111 results about "Psammosilene tunicoides" patented technology

The invention discloses a psammosilene tunicoides cultivation method, which comprises the following steps of: a, preparing a cultivated land; b, seeding; c, managing field; d, harvesting. Through trial and error, the most appropriate humidity range of seed germination soil for psammosilene tunicoides is obtained, and is strictly controlled between 50 percent and 60 percent until emergence of seedlings; aiming at the characteristics of waterlogging-avoiding of the psammosilene tunicoides, the soil moisture content is strictly controlled between 25 percent and 35 percent after seedling, the qualified seedling percentage (90 percennt-92 percent) and the emergence rate (80 percent-85 percent) are greatly improved, and the yield (480kg / mu-520kg / mu) is improved. The method adopts a direct seeding manner to cultivate the psammosilene tunicoides, and is low in cost, good in product quality since a main root is not easy to diverge, and great in popularization value.

The invention discloses a greenhouse seedling raising method of a Psammosilene tunicoides seed, belonging to the technical field of raising the seedling of a plant seed. The method comprises the following steps: raising the seedling of a Psammosilene tunicoides seed in a greenhouse, maintaining the soil temperature at 20+ / -2 DEG C in the greenhouse, controlling the soil humidity at 50-65% in the greenhouse in a period from the sowing of the Psammosilene tunicoides seed to the growth of two pairs of euphyllas, controlling the soil humidity at 28-38% in the greenhouse in the growing process of the Psammosilene tunicoides seedling from the growth of two pairs of the euphyllas to the growth of three pairs of the euphyllas, and controlling the soil humidity at 15-30% in the greenhouse in the growing process of the Psammosilene tunicoides seedling from the growth of three pairs of the euphyllas to the outplanting of the seedling. By artificially regulating the soil temperature and the appropriate soil humidity for different growth stages of the Psammosilene tunicoides seed based on seedling raising in the greenhouse, the method effectively lowers the morbidity at the seedling stage, improves the germination rate and the survival rate, and raises the strong seedling; and compared with a control sample, the germination rate is improved by 23-26%, the survival rate is improved 12-32%, and the average individual weight is improved by 0.8-1.2g.

The invention discloses an open cultivation method for wild psammosilene tunicoides. The method comprises seed source garden construction, seed selection, seedling raising, propagation transplantation, field management and harvesting measures. A mountain land at the altitude of 1,700 to 2,400 meters is selected. Wild psammosilene tunicoides seeds on a hillside at the altitude of 2,300 to 3,000 meters are selected, a land at the altitude of 2,300 meters is prepared, and is sufficiently applied with a base fertilizer, selected psammosilene tunicoides plants are planted for cultivation, and seeds are collected to realize seed production. Then the produced seeds are subjected to seedling raising on the land under the same environmental condition, and seedlings are transplanted. Land preparation and fertilizer application, planting and seedbed management measures in a seedling raising period and intertilling management measures and the like after transplantation are taken to cultivate psammosilene tunicoides which can be used as a medicinal material.

The invention discloses a quality control method of a capsule preparation for treating a painful swollen joint. The quality control method of the capsule comprises the following steps of property research, identification, inspection and content determination, wherein the identification refers to identifying Yunnan gaultheria, sargentgloryvine stem and psammosilene tunicoides; and the content determination refers to determining the contents of a fumaric acid in the capsule according to high performance liquid chromatography in an appendix VI D of volume one of Chinese pharmacopoeia 2005. The quality control method of a Jingulian capsule preparation for treating the painful swollen joint is provided. The adopted quality control method has the advantages of scientificity, rationality, high accuracy, good reproducibility, comprehensive and effective quality control of the Jingulian capsule, and guaranteed clinical curative effect of the preparation.

The invention relates to a compound external preparation of pseudo-ginseng, that is cataplasm, which is prepared by raw materials of extract (dry extract powder) of pseudo-ginseng, preserved Yunnan Kusnezoff monkshood, paris and psammosilene tunicoides with proper accessories. The compound cataplasm of the invention comprises a backing layer, a body containing ointment and a cover underlayer. The containing ointment body as a main part is formed by extract of pseudo-ginseng, preserved Yunnan Kusnezoff monkshood, paris and psammosilene tunicoides, with accessories such as adhesive, filer, penetration enhancers, humectant, etc., based on a predetermined ratio. The cataplasm has advantages of fast absorption, good moisture retention and ventilation property, and is comfortable for using. In addition, the product of the invention is convenient for using, has little acrimony to skin and has high compliance for patient.

