Endogenous penicillium capable of antagonizing four fusarium fungi and application thereof

A technology of Penicillium oxalicum and microorganisms, applied in the fields of application, fungi, biocides, etc., can solve the problems that have not been reported, and achieve the effect of fast growth

Active Publication Date: 2016-01-20
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies by Shin et al. suggest that Penicillium simplicissimum IFM53375. Penicillium simplicissimum IFM53375. Penicillium simplicissimum IFM53375. The compound has antifungal activity, and the penicillin secreted by Penicillium can degrade phlorizin, but there is no report on the biocontrol of Penicillium in apple continuous cropping obstacles

Method used

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  • Endogenous penicillium capable of antagonizing four fusarium fungi and application thereof
  • Endogenous penicillium capable of antagonizing four fusarium fungi and application thereof
  • Endogenous penicillium capable of antagonizing four fusarium fungi and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Isolation and Purification of Endophytic Penicillium

[0034] The root system of continuous cropping Pingyi sweet tea was subjected to the same surface disinfection and divided into two parts, and one part was cultured without grinding as a control. If no colony grows, it proves that the root surface of the plant has been thoroughly disinfected, thus proving that the isolated fungi are endophytic fungi of the plant rather than airborne or epiphytic bacteria on the surface of the tissue block.

[0035] Under sterile conditions, cut the root system of another continuous cropping Pingyi sweet tea into 1cm-long pieces, soak them in 75% alcohol for 1min, rinse them with sterile water for 2-3 times; Into 0.1% mercuric chloride for 1 min (or in 2% NaClO solution for 0.5 min), rinse with sterile water 4-5 times, and absorb excess water with filter paper. Add sterile water to the root system after absorbing excess water for grinding, and the root system grinding liquid after gr...

Embodiment 2

[0038] Identification of Endophytic Penicillium

[0039] 1. Colony characteristics and morphological characteristics of bacteria

[0040] (1) Colony characteristics

[0041]On the PDA plate, the bacterial colony is flat or nearly flat, with a velvety texture, the mycelium is initially white and then gradually turns dark green, the conidia are easy to fall off, a large number of conidia structures are produced, and the surface of the conidia is dark olive green; Lack of effusion; reverse nearly colorless; lack of soluble pigment. On the CA plate, the colony is flat, there is a circle of white hyphae around the colony, the texture is fluffy, the mycelium is white, the conidia are easy to fall off, the conidia structure is produced in large quantities, and the surface of the conidia is dark green; the exudate is lacking; Reverse nearly colorless; lack of soluble pigment (see figure 1 ).

[0042] (2) Morphological characteristics of bacteria

[0043] Observe the mycelium of t...

Embodiment 3

[0053] Antagonism between endophytic Penicillium oxalicum A1 and four fungi of the genus Fusarium

[0054] 1. Table stand-off test

[0055] The fungi of the genus Beads, Solanum, Oxysporum and Fusarium laminarum used in the experiment were isolated and preserved from the soil of continuous cropping orchards and susceptible apple plants in the Bohai Bay area in 2013 by Professor Yu Jinfeng from the School of Plant Protection of our research group.

[0056] (1) The cultured endophytic Penicillium oxalicum A1 and fungi of the genus Bead, Solanum, Oxysporum and Fusarium exfoliate were respectively punched out with a 0.5cm-diameter punch to form a 0.5cm-diameter cake.

[0057] (2) Introduce endophytic Penicillium oxalicum A1 and four kinds of Fusarium fungus cakes of fungus of the genus Fusarium, beancurd, oxysporum and stratum respectively into PDA medium and corn flour medium (petri dish diameter 9cm) at the same time, endophytic Penicillium oxalicum A1 and Penicillium oxalicum ...

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Abstract

The invention relates to an endogenous penicillium capable of antagonizing four fusarium fungi and application thereof. The endogenous oxalic acid penicillium A1 is obtained through artificial separating and screening from a healthy root system of continuously-cropped malus hupehensis. According to the endogenous penicillium, the growth speed is high, spores are propagated massively, most survival space can be occupied in a short time, the higher antagonizing effect on the main harmful bacteria (such as fusarium solani, fusarium proliferatum, fusarium moniliforme and fusarium oxysporum) which can cause continuous cropping obstacles of apples is achieved, the inhibition rate on the fusarium moniliforme is the highest, the inhibition rate on a corn culture medium is equal to or more than 80%, a certain degradation effect on pholrizin is achieved, and a theoretical basis and a technological base are supplied to biological prevention and control of the continuous cropping obstacles of the applies.

Description

(1) Technical field [0001] The invention relates to an endophytic penicillium antagonizing four kinds of Fusarium fungi and application thereof, belonging to the field of fruit tree cultivation. (2) Background technology [0002] Apple continuous cropping obstacle has a long history, and the etiology is complex. Biological factors related to soil-borne fungi are the main cause of continuous cropping obstacles in most areas. The main genera of pathogenic fungi that have been reported to be related to apple continuous cropping obstacles are Fusarium, Cylindrosporium, Rhizoctonia solani, Phytophthora and Pythium. The pathogenic fungi in the continuous cropping soil of different orchards are different in different regions, and some studies suggest that Fusarium fungi are one of the main pathogens causing apple continuous cropping obstacles. VanSchoor research found that in all continuous cropping apple orchards in South Africa, the soil harmful fungi Fusarium, Cylindrosporium ...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/80
Inventor 尹承苗相立毛志泉张先富王功帅陈学森
Owner SHANDONG AGRICULTURAL UNIVERSITY
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