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A Seedling Hardening Method for Significantly Improving the Transplanting Survival Rate of Cassava Tissue Cultured Seedlings

A technique for transplanting survival rate and tissue culture seedlings, applied in horticultural methods, botanical equipment and methods, cultivation and other directions, can solve problems such as poor soil adaptability, low survival rate, lodging of stems, etc., and reduce foliar transpiration. , The root system is thick and developed, and the leaves are dark green and lush.

Active Publication Date: 2022-05-03
HAINAN UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In view of this, the present invention provides a seedling hardening method that significantly improves the transplanting survival rate of cassava tissue cultured seedlings, which solves the problems caused by root damage, stalk lodging, poor adaptability of soil, etc. during the transplanting process of existing cassava tissue cultured seedlings. The problem of low survival rate caused by

Method used

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  • A Seedling Hardening Method for Significantly Improving the Transplanting Survival Rate of Cassava Tissue Cultured Seedlings
  • A Seedling Hardening Method for Significantly Improving the Transplanting Survival Rate of Cassava Tissue Cultured Seedlings
  • A Seedling Hardening Method for Significantly Improving the Transplanting Survival Rate of Cassava Tissue Cultured Seedlings

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Experimental program
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Effect test

Embodiment 1

[0025] Embodiment one: a kind of hardening method that significantly improves the transplanting survival rate of cassava tissue-cultured seedlings comprises the following steps: (1) selection and breeding: the cassava tissue-cultured seedlings grown for 50 to 60 days are cut into 1.5 to 2.5 cm test tubes Seedling stems, and keep the leaves on the stems, subcultured in seedling culture medium (MS basic medium + 0.1mg / L paclobutrazol pp333) for 33 to 37 days to obtain cassava tissue culture seedlings suitable for transplanting. The average seedling height of the tissue cultured seedlings was 5.24cm, and the growth was good, the leaves were dark green, the stems were thick and thick, the roots were short and strong, the average root system length was 2.7cm, and the average root length was 5.45.

[0026] (2) Colonization: take (1) cassava tissue culture seedlings out of the tissue culture bottle, wash the medium, and be careful not to damage the root system. Then with a diameter o...

Embodiment 2

[0029] Embodiment two: the difference with embodiment one is that the concentration of paclobutrazol in the breeding step (1) is respectively MS, MS+0.05mg / L, MS+0.15mg / L, MS+0.2mg / L, MS+0.25mg / L, MS+0.3mg / L, the results are shown in Table 1, within the concentration range of 0-0.25mg / L, with the increase of PP333 concentration, the leaves become thicker, the leaf color deepens, the plant height shortens significantly, and the root system becomes thicker , the number of roots increased significantly; when the concentration of PP333 was in the range of 0.25mg / L to 0.35mg / L, the leaves were dark green and thick, but some leaves would curl and shrink. It shows that with the increase of the concentration of PP333, the tissue culture seedlings will gradually grow stronger, but the concentration is too high, but it will affect the health of the tissue culture seedlings. It can be obtained from the table that adding PP333 at a concentration of 0.1 mg / L can most effectively improve t...

Embodiment 3

[0033]Embodiment three: the difference with embodiment one is that the thickness of the cotton planted in step (2) is respectively 3cm, 2cm, 1.5cm, 0.75cm and 0cm, as shown in Table 2 by experimental result, the cotton planted too thick (more than 1.5cm ), the contact parts between the young stems of cassava seedlings and the planted cotton will rot, causing the leaves to wilt, lodging and eventually die; and the planted cotton is too thin (below 0.75cm), it cannot play a role in fixing, the tissue culture seedlings are easy to lodging, the leaves wilt, the stems The stalk is bent, the root is not strong, the slow seedling stage is delayed, and even dies; while the planted cotton is 1cm, the fixation effect is good, the leaves stand upright, the root grows stably, the slow seedling stage is greatly shortened, and it has strong adaptability and resistance to the environment. inverse.

[0034] Table 2 Effects of different planting cotton thicknesses on cassava tissue culture see...

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Abstract

The invention discloses a seedling hardening method for significantly improving the transplanting survival rate of cassava tissue cultured seedlings, which comprises the following steps: retaining the mature stem segments of leaves of cassava, subcultured on a seedling culture medium for 33 to 37 days to obtain tissue cultured seedlings; Take out the tissue-cultured seedlings and wash them, clamp the stems with planting cotton at a place 0.5cm higher than the root system, and transplant them into peat soil pits for planting; cover the potted plants with fresh-keeping bags, shade them all the way, and keep moisture After a few days, the bag is removed, and the planting process and 6-12 hours after planting are carried out indoors; after removing the bag, cultivate for 12-16 days, transfer the cassava plants to the formula soil and remove the planted cotton, and cultivate for 14-21 days to complete refining. Seedlings, the formula soil is 4:1:1:1:1 peat soil, humus soil, vermiculite, perlite and red soil. Through the controllable subculture of the mature stems of the cassava leaves, and then using the cotton planted in the peat soil, and then transferred to a specific formula soil to cultivate good quality seedlings, the survival rate was significantly increased from 50% to 95%. %.

Description

technical field [0001] The invention relates to the fields of agricultural technology and tissue culture, in particular to a seedling hardening method capable of significantly improving the transplanted survival rate of cassava tissue cultured seedlings. Background technique [0002] Cassava is a plant of the genus Cassava in the Euphorbiaceae family. It is drought-resistant and barren-resistant. It is an important starch crop and is widely planted in many countries and regions such as Africa, America, and Asia. It is one of the three major potato crops in the world. Granary" and "King of Starches". Cassava has a wide range of uses, not only as food such as cassava rice, but also as important industrial raw materials such as cassava root starch, dry flakes that can be modified starch, alcohol and sorbitol, etc., and these products are all used in food, medicine, textile and paper industries. important raw material. However, the average yield of cassava is relatively low. I...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G31/00A01G22/25A01G24/28A01G24/15A01G24/10
CPCA01H4/008A01H4/001A01G31/00A01G22/25A01G24/28A01G24/15A01G24/10
Inventor 闵义曹鹏刘肖寒姬卿胡新文黄武李双宝
Owner HAINAN UNIVERSITY
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