The invention discloses a technical method for rapid sterile seedling propagation of psammosilene tunicoides with axillary buds and immature stems. The method comprises the following steps: shearing psammosilene tunicoides with axillary buds and immature stems as an explant, inducing callus by utilizing the explant, differentiating sterile psammosilene tunicoides seedlings by utilizing the callus, performing optimization selection on types, concentration and matching ratios of different plant growth regulators, screening the optimal matching formula of the growth regulator and additives of psammosilene tunicoides in each period according to application of the different natural plant additives and the influence on psammosilene tunicoides induction and a seedling induction effect, thereby constructing a rapid vegetative propagation system of psammosilene tunicoides with the immature stems. According to aseptic seedlings obtained through vegetative propagation, excellent inheritable characters of maternal plants can be maintained to the greatest degree, and aseptic seedlings are provided. By virtue of the technical method, wild psammosilene tunicoides can be prevented from being subjected to extinctive excavation, the propagation coefficient of psammosilene tunicoides seedlings is greatly improved, and thus the method has important significance for protecting the threatened plant, psammosilene tunicoides, and restoring and protecting biological diversity.

The invention relates to an orally-taken and externally-applied traditional Chinese medicine for treating heorragic trauma, which is a Chinese patent medicine made of traditional Chinese herbs for treating injuries from falls, heorragic trauma, and hyporrhea caused by hyporrheagastric ulcer and duodenal ulcer. The traditional Chinese medicine can be pills or powder made of Paris polyphylla, radix aconiti agrestis, Psammosilene tunicoides and Huaxuedan (blood reduction pill), and can be taken orally or applied externally for injuries from falls and heorragic trauma with obvious treatment effect but no any side effects, and has good treatment effect on hyporrhea caused by hyporrheagastric ulcer, duodenal ulcer and other phlegmonosises.

The invention provides a method for rapidly propagating Psammosilene tunicoides seedlings. The method comprises seed treatment, protocorm induction, protocorm multiplication culture, cluster bud induction, cluster bud multiplication culture and strong seedling culture. The method is characterized in that a protocorm induction culture medium is inoculated with disinfected Psammosilene tunicoides seeds for culture, protocorms with the diameter of 2-3 mm are formed and transferred to a protocorm multiplication culture medium for culture after being cut, a large number of protocorms with the diameter of 2-3 mm are obtained, the protocorms are transferred to a cluster bud induction culture medium for culture, a large number of cluster buds with the length of 1-2 mm are obtained and transferred to a multiplication culture medium for culture, a large number of cluster buds with the length of 10-20 mm are obtained and divided into single buds to be transferred to a strong seedling culture medium for culture, and a large number of detoxified Psammosilene tunicoides seedlings with the length of 80-100 mm are obtained. With the adoption of the method for propagating the Psammosilene tunicoides seedlings, the seedling culture period is short, the propagation coefficient is high, and the seedling quality is good.

The invention discloses a B ultrasonic coupling agent and a preparation technique thereof. The B ultrasonic coupling agent is prepared from 25 parts of propylene glycol, 2 parts of chlorhexidine iodine, 2 parts of hyaluronic acid, 3 parts of sodium carboxymethyl cellulose, 2 parts of sodium dodecyl sulfat, 1 part of vitamin E, 2 parts of carboxymethyl chitosan, 1 part of liquid paraffin, 30 parts of deionized water, 3 parts of aloe oil, 3 parts of prinsepia utilis royle extracts, 2 parts of honeysuckle, 1 part of borneol, 1 part of psammosilene tunicoides and 1 part of polygonum hydropiper. A preparation method comprises preparation of a component A, preparation of a component B, preparation of a component C, preparation of a component D and synthesis of a product. The B ultrasonic coupling agent has the advantages that the B ultrasonic coupling agent is stable in property, has functions of disinfection and sterilization, is free of stimulation for skins and air bubbles, has a remarkable acoustic transmission effect and is clear in ultrasonic imaging and high in speed.

