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Method for positioning immune tissues of growth hormone for malus plants and application thereof

A plant tissue, apple technology, applied in the field of apple auxin immunotissue localization, can solve the problems of long-term preservation of slices, application limitations and the like

Active Publication Date: 2011-08-10
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, fluorescently labeled secondary antibodies are only suitable for observing the distribution of antigens in tissues at the level of light microscopy. Not only are they easily affected by some autofluorescence in the slices, but the slices cannot be preserved for a long time, which limits its application.

Method used

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  • Method for positioning immune tissues of growth hormone for malus plants and application thereof
  • Method for positioning immune tissues of growth hormone for malus plants and application thereof
  • Method for positioning immune tissues of growth hormone for malus plants and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0047] Embodiment 1, IAA immunohistochemical localization of Malus plants

[0048] 1. Fixed

[0049] The small golden crabapple tissue culture seedlings were cultivated in the growth medium (MS+0.5mg / L IBA+0.2mg / L 6-BA), and after the stems were lignified, they were transferred to the rooting medium (1 / 2MS+1.0 mg / L IBA), after the tissue-cultured seedlings gave birth to white roots, they were transferred to 1 / 2 total nutrient solution (see Table 2 for the composition, and the initial pH value was adjusted to 6.0 with NaOH) for 2 weeks, and then transferred to the total nutrient solution solution (the composition is shown in Table 1, and the initial pH value was adjusted to 6.0 with NaOH) for one month, and the complete nutrient solution was changed once a week. -2 ·S -1 ) at a temperature of 25±2°C; cultivate in the dark for 8 hours at a temperature of 17±2°C. Afterwards, iron deficiency induction was carried out. The method was as follows: the plants were transferred from ...

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Abstract

The invention discloses a method for positioning immune tissues of growth hormone for malus plants and application thereof. The method for positioning immune tissues of growth hormone for malus plants comprises the following steps of: (1) fixing plant tissues to obtain fixed plant tissues; (2) carrying out slice making on the fixed plant tissues on the basis of the step (1) to obtain a plant tissue slice; and (3) immunostaining the obtained plant tissue slice on the basis of the step (2), and determining the distribution condition of hormone in the plant tissues through observing a position of a positive signal. The method for positioning immune tissues of growth hormone for malus plants can eliminate the easily caused influence of nonspecific staining of woody plant tissues, and can identify the positive signal clearly. The method for positioning immune tissues of growth hormone for malus plants verifies that the growth hormone has tissue specificity when being distributed in the tissues of a stem tip or a root tip of an apple tree in a condition of iron deficiency.

Description

technical field [0001] The invention relates to a method for localizing the immune tissue of the auxin of the genus Malus and its application. Background technique [0002] Phytohormones are some trace organic substances synthesized in plants that can be transported from the production site to the action site, and have significant physiological effects on growth and development at low concentrations, which can regulate the entire process of plant life activities. Therefore, the study of plant hormones is not only a way to understand the basic laws of plant growth and development, but also an effective means of plant genetics, chemistry and environmental regulation. Therefore, the research and application of plant hormones has very important theoretical significance and broad application prospects. Nearly a century of research has described their different biological effects in detail, and a large number of analytical chemistry results have also clarified the chemical nature ...

Claims

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Application Information

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IPC IPC(8): G01N33/74G01N33/577G01N33/531G01N1/30
Inventor 韩振海吴婷王忆贾文锁张新忠
Owner CHINA AGRI UNIV
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