A Two-step Transplanting Method of Apple Tissue Culture Seedlings with High Survival Rate

Abstract

The invention belongs to the technical field of apple virus-free seedling factory propagation, and especially relates to an apple tissue culture seedling two-step transplanting method with a high survival rate. The method is characterized by comprising the following steps: (1) rooting culture; (2) indoor domestication; (3) waterweed transplantation; (4) transplantation with a waterweed matrix; and(5) field transplantation. The method has the advantages that the operation and technology are simple, the cost is low, the domestication time is short and only lasts for 4 days, and the domestication time is shortened by 10 to 13 days. After first transplantation, the tissue culture seedling autotrophy root systems grow fast, in the 15th day, the length of more than 90% of tissue culture seedling autotrophy root systems can reach 1.5 cm or more; moreover, waterweed has a good moisturizing performance, the temperature and humidity in a greenhouse are easy to control; the survival rate of transplantation is high, the transplantation survival rate of the apple tissue culture seedling can reach 95.6% or more; the roots are not damaged in two transplantations; for the field transplantation, seedling recovery is not needed, the seedlings grow fast and can be grafted in the current year, the application range of the method is wide, and the method is suitable for most apple species.

Description

technical field [0001] The invention belongs to the technical field of factory breeding of apple seedlings free of viruses, and in particular relates to a method for improving the survival rate of apple tissue cultured seedlings by two-step transplantation. Background technique [0002] As one of the 11 dominant agricultural products planned by the Ministry of Agriculture of my country, apples currently have a total cultivated area of ​​31.5 million mu and a total output of 20.5 million tons. The total area and total output account for 39.8% and 34.7% of the world respectively, and the cultivated area and output rank first in the world. One, is the world's largest apple producer. Apple production plays a pivotal role in promoting my country's agricultural efficiency, farmers' income and agricultural economic development. [0003] Tissue culture technology is the main technology for rapid propagation of horticultural crops. It has the advantages of asexual reproduction, maint...

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Problems solved by technology

[0006] In order to solve the problems of long hardening time before transplanting of apple tissue cultured seedlings in the above prior art, strict requirements on transplanting substrates, and low survival rate, the present invention provides a simple technology, low cost, high survival rate, and robust plant growth. , an apple tissue culture seedling transplanting method suitable for most apple varieties

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