Method for establishing general maize regeneration system
A corn and system technology, applied in plant regeneration, horticultural methods, botanical equipment and methods, etc., can solve the problems of difficult direct utilization, poor comprehensive economic properties, etc., and achieve the effect of improving the effect, increasing the survival rate, and reducing the cost
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Embodiment 1
[0030] Example 1: Establishment of general corn regeneration system
[0031] (1) Surface disinfection: select Condy 3 and Condy 4 of excellent corn inbred line seeds. The young ears can be used for 8-10 days after pollination. At this time, the length of the immature embryo of the immature seeds is 1.0-2.0mm. The outer bracts of the ear are removed, and the young ears are surface-disinfected with 70% alcohol, and rinsed with sterile water for 1-2 times .
[0032] (2) Induction of corn callus and embryogenic callus: Use dissecting tweezers to carefully pick out immature embryos on an ultra-clean workbench, connect the sterilized immature embryos to the induction medium, and incubate at 28°C for 2-3 Week; Induction medium formula adopts: N6 macroelement, B5 trace element, B5 vitamin, 0.04mg / L riboflavin, 0.04mg / L folic acid, 0.1mg / L niacin, 0.1mg / L D-calcium pantothenate, 0.04 mg / L p-toluic acid, sucrose 50g / L, 2.4-D is 0.4mg / L, 6-BA is 5mg / L. Condy 3 and Condy 4 induced white cal...
Embodiment 2
[0036] Example 2: Zheng 958, Establishment of Xianyu 335 corn regeneration system
[0037] (1) Surface disinfection: select Zheng 958 and Xianyu 335, which are excellent corn inbred line seeds. The young ears can be used for 9-11 days after pollination. At this time, the length of the immature embryo of the immature seeds is 1.0-2.0mm. The outer bracts of the ear are removed, and the young ears are surface-disinfected with 70% alcohol and rinsed with sterile water for 1-2 times .
[0038] (2) Induction of corn callus and embryogenic callus: Use dissecting tweezers to carefully pick out immature embryos on an ultra-clean workbench, connect the sterilized immature embryos to the induction medium, and incubate at 28°C for 2-3 Week; Induction medium formula adopts: N6 macroelement, B5 trace element, B5 vitamin, 0.04mg / L riboflavin, 0.04mg / L folic acid, 0.1mg / L niacin, 0.1mg / L D-calcium pantothenate, 0.04 mg / L p-toluic acid, sucrose 50g / L, 2.4-D is 0.4mg / L, 6-BA is 5mg / L. Zheng 958 a...
Embodiment 3
[0042] Example 3: Establishment of A188 and Syn 31 corn regeneration system
[0043] (1) Surface disinfection: Select the seeds of excellent maize inbred lines A188 and Zong 31. The young ears can be used for 8-11 days after pollination. At this time, the length of the immature embryo of the immature seeds is 1.0-2.0mm. The outer bracts of the ear are removed, and the young ears are surface-disinfected with 70% alcohol and rinsed with sterile water for 1-2 times .
[0044] (2) Induction of corn callus and embryogenic callus: Use dissecting tweezers to carefully pick out immature embryos on an ultra-clean workbench, connect the sterilized immature embryos to the induction medium, and incubate at 28°C for 2-3 Week; Induction medium formula adopts: N6 macroelement, B5 trace element, B5 vitamin, 0.04mg / L riboflavin, 0.04mg / L folic acid, 0.1mg / L niacin, 0.1mg / L D-calcium pantothenate, 0.04 mg / L p-toluic acid, sucrose 50g / L, 2.4-D is 0.4mg / L, 6-BA is 5mg / L. White callus was induced in...
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