The invention discloses a cultivation method for increasing the psammosilene tunicoides emergence rate. The method includes the steps that psammosilene tunicoides seeds are soaked in normal-temperature water before sowing, the floating immature seeds and impurities are fished out, and the purified full seeds are soaked in the normal-temperature water for 12-24 h, then soaked in a bactericide mixed solution for 15 min to be sterilized, cleaned up and aired for sowing; seedlings are cultivated in a field and a plastic arched shed, in combination of growing environment, illumination, temperature and habits of psammosilene tunicoides, seasonal weather conditions are used, in addition, humidity at different seedling emergence stages of psammosilene tunicoides is controlled manually, the emergence rate can reach 95-100%, and the qualified seedling percentage reaches 90%.

The invention discloses a clarification technology and a quality control method of a medicinal composition having an acne treatment effect. The medicinal composition comprises Underleaf Pearl, Psammosilene tunicoides, Hypericum japonicum Thunb., Machilus wangchiana, Rhododendron primuliflorum Bur. et Franch., Root of Java Campanumoea, Hypericum sampsonii Hance, Lindera caudate, Calyx seu fructus Physalis, Indian kalimeris herb and Matteuccia struthiopteris. The clarification technology is concretely characterized in that medicinal composition extract is clarified by a type II ZTC 1+1 natural clarificant. The quality control method concretely comprises the following steps: carrying out thin-layer chromatography on the Underleaf Pearl, Psammosilene tunicoides, Lindera caudate, Calyx seu fructus Physalis and Indian kalimeris herb, and determining the content of vincetoxicoside, hyperoside, catechin, hypericin, lobetyolin and beta-ecdysone in the medicinal composition through high performance liquid chromatography. The clarification technology and the quality control method have the advantages of less device investment, simple operating process, overcoming of the defects of large alcohol consumption, tedious ethanol recovery, and complex determination process of multiple components in medicines of traditional alcohol precipitation methods, and improvement of the controllability ofthe quality standard of the medicinal composition.

The invention provides a medicament for treating arthralgia, which is prepared from the following raw material drugs in parts by weight: 50 to 100 parts of eucommia, 8.3 to 16.7 parts of zaocys dhumnade, 50 to 100 parts of epimedium herb, 30 to 60 parts of Chinese angelica, 50 to 100 parts of astragalus mongholicus, 20 to 40 parts of ginseng, 50 to 100 parts of mulberry twig, 10 to 20 parts of psammosilene tunicoides, 20 to 40 parts of pseudo-ginseng, 30 to 60 parts of pawpaw, 8 to 16 parts of dog bone glue, 10 to 20 parts of asarum, 30 to 60 parts of teasel root, 30 to 60 parts of hemlock parsley, 20 to 40 parts of monkshood, 33 to 66 parts of sargentg loryvine stem, 40 to 80 parts of large-leaved gentian, 30 to 60 parts of divaricate saposhnikovia root, 50 to 100 parts of photinia serrulata lindl, 30 to 60 parts of clematis root, 30 to 60 parts of radix angelicae pubescentis, and 40 to 80 parts of bighead atractylodes rhizome. According to the medicament, the drug effect is maintained, endangered wildlife can be protected while the curative effect is ensured, potential toxic or side effect can be reduced, and clinical safe administration is guaranteed.

The invention discloses a detection method of a medicinal preparation for treating arthralgia. The detection method is composed of character presentation, identification, inspection and content measurement, wherein the identification is to identify gaultheria yunnanensis, sargentgloryvine stem, psammosilene tunicoides, and Alangium lamarchii Thwaites, and the content measurement is to measure the content of the Alangium lamarchii Thwaites alkali in capsules according to Chinese pharmacopeia 2010 edition Part one appendix VID high performance liquid chromatorgraphy, and to measure the content of the total flavone in the capsules according to Chinese pharmacopeia 2010 edition Part one appendix VA UV-VIS spectrophotometry. The invention provides the detection method of Jingulian capsule preparations for treating painful joints. The employed method is scientific and reasonable, has high accuracy and good reproducibility, can comprehensively and effectively control the quality of the Jingulian capsules, and better ensures the clinic curative effect and safety of the preparation.

