55 results about "Malus x domestica" patented technology

The invention discloses saccharomycete for controlling postharvest penicilliosis of malus domestica and an application of the saccharomycete, and belongs to the technical field of biological control. The saccharomycete is identified as Sporidiobolus pararoseus with the preservation number being CGMCC No. 2.5351. During use, the Sphaerotheca macrophylla is activated, cultured by an NYDB medium for 20 hours and then centrifuged, a thallus is obtained and diluted with sterile water, and the saccharomycete is obtained. With adoption of a saccharomycete suspension and extended penicillium spores for treating the malus domestica, the malus domestica is stored at 20 DEG C for 6 days, and results show that the higher the saccharomycete concentration is, the lower the decay rate is; when the saccharomycete concentration is 1*10<9> cells / mL, the penicilliosis decay rate of the malus domestica is 10.3%, and the penicilliosis decay rate of the control is 100%. Therefore, the Sporidiobolus pararoseus 16 can replace chemical fungicides to control occurrence of the postharvest penicilliosis of the malus domestica, harm of the chemical fungicides to people is avoided, and remarkable economic and social benefits are achieved.

The invention relates to an apple dwarfing self-rooted rootstock SH38 or SH40 large-scale breeding method. The method is suitable for cultivating SH38 or SH40, and specifically comprises the steps oftaking an SH38 or SH40 grafted seedling which is grafted close to the root part and taking malus robusta as a rootstock or a self-rooted seedling as a seed tree, carrying out annual growth branch budnotching, horizontal branch pressing, fixing and soil burying, performing twisting on the new shoot base newly grown from the horizontally pressed branch, carrying out hormone treatment, matrix land-filling, humidity and fertilization management, seedling lifting, and seedling separation to obtain SH38 or SH40 dwarfing self-rooted rootstock. According to the breeding method, the SH38 or SH40 current year-growth semi-lignified shoot does not leave from the parent body to root, and has the advantages that the propagation conditions are mild, the production process is simple, the technical operability and achievability are high, the seedling rate is high, the seedling quality is good, and the like. By adoption of the method of the invention, the apple dwarfing self-rooted rootstock SH38, SH40can realize large-scale and low-cost propagation, thereby obtaining the apple dwarfing self-rooted rootstock SH38 or SH40 with stable traits, neat and consistent tree growth, early flowering and early fruit, fertilizer saving, water saving, land saving and high uniformity of the fruit shape index.

A method of producing trilobatin is disclosed. The method comprising contacting a polypeptide comprising an amino acid sequence at least 90% identical to SEQ ID NO: 8 and having a 4′-O-glycosyltransferase activity with phloretin and UDP-glucose under conditions which allow the formation of trilobatin, thereby producing trilobatin. A method of producing a plant which produces trilobatin, transgenic plant or plant cell and methods of producing transgenic plants are disclosed. Composition comprising trilobatin are also disclosed.

The invention discloses an MdARF3 gene promoter deletion fragment and application thereof in malus plant dwarf detection. The invention provides a method for detecting the stock dwarfing ability of amalus plant. The method comprises the following steps of: detecting whether the genome of a to-be-detected malus plant contains the MdARF3 gene promoter deletion fragment or not, wherein the stock dwarfing ability of the to-be-detected malus plant of which the genome does not contain the MdARF3 gene promoter deletion fragment is higher than the stock dwarfing ability of the to-be-detected malus plant of which the genome contains the MdARF3 gene promoter deletion fragment, and the MdARF3 gene promoter deletion fragment is from -1061st to -992nd site in an upstream direction of A in the initiation codon ATG of the malus plant genome MdARF3 gene. A deletion phenomenon of the MdARF3 gene promoter deletion fragment is positively related to dwarf characters, and a dwarf plant in offspring can bequickly screened by use of the deletion and the existence of the fragment so as to perform an important meaning in a plant dwarfing property field.

A dwarf efficient close-planting cultivation method for apples comprises the following steps: cultivating dwarf Malus baccata nursery stocks, directly grafting target apples to the nursery stocks to obtain dwarf apple nursery stocks, performing high-density field planting on the dwarf apple nursery stocks, forming an efficient close-planting orchard consisting of close-planted apple trees, and the like. According to the method, dwarf interstocks in the prior art are removed, and technical measures such as root control on root systems of the close-planted apple trees, trunk control on trunks, branch control on branches and chemical control on growth are taken in the cultivation process, so that the production cycle is greatly shortened, and the method is quick in fruiting, low in investment, quick in effect and high in economic benefit. The dwarf efficient close-planting cultivation method for apples is simple to operate, easy to implement and low in labor intensity, is particularly suitable for the current situation that the majority of rural areas in China are extremely lack of adult labor force, is deeply welcomed and praised by the masses, can be used for a fixed orchard to achieve good economic benefits, can also be used for a mobile fruit tree base, can be independently sold for mobile fruit trees. can be moved to urban square communities for picking and the like, and is wide in application range.

The invention relates to the field of apple cultivation, and provides a method for cultivating double-virus-free apple seedlings. The method includes the steps: S1, preparing virus-free rootstock seedlings and target variety seedlings; S2, performing rooting, domestication and transplanting culture on the virus-free rootstock seedlings and the target variety seedlings, wherein the virus-free rootstock seedlings are transplanted into a nursery garden in the domestication process, and the virus-free target variety seedlings are transplanted into a nutrition pot in the domestication process; andS3, grafting and culturing the double-virus-free apple seedlings, wherein the step 1 includes pretreatment of explants, stem tip culture, multiple times of high-temperature passivation and multiplication culture, and detoxification detection, and the step 1 is used for simultaneously treating the rootstocks and the target varieties. In conclusion, the rootstocks and the varieties subjected to detoxification treatment are grafted to obtain the required double-virus-free seedlings, so that the situation that the seedlings are non-toxic is effectively achieved, the propagation of soil-borne diseases of soil is inhibited, the resistance to diseases such as soil-borne rhizome rot and root rot is enhanced, thus the use of pesticides can be reduced, and conditions are created for producing greenpollution-free fruits.

The invention discloses an application of a mixed fermentation product of Penicillium vulgaris and Lactobacillus reuteri in relieving successive cropping obstacles of apples, and belongs to the technical field of agricultural microorganisms. Research finds that the mixed fermentation product of Penicillium commune D12 and Lactobacillus reuteri can promote growth of apple seedlings, enhance root activity, improve soil enzyme and rhizopus activity and reduce malondialdehyde content, the number of culturable fungi is remarkably reduced, the number of culturable bacteria is remarkably increased, relative abundance of fusarium causing apple successive cropping obstacles is reduced, the soil microbial community structure is improved to develop in a favorable direction, and the method can be used as an environment-friendly and effective measure for preventing and controlling apple successive cropping obstacles.

The invention discloses an EPSP (5-enolpyruvyl shikimate-3-phosphate) synthase multisite mutant from Malus domestica, and a coding gene and application of the mutant. The mutant has 8 mutation sites, the amino acid sequence of the mutant is as SEQ ID No. 1, and the base sequence of the coding gene of the mutant is as SEQ ID No. 2. Tests show that the EPSP synthase multisite mutant from Malus domestica and the coding gene thereof have high glyphosate resistance, high PEP (phosphoenolpyruvic acid) affinity is kept, and accordingly possibility for using the coding gene for cultivation of transgenic crops is provided.