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Method for measuring content of in-blood chemical component-allantoin in medicinal material-psammosilene tunicoides

A method of determination and technology of chemical composition, which is applied in the field of content determination of allantoin, a chemical component in Jintiesuo medicinal material, can solve the problems of no thin-layer chromatography identification item, no content determination item, non-compliance, etc.

Inactive Publication Date: 2013-05-22
GUIZHOU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But at present, there is no literature report on the research on the quality control method of Jintiesuo medicinal material
Although Jintiesuo was included in the 2003 edition of "Quality Standards for Traditional Chinese Medicinal Materials and Ethnic Medicinal Materials in Guizhou Province", there are neither TLC identification items nor content determination items in the standard; and in the 2010 edition of "Chinese Pharmacopoeia" also Only the TLC identification item of the control medicinal material
And if there are no strict quality standards and scientific testing methods, it will be difficult to effectively control its internal quality, the safety and effectiveness of medication cannot be guaranteed, and it does not meet the requirements of the international pharmaceutical market, which may seriously restrict the development of my country's traditional Chinese medicine industry.

Method used

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  • Method for measuring content of in-blood chemical component-allantoin in medicinal material-psammosilene tunicoides
  • Method for measuring content of in-blood chemical component-allantoin in medicinal material-psammosilene tunicoides
  • Method for measuring content of in-blood chemical component-allantoin in medicinal material-psammosilene tunicoides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0085] Example 1: The specific method for determining the content of allantoin, the blood-entering chemical component in Jintiesuo medicinal material, is as follows: according to the "Chinese Pharmacopoeia" 2010 edition an appendix VID high-performance liquid chromatography:

[0086] Chromatographic conditions and system suitability test: Amino-bonded silica gel is used as filler; acetonitrile: water = 93:7 is used as mobile phase; flow rate is 1mL min-1; column temperature is 40°C; detection wavelength is 220nm; theoretical plate The number should not be less than 3000;

[0087] Preparation of reference substance solution: Accurately weigh allantoin reference substance, add 20% ethanol to make a solution containing 0.05-0.30mg allantoin per 1mL, and obtain it;

[0088] Preparation of the test solution: take 2 g of Jintiesuo medicinal material powder, accurately weigh it, put it in a conical flask or a conical flask with a stopper, add 30 mL of 20% ethanol, weigh it, extract i...

Embodiment 2

[0090] Example 2: A method for determining the content of allantoin, a chemical component of Jintiesuo medicinal material that enters the blood, specifically: according to the "Chinese Pharmacopoeia" 2010 edition, an appendix VID high-performance liquid chromatography:

[0091] Chromatographic conditions and system suitability test: amino-bonded silica gel was used as filler; acetonitrile: water = 80:20 was used as mobile phase; flow rate was 2 mL min -1 ;The column temperature is 50°C; the detection wavelength is 240nm; the number of theoretical plates should not be less than 3000;

[0092] Preparation of the reference substance solution: Accurately weigh the allantoin reference substance, add 50% ethanol to make a solution containing 0.30mg allantoin per 1mL, and obtain it;

[0093] Preparation of the test solution: Take 5g of Jintiesuo medicinal material powder, accurately weigh it, put it in a stoppered Erlenmeyer flask or a conical flask, add 40mL of 50% ethanol, weigh it...

Embodiment 3

[0095] Example 3: A method for determining the content of allantoin, a chemical component of Jintiesuo medicinal material that enters the blood, specifically: according to the "Chinese Pharmacopoeia" 2010 edition, an appendix VID high-performance liquid chromatography:

[0096] Chromatographic conditions and system suitability test: amino-bonded silica gel was used as filler; acetonitrile: water = 99:1 was used as mobile phase; flow rate was 0.5mL min -1 ;The column temperature is 30°C; the detection wavelength is 210nm; the number of theoretical plates should not be less than 3000;

[0097] Preparation of reference substance solution: Accurately weigh an appropriate amount of allantoin reference substance, add 10% ethanol to make a solution containing 0.05mg allantoin per 1mL, and obtain it;

[0098] Preparation of the test solution: Take 0.5g of Jintiesuo medicinal material powder, weigh it accurately, put it in a stoppered Erlenmeyer flask or a conical flask, add 10mL of 10...

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Abstract

The invention discloses a method for measuring content of an in-blood chemical component-allantoin in a medicinal material-psammosilene tunicoides. The method is a high performance liquid chromatography which takes an allantoin reference substance as the reference and the ratio of acetonitrile or methanol to water as (60-100):(0-40) as the flowing phase. The method selects the in-blood chemical component-allantoin in the medicinal material-psammosilene tunicoides as the index to carry out content measurement; the measuring method is high in precision degree, good in reproducibility, good in stability, high in recovery rate and accurate in measuring result, and the quality of the medicinal material-psammosilene tunicoides can be effectively controlled, so that the safety and effectiveness in clinical medication of the psammosilene tunicoides are ensured.

Description

technical field [0001] The invention relates to a method for determining the content of allantoin, a chemical component in Jintiesuo medicinal material, which belongs to the technical field of quality control of medicinal materials. Background technique [0002] Psammosilene tunicoides (Psammosilene tunicoides), is the dry root of Psammosilene tunicoides, a plant of the genus Psammosilene tunicoides, which is used as medicine with the roots. It is mainly distributed in Guizhou, Yunnan, Sichuan, Tibet and other southwestern regions of my country. It is a unique single-genus plant in southwest China, and it is also a commonly used Chinese herbal medicine in Guizhou. It has been listed in the "Chinese Plant Red Book" as a rare and endangered species. protected plants. Jin Tie Suo has the effects of dispelling blood stasis and relieving pain, stopping bleeding and eliminating carbuncle and discharging pus. [0003] According to literature reports, Jintiesuo medicinal materials ...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
Inventor 周欣龚小见陈华国赵超赵杨
Owner GUIZHOU NORMAL UNIVERSITY
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