The invention discloses a traditional Chinese medicine composition for treating pains in necks, shoulders, waists and legs. The composition is prepared from psammosilene tunicoides, cicada slough, Chinese clematis root, giant knotweed rhizome, obtuseleaf erycibe stem, gentiana macrophylla, Chinese taxillus herb, rhizoma drynariae, rhizoma menispermi, lophatherum herb, caulis clematidis armandii, sappan wood, rhizoma arisaematis, polygala tenuifolia, tall gastrodia tuber, centipedes, radix asparagi, manyprickle acathopanax root, inner membrane of chicken gizzard, galanga galangal seed, grassleaf sweetflag rhizome, fructus amomi, dried tangerine peel and officinal magnolia bark. The traditional Chinese medicine composition is used for preparing clinically acceptable capsules, tablets, pills, granules or oral liquids by adding conventional auxiliary materials according to a conventional process. The medicine composition has the functions of promoting blood circulation to remove blood stasis, dispelling wind and removing obstruction in the meridians, resisting inflammation and easing pains, and has obvious treatment effects on rheumatism, rheumatoid arthritis, hyperostosis, cervical spondylosis, lumbar spondylosis, scapulohumeral periarthritis, lumbar disc herniation, traumatic injuries, lumbar muscle strain or sciatica.

The invention relates to a harmless control method for root rot of Psammosilene tunicoides and belongs to the technical field of traditional Chinese medicine disease control. The harmless control method includes the steps of disease-free seed selection, seed treatment, soil treatment, sowing, field cultivation management and chemical control. The harmless control method for root rot of the Psammosilene tunicoides has the advantages that environmental conditions beneficial to growth and development of the Psammosilene tunicoides and avoidance of root rot are created, disease resistance of plants is improved, average dead plant rate caused by root rot decreases from 80% to 14%, average yield increases by 82.5% as compared with a traditional method, pesticide residues are avoided and economic benefits created by Psammosilene tunicoides planting are enhanced.

The invention relates to a psammosilene tunicoides suspension cell culture system and an establishment method thereof. The establishment method comprises the steps of inducing callus by utilizing psammosilene tunicoides hairy roots as explants, and establishing the suspension cell culture system. On the basis of efficient induction of the hairy roots, the excellent explants is provided for establishing the system by virtue of efficient hairy root induction and culture, the psammosilene tunicoides leaves are treated at the first time by utilizing CuCl2, the leaves are immersed in rooting agrobacterium, the hairy roots can be generated at leaf veins of a leaf stalk and leaf margins in 2 to 4 days, the rooting time is shortened, the rooting can be generated in 3 to 5 days, and the inducing conversion rate is greatly increased and can reach 60 percent or more. The callus is generated by virtue of screening, dedifferentiation and induction, the suspension cell is prepared by virtue of the pretreatment of calcium oxalate, the suspension cell is cultured in an improved culture medium, the secondary metabolism and the biochemical inheritance of the cultured suspension cell are stable, the cell is unlikely to degrade, and a reliable material source and a reliable material foundation can be provided for extracting a natural product from cells.

The invention provides an externally-used Chinese herbal medicine liquor for treating a rheumatoidbone disease. The Chinese herbal medicine liquor is prepared by infusing at least 23 pharmaceuticals including paniculate swallowwort roots 1-100g, radix clematidis 1-100g, fructus psoraleae 1-100g, garden balsam stem 1-100g, Chinese parsnip root 1-100g, chaulmoogratree seed 1-100g, philippine flemingia root 1-100g, wooly datchmanspipe herb 1-100g, celastrus orbiculatus 1-100g, himalayan coralbean bark 1-100g, cochinchina momordicae seed 1-100g, Chinese redbud 1-100g, homalomena occulta 1-100g, entadae stem 1-100g, cyperus rotundus 1-100g, psammosilene tunicoides 1-100g, acorus gramineus 1-100g, short-pedicel aconite root1-100g, angelica sinensis 1-60g, safflower carthamus 1-60g, salvia miltiorrhiza1-60g, radix zanthoxyli 1-60g, corydalis tuber 1-60g, giant knot weed 1-60g and cortex acanthopanacis1-60g. The externally-used Chinese herbal medicine liquor for treating the rheumatoidbone disease has the effects of dispelling the wind and cold-dampness, achieving resuscitation, promoting blood circulation to remove meridian obstruction and alleviate pain, can directly act on the focus position and is easy to permeate, short in treatment course and obvious in effect.

