The present invention provides genes that
encode the N-acetyl-(R,S)-β-
amino acid acylases. The N-acetyl-(R,S)-β-
amino acid acylases were isolated and purified from bacterial cells and the
nucleotide sequences were determined. A host, such as
Escherichia coli, was used to construct a high-expression
system for these genes. The N-acetyl-(R)-β-
amino acid acylase produced by
Burkholderia sp. AJ110349 (FERM BP-10366) includes, for example, the
protein having the amino acid sequence shown in SEQ. ID. NO. 8. The
gene encoding this
enzyme includes, for example, the
DNA having the
nucleotide sequence as shown in SEQ. ID. NO. 7. The N-acetyl-(S)-β-amino acid acylase produced by
Burkholderia sp. AJ110349 (FERM BP-10366) includes, for example, the
protein having the amino acid sequence shown in SEQ. ID. NO. 10. The
gene encoding this
enzyme includes, for example, the
DNA having the
nucleotide sequence shown inshown in SEQ. ID. NO. 9. The N-acetyl-(R)-β-amino acid acylase produced by Variovorax sp. AJ110348 (FERM BP-10367) includes, for example, the
protein comprised of the amino acid sequence shown in SEQ. ID. NO. 12. The
gene encoding this
enzyme includes, for example, the
DNA having the nucleotide sequence shown inshown in SEQ. ID. NO. 11.