tissue culture, comprising the following steps: the first step, selecting the chassis of Agapanthus bulbs as explants, and performing aseptic treatment on the explants; the second step, inoculating and bud induction culture in sequence , Differentiation culture, multiplication culture, rooting culture, greenhouse seedling hardening.

[0015] 1. Obtaining explants:

[0016] Remove the outer layer of old leaves and roots of Agapanthus lily, and keep the 15 cm long part of the lower chassis with stems and leaves.

[0017] 2. Aseptic treatment of explants:

[0018] The aseptic treatment refers to putting the Agapanthus bulb chassis into a mercury solution with a concentration of 0.05% for disinfection, then rinsing with tap water, then sterilizing with a bleaching solution with a volume ratio of 1:3 between bleaching water and water, vacuum drying, and finally removing Rinse with sterile water 3 to 4 times on the ultra-clean be...

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Abstract

The invention relates to a method for cultivating lotus, which comprises 1, selecting the steam base of lotus as external plant, disinfecting it; 2, seedling, inducing the germ, increment cultivating, differentially cultivating, rooting, and seeding in large house, while the induce culture medium is MS+6-BA 8.0mg / L. The invention has the advantages that: the product has high root rate and high yield.

Description

technical field [0001] The tissue culture and rapid propagation method of Agapanthus of the present invention relates to a method for propagation of Agapanthus, especially a method for rapid propagation of Agapanthus. Background technique [0002] Agapanthus africanus belongs to the family Amaryllidaceae. It is a perennial herbaceous flower. It is native to South Africa. It is named after its many seeds after flowering. Each flower can bear 20 to 50 flowers. The flowers are blue and purple, and the flowering period is 7~ After 8 months, it can be used as a potted plant, or planted in flower beds, courtyards, etc., beautiful and elegant. Agapanthus is strong in nature, easy to cultivate, not strict in soil requirements, and relatively cold-tolerant, so it is a variety that is very suitable for promotion in landscaping. [0003] Agapanthus was brought back seeds from South Africa in 2003 by a South African returnee. This species is a newly introduced domestic horticultural sp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00C12N5/04A01G1/00
Inventor 苏敏宋学孟肖建梅庞瑞华许雄山章振华王凌健张承妹
Owner 上海光兆植物速生技术有限公司
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