A medicine for treating traumatic injury and bonesetting disclosed in the invention is prepared by the following ingredients: 5-40 weight portions of Herba Chloranthi Serrati, 2.5-20 weight portions of Pendulous Monkshood Root, 5-40 weight portions of Lycopodium cernuum, 4-30 weight portions of black morning glory, 5-40 weight portions of Ligusticum wallichii, 5-40 weight portions of Schisandra propinqua, 5-40 weight portions of Sargentgloryvine Stem, 5-40 weight portions of Helwingia chinensis, 5-30 weight portions of Dysosma, 5-40 weight portions of Psammosilene tunicoides, 5-40 weight portions of keel, and 5-40 weight portions of native copper. The medicine for treating traumatic injury and bonesetting can be prepared into a traditional Chinese poultice for pasting on the wounded place, and can be prepared into a powder which is added in honey to prepare into an adhesive medicine for coating on the wounded place, with dedicated and strong effects of the drug and outstanding curative effect.

The invention discloses a pharmaceutical composition for treating a bone trauma. The pharmaceutical composition for treating the bone trauma is composed of psammosilene tunicoides, euphorbia pekinensis, dendrobium nobile, rhizoma drynariae, white radix achyranthis bidentatae, sambucus williamsii, lycopodium clavatum, sappanwood, cudrania cochinchinensis kudo et masam, musk, tortoise plastron, brasenia peltata tursh, ground beeltle, cornu bubali, yam rhizome, rhizoma kaempferiae, artemisia anomala and calcined natural copper. Conventional auxiliary materials are added into the pharmaceutical composition according to a conventional process, so that clinic acceptant emplastrum or spray or ointment or liniment or tinctures is made. The pharmaceutical composition for treating the bone trauma has the effects of being capable of achieving reunion of bone, muscle and ligament, warming and activating meridian, activating blood and dissolving stasis, and relieving swelling and pain, has good treatment effects for treating bone traumas such as injury of tendon and muscle and fracture, traumatic injury and stagnant blood coagulation, and has no toxic and side effects.

The invention discloses a traditional Chinese medicine composition for treating the visual fatigue of children and a preparation for the traditional Chinese medicine composition. The traditional Chinese medicine composition is prepared from the following raw medicines: dysosma versipellis, psammosilene tunicoides, adder's tongue herb, purslane speedwell herb, alangium chinense harms and aleppo avens. The traditional Chinese medicine composition provided by the invention is non-irritant, has no toxic or side effects, is high in safety, has a radical treatment function, and is relapse-free and capable of effectively treating the visual fatigue of the child; the preparation prepared from the traditional Chinese medicine composition is short in treatment period, good in effect, high in efficiency and relapse-free. According to the traditional Chinese medicine composition and the preparation, which are provided by the invention, the cure rate and the effective rate of treatment for the visual fatigue of the child are higher, the cure rate reaches 96 percent, and the effective rate reaches 100 percent.

The invention discloses an organic planting method for psammosilene tunicoides. The planting method comprises the following steps: 1, selecting land; 2, preparing the land; 3, selecting seeds and treating the seeds; 4, performing sowing; 5, performing thinning and performing transplanting; and 6, performing field management. According to the invention, creatively, flower buds which grow in summers are pruned to promote root growth, the seeds which are ripe in autumns are batched according to harvesting time, the seeds are separately planted through selection, and therefore problems that the psammosilene tunicoides seeds have a bad sowing seedling emergence rate and the fertilization is difficult to uniformly control because the psammosilene tunicoides seeds have inconsistent maturity periods are effectively solved; and flower branches are pruned in the summers, thus no seeds are generated, autumn seeds are cultivated and timing harvest is performed, thus a situation in the prior art that cleaning is difficult because the seeds which are too late to harvest are fallen in field and become weeds is avoided. Through cool and heat alternate seed soaking, the seeds are promoted to germinate, and seed coats are loose and soft, and through slow stirring, the seed coats are broken or break away under conditions that the seeds are not damaged, and the seedling emergence rate of the seeds is greatly improved.

