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Application of polypeptide in preparation of preparation for preventing and treating plant diseases

A plant disease and preparation technology, which is applied in the field of preparation of peptides for the prevention and treatment of plant disease preparations, can solve the problems of different functions, differences, no references, etc., achieve broad application prospects, and do not produce the effect of drug resistance

Active Publication Date: 2021-10-22
江苏嘉肽生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Another example is the antimicrobial peptide whose APD ID is AP00546 in the APD antimicrobial peptide database. The sequence length of the above-mentioned polypeptide is also 19, and its antibacterial spectrum range is different from the above. It can be seen that for polypeptides with different sequence lengths, or even the same sequence length, the difference in amino acid composition sequence will lead to differences in function. Different peptides have different characteristics, and their antibacterial spectrum needs to be studied according to their own characteristics, so there is no reference between different peptides
[0010] The applicant found in the research that the polypeptide of the present invention has obvious preventive and therapeutic effects on amyrrhia blight, and the prior art does not disclose that it can be used for the prevention and treatment of plant diseases

Method used

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  • Application of polypeptide in preparation of preparation for preventing and treating plant diseases
  • Application of polypeptide in preparation of preparation for preventing and treating plant diseases
  • Application of polypeptide in preparation of preparation for preventing and treating plant diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The effect measurement of embodiment 1 different concentration polypeptides to Erwinia amylovora

[0041] Select the net concentration of the polypeptide as 1.000g / L, 0.500g / L, 0.100g / L, 0.050g / L, 0.010g / L, 0.005g / L, 0.001g / L.

[0042] Streak the plate of Erwinia amylovora XJSZ0102, pick a single colony on the fresh plate and put it in 3mL LB liquid medium, culture at 27°C, 200rpm for 36h. Adjust the OD600 of the bacterial solution to 0.4±0.01, and then dilute it 10000 times as the spare bacteria. When acting, the final concentration of bacteria was 10 6 CFU / mL.

[0043] Preparation of neutralizing reagents. 10mL system: Aseptically weigh 0.2g lecithin, add 300μL absolute ethanol to dissolve, add 9.5mL LB liquid medium, 200μL Tween-80, shake well. The above actions are all completed in the ultra-clean bench.

[0044] Preparation of the sample group: Take 1mL of polypeptide in a 1.5mL EP tube, add 100μL of spare bacterial solution, shake it well and let it stand for...

Embodiment 2

[0050] Embodiment 2 polypeptide to Erwinia amylovora MIC experiment

[0051] Prepare NB sterile medium containing 64mg / L polypeptide, and use NB medium to dilute to 32mg / L, 16mg / L, 8mg / L, 4mg / L, 2mg / L, and add 400μL of medium to each concentration In a 96-well plate, add 200 μL to each well. Adjust the concentration of activated Erwinia amylovora XJSZ0102 to 1×10 6 cfu / ml to prepare bacterial suspension and add 10 μL to each well. At the same time, a positive control containing bacteria and no polypeptide and a blank control containing no bacteria and no polypeptide were set. Placed in a 28°C, 150rpm constant temperature shaking incubator for 48h.

[0052] see results figure 1 .

[0053] The positive control and the 2mg / L polypeptide group grew bacteria, and the other groups grew sterile.

[0054] According to the results of MIC experiments, it can be concluded that when the effective concentration of the polypeptide reaches 4 mg / L, a higher antibacterial efficiency agai...

Embodiment 3

[0055] Bactericidal effect on embodiment 3 greenhouse Du pear seedlings

[0056] Prepare the bacterial liquid, take the Erwinia amylovora XJSZ0102 stored in the refrigerator at -70°C, streak on the NA plate medium for activation culture, place it in the incubator (28°C, dark) and cultivate it for 48 hours, then pick a single colony , inoculated in NB liquid medium, 28°C, 150rpm shaking culture for 24h.

[0057] For the preparation of the polypeptide concentration, take the polypeptide and prepare it with sterile water to prepare a polypeptide solution with a concentration of 1 g / L for later use.

[0058] Agricultural streptomycin is the main agent for the prevention and control of amylovora blight, and its control effect on amylovora blight is better than other pesticides, so it was compared with the bactericidal effect of peptides on Erwinia amylovora in greenhouse Du pear seedlings. The concentration of agricultural streptomycin in this experiment was 0.25g / L.

[0059] Che...

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Abstract

The invention relates to the technical field of plant disease prevention and control, in particular to application of polypeptide to preparation of a preparation for preventing and treating plant diseases; the preparation is prepared by taking polypeptide as an active component and adding auxiliary materials or auxiliary components. The preparation disclosed by the invention has obvious prevention and treatment effects on plant diseases caused by Erwinia amylovora, and has outstanding prevention and treatment effects on fire blight of rosaceae plants, especially economic crops such as pears, apples, haws and malus spectabilis. Compared with the prior art, the polypeptide preparation has the effects of wide action range, high safety, no biotoxicity, no drug resistance, no stimulation and no hormone, and can effectively prevent and treat plant diseases.

Description

technical field [0001] The invention relates to the technical field of plant disease prevention and control, in particular to the use of a polypeptide in the preparation of preparations for preventing and treating plant diseases. Background technique [0002] Plant disease refers to the phenomenon that plants undergo a series of morphological, physiological and biochemical pathological changes under the influence of biological or abiotic factors, hindering the normal growth and development process, thereby affecting human economic benefits. Among them, the amylovora blight caused by Erwinia amylovora was the first identified plant bacterial disease. Pear fire blight is a destructive disease, and it is a kind of dangerous pest for imported plants in my country. It has always been an important bacterial disease on pears, apples and other fruit trees in North America. [0003] Erwinia amylovora, also known as Phytophthora amylovora, has a wide range of hosts and can infect mor...

Claims

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Application Information

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IPC IPC(8): A01N37/46A01N47/44A01P1/00
CPCA01N37/46A01N47/44
Inventor 凌建群楼兵干
Owner 江苏嘉肽生物技术有限公司
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