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Saccharomycete for controlling postharvest penicilliosis of malus domestica and application of saccharomycete

A technology for penicillium and yeast, which is applied in the fields of application, fungi, and fruit and vegetable preservation, etc., to achieve strong antagonism, avoid harm to people and the environment, and be easy to operate

Active Publication Date: 2017-12-29
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Septoria pseudopinnata 16 has been preserved in the General Microorganism Center of China Committee for Culture Collection of Microorganisms (CGMCC)

Method used

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  • Saccharomycete for controlling postharvest penicilliosis of malus domestica and application of saccharomycete
  • Saccharomycete for controlling postharvest penicilliosis of malus domestica and application of saccharomycete
  • Saccharomycete for controlling postharvest penicilliosis of malus domestica and application of saccharomycete

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1: the microbiological characteristics of Septoria pseudopina:

[0021] 1. Morphological features

[0022] (1) Cultivate on NYDA solid medium plate (yeast extract 5g, beef extract 8g, glucose 10g, distilled water 1000mL, agar 20g, 121°C damp heat sterilization for 20min) at 28°C for 48h, the colonies are round and pink The colonies are relatively moist and easy to stir up. The cells are oblong in shape and scattered individually.

[0023] (2) After culturing in NYDB liquid medium for 24 hours, no mold was formed, and the bacterial solution was turbid with precipitation. Microscopically, the yeast cells were oval and budded.

[0024] 2. Molecular biological identification

[0025] Sequence analysis of the 5.8S rDNA-ITS region of the screened strain S. pararoseus 16 was searched on GenBank, and it was identified as Sporidiobolus pararoseus. According to the retrieved homologous strains, the Mege5.1 program of DNAStar software was used to construct the biolo...

Embodiment 2

[0027] Embodiment 2: the safety study of Septoria pseudopinnata 16

[0028] The animals used are ICR mice, provided by the Experimental Animal Center of Jiangsu University, clean grade. Each experimental group consisted of 20 mice, half male and half male. Adopt the way of gavage, according to 0.1mL / l0g body weight (10000mg / kg dosage group presses 0.2mL / 10g body weight) to give test substance, the concentration of yeast is made into 1×10 9 cells / mL of bacterial suspension, and the control group (CK) was filled with the same amount of sterile water. The experiment continued for 15 days, and the mice infused with yeast did not die within 15 days, and the mice did not appear to be poisoned during the whole experiment. According to the national standard of acute toxicity test, it can be judged that the experimental yeast is safe and non-toxic. The experimental results are shown in Table 1.

[0029] Table 1 Toxicity test on mice of S. pseudopinnata 16

[0030]

Embodiment 3

[0031] Embodiment 3: the control effect of Sclerosporium pseudopina to postharvest blue mold of apples

[0032] The activated yeast was inoculated into a conical flask filled with 50 mL of NYDB (5 g of yeast extract, 8 g of beef extract, 10 g of glucose, and 1000 mL of distilled water), and cultured on a shaker at 28°C and 180 rpm for 20 h. Take the bacterial solution and centrifuge it at 8000rpm for 10min, discard the supernatant, wash it repeatedly with sterile water for 3 times, and count it on the hemocytometer to make a concentration of 1×10 6 / mL to 1×10 9 Bacterial suspension per mL, three wounds (5mm×3mm) with uniform distribution, uniform size and depth were made on the equatorial part of the apple fruit surface with a sterilized puncher. Use a pipette to inject 30 μL (1) S. pararoseus yeast suspension (concentration 1×10 6 -10 9 Individuals / mL four gradients); (2) sterile distilled water (control). After standing for 2 hours, inject 30 μL of P.expansum spore susp...

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Abstract

The invention discloses saccharomycete for controlling postharvest penicilliosis of malus domestica and an application of the saccharomycete, and belongs to the technical field of biological control. The saccharomycete is identified as Sporidiobolus pararoseus with the preservation number being CGMCC No. 2.5351. During use, the Sphaerotheca macrophylla is activated, cultured by an NYDB medium for 20 hours and then centrifuged, a thallus is obtained and diluted with sterile water, and the saccharomycete is obtained. With adoption of a saccharomycete suspension and extended penicillium spores for treating the malus domestica, the malus domestica is stored at 20 DEG C for 6 days, and results show that the higher the saccharomycete concentration is, the lower the decay rate is; when the saccharomycete concentration is 1*10<9> cells / mL, the penicilliosis decay rate of the malus domestica is 10.3%, and the penicilliosis decay rate of the control is 100%. Therefore, the Sporidiobolus pararoseus 16 can replace chemical fungicides to control occurrence of the postharvest penicilliosis of the malus domestica, harm of the chemical fungicides to people is avoided, and remarkable economic and social benefits are achieved.

Description

technical field [0001] The invention relates to Sporidiobolus pararoseus 16, which can control the occurrence of postharvest blue mold of apples and belongs to the technical field of biological control. Background technique [0002] Apple (Malus domestica) is a plant of the genus Malus in the family Rosaceae. The fruit is rich in nutrients, including trace elements such as zinc and iron. It has the reputation of "the king of fruits" and is deeply loved by people from all over the world. At present, my country is the largest apple-growing country in the world. However, the areas where apples are grown in my country are relatively scattered. Therefore, in the process of apple purchase and transportation, due to its poor storage capacity and lack of specialized refrigerated transportation tools, it has a great impact on the apple industry. The damage caused was enormous. According to incomplete statistics, the annual rot loss of fruit and vegetable storage in developed countrie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16A23B7/155C12R1/645
CPCA23B7/155A23V2002/00C12N1/145C12R2001/645A23V2200/10
Inventor 赵利娜张红印张晓云李侨飞孙艺文任晓锋
Owner JIANGSU UNIV
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