The invention discloses a bedsore paste. The traditional Chinese medicine composition is composed of the following components in parts by weight: lithospermum, ginseng, dog oil, caulis spatholobi, lily, sida acuta, psammosilene tunicoides, garcinia hamburgy hook, willow root, gastrodia elata, polygonum cuspidatum, vitamin C, vitamin B2, radix angelicae, radix achyranthis bidentatae, zaocys dhumnade, lycopodium clavatum, oldenlandia diffusa, houttuynia cordata, chitosan, frankincense, gallnut, radix sophorae flavescentis, raw rheum officinale, patrinia, pseudo-ginseng, garden burnet, angelica sinensis, root of oriental buckthorn, moutan bark and metronidazole. The bedsore paste disclosed by the invention has the functions of clearing away heat and toxic materials, invigorating blood circulation and removing stasis, removing the necrotic tissue and promoting granulation, and dredging channels and collaterals. The bedsore paste disclosed by the invention further has the functions of astringing and relieving pain, invigorating blood circulation and removing stasis and avoiding percolate and infection, and is capable of rapidly controlling infection, remarkable in curative effect in clinical application, unique in curative effect, good in medicine property exertion, low in dosage, convenient to use, simple in production process, convenient to store and suitable for clinical popularization and application.

The invention discloses a membrane laying and close planting method for psammosilene tunicoide. The method comprises the following steps: 1, selection and preparation of land: selecting slopes exposed to the sun for red earth or yellow sand as plantation land, deeply overturning and exposing under the sun; 2, disinfection and germination acceleration of seeds; 3, ridging and membrane laying: making a land with high humidity in a land parcel after soil preparation, wherein the land is 80-100 cm wide and 20-30 cm high; utilizing a mulching film with the width being 120-150 cm and directly lay the film on a neat land surface; 4, sowing; 5, thinning and final singling; 6, carrying out field management; 7, harvesting. The field management comprises tillage and weeding, overwinter management, water and fertilizer management, prevention and control over pests and diseases and picking of flower buds. The membrane laying and close planting method for psammosilene tunicoide has the following beneficial effects: topsoil depth of soil is effectively improved; the diurnal temperature variation is increased; water content of soil is stabilized; rate of change of the soil micro-environment is lowered; rapid growth of psammosilene tunicoide is promoted; the incidence rate of plant diseases and insect pests and psammosilene tunicoide is lowered; a continuous cropping obstacle of psammosilene tunicoide is effectively eliminated; and output and quality of psammosilene tunicoide are improved.

The invention discloses a method for measuring content of an in-blood chemical component-allantoin in a medicinal material-psammosilene tunicoides. The method is a high performance liquid chromatography which takes an allantoin reference substance as the reference and the ratio of acetonitrile or methanol to water as (60-100):(0-40) as the flowing phase. The method selects the in-blood chemical component-allantoin in the medicinal material-psammosilene tunicoides as the index to carry out content measurement; the measuring method is high in precision degree, good in reproducibility, good in stability, high in recovery rate and accurate in measuring result, and the quality of the medicinal material-psammosilene tunicoides can be effectively controlled, so that the safety and effectiveness in clinical medication of the psammosilene tunicoides are ensured.

The invention belongs to the field of traditional Chinese medicines and discloses traditional Chinese medicine tea with an effect of dispelling effects of alcohol and a preparation method of the traditional Chinese medicine tea. The traditional Chinese medicine tea with the effect of dispelling effects of alcohol comprises the following components in parts by weight: 13-18 parts of radix puerariae, 5-10 parts of salvia miltiorrhiza, 3-8 parts of chrysanthemum, 6-12 parts of mung bean, 8-12 parts of waxberry, 6-14 parts of psammosilene tunicoides, 3-10 parts of large-leaved gentian and 7-13 parts of fimbriae orostachys. The preparation method comprises the following steps: performing freeze drying or vacuum drying on each component, grinding the components, and uniformly mixing all the components, thereby obtaining the traditional Chinese medicine tea with the effect of dispelling effects of alcohol.

