Application of plant water stress resistance related protein derived from malus sieversiana and coding gene

A technology that encodes genes and plant stress resistance, which is applied in the field of application of proteins related to plant water stress resistance and its coding genes, and can solve problems such as short tree body, decline in apple yield and quality, and weak stress resistance

Active Publication Date: 2020-08-18
CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The intensive cultivation mode of apple dwarf stock is the development direction of the modern apple industry. During the promotion of the dense planting mode of apple short stock, dwarf rootstocks imported from abroad generally have problems such as poor adapt

Method used

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  • Application of plant water stress resistance related protein derived from malus sieversiana and coding gene
  • Application of plant water stress resistance related protein derived from malus sieversiana and coding gene
  • Application of plant water stress resistance related protein derived from malus sieversiana and coding gene

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Embodiment 1

[0090] Example 1. Application of protein MsSCL26 and coding gene in regulating drought resistance of plants

[0091] The amino acid sequence of Xinjiang wild apple protein MsSCL26 is SEQ ID No. 1 in the sequence listing; the nucleotide sequence of the coding strand of the gene encoding protein MsSCL26 (MsSCL26) is SEQ ID No. 2 in the sequence listing. The gene of protein MsSCL26 was cloned from Xinjiang Wild Apple (Malus Sieversii (ledeb.) Roem.)'Xinyuan No. 1', gifted by Professor Liao Kang of Xinjiang Agricultural University in May 2007, preserved in this laboratory, Professor Liao Kang's mailbox: Liaokang01@163.com.

[0092] 1. Analysis of the expression of Xinjiang wild apple MsSCL26 gene induced by water stress

[0093] First, 20% polyethylene glycol 6000 was used to simulate water stress treatments for the rooting seedlings of Xinjiang wild apple tissue culture for 0h, 2h, 4h, 12h, 24h, and the treated plant roots, stems and leaves were quickly frozen with liquid nitrogen and ...

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Abstract

The invention discloses an application of a plant water stress resistance related protein derived from malus sieversiana and a coding gene. The protein is named as MsSCL26 protein, and the protein canbe a protein with an amino acid sequence shown in SEQ ID No.1 in a sequence table. Experiments prove that compared with a plant without the MsSCL26 gene, the MsSCL26 overexpression transgenic plant has remarkable water stress resistance, and it is indicated that the protein MsSCL26 can improve the stress resistance of the plant.

Description

Technical field [0001] The invention relates to the field of biotechnology, in particular to the application of a protein related to plant water stress resistance derived from Xinjiang wild apple and its coding gene. Background technique [0002] Apple ranks first among the four major fruits in the world. As of 2017, my country's total apple cultivation area is 33 million mu, with a total output of 41.39 million tons, accounting for more than 50% of the world's apple cultivation area and output, ranking first in the world ("China Apple Industry Development Report (2017)"). The intensive cultivation model of apple dwarf stock is the development direction of the modern apple industry. During the promotion of the dwarf stock cultivation model of apple dwarf stock, dwarf stocks imported from abroad generally have problems such as poor adaptability and weak stress resistance. Drought stress affects the growth and development of fruit trees. In severe cases, the tree body is short, pr...

Claims

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Application Information

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IPC IPC(8): C12N15/82C07K14/415C12N15/29A01H5/00A01H6/74
CPCC07K14/415C12N15/8271C12N15/8273
Inventor 李天红王彦涛彭翔申晓帅李舰黄学旺朱圣娇
Owner CHINA AGRI UNIV
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