The invention relates to traditional Chinese medicine for a cure of rheumatoid arthritis. The traditional Chinese medicine comprises medicinal materials including, by weight, 6 parts of Morinda offcinalis, 2 parts of transverse-leaf hypericum monogynum, 1 part of pepper, 8 parts of aspongopus, 8 parts of caulis trachelospermi, 4 parts of little multibanded krait, 0.2 part of Psammosilene tunicoides, 12 parts of Chinese Pyrola Herb, and 9 parts of fructus liquidambaris. Clinical trials prove that the traditional Chinese medicine can safely and effectively cure the rheumatoid arthritis.

The invention relates to a low temperature seedling hardening method for a psammosilene tunicoides tissue culture seedling. The method comprises the steps: transferring psammosilene tunicoides bottled seedling into a seedling hardening culture chamber, and fumigating in a closed manner for 20 min by virtue of potassium permanganate and formaldehyde; culturing the tissue culture seedling for 7 days in an environment with good ventilation at the ambient temperature of 16 to 18 DEG C, the light intensity of 1800 to 2000 Lx and the lighting time of 12 h / d; adjusting the temperature to 11 to 13 DEG C, adjusting the light intensity to 1500 to 1800 Lx, continuously culturing for 14 days, then taking out the psammosilene tunicoides tissue culture seedling by virtue of tweezers, soaking in a 800-time carbendazim disinfectant for 2 h, air-drying, and then transplanting the psammosilene tunicoides tissue culture seedling into a seedling hardening matrix. On the basis that the content of soluble sugar of plants is increased in a low temperature environment, the water content is reduced, the enzyme activity is reduced and air holes are closed, the stress resistance of the plant is finally improved, the rejuvenation period is obviously shortened after the seedling is transplanted, and the probability of the plant infected by root rot and stem rot is reduced, so that the transplanting survival rate is increased to 95 percent or more, and the seedling hardening transplanting survival rate is greatly increased.

The invention discloses Chinese herbal medicinal pills for treating rheumatism and a preparation method for the Chinese herbal medicinal pills. The Chinese herbal medicinal pills are prepared from the following raw materials: short-stem ardisia crenata, white quartz, white perilla leaves, premna ligustroides hemsl roots, trifoliate acanthopanax roots or roots-barks, wet china hypericum, large-leaved cayratia, rhodiola yunnansis, Omei angiopteris, discolor cinquefoil herb, common adenostema herb, hyptis suaveolens, radix ceratostigmae, stems of greyblue pericampylus, caulophyllum robustum, polygonatum, rhizome of dwarf galangal, psammosilene tunicoides, erodium stephanianum willd, salvia plebeia, small-leaved lemmaphyllum, glutinous rice rhizome and roots, grapevine roots, zanthoxylum rhetsoides, herba taxilli, pine pollens, paulownia tomentosa roots, marmot fat and honey. According to the Chinese herbal medicinal pills for treating rheumatism, rheumatism, particularly arthritic rheumatism, can be effectively treated, and the Chinese herbal medicinal pills are convenient to take and free of side effects, and have prominent effects.

The invention provides a saponin compound, which comprises psammosilene tunicoides saponin B and psammosilene tunicoides saponin C which are extracted and separated from traditional Chinese medicine psammosilene tunicoides. The saponin compound is capable of inducing organism to generate Th1 type and Th2 type immune response and inducing the organism to generate stronger cellular immunity and humoral immunity reaction on vaccines as compared with alumina gel adjuvant, thereby being capable of serving as immunologic adjuvant of various vaccines to achieve higher immunity effect. The saponin compound is low in haemolysis, fine in safety, simple and convenient in preparation method, easily controllable in quality, convenient in use, capable of realizing cryopreservation, thereby having higher clinical application value.