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Method for establishing general maize regeneration system

A corn and system technology, applied in the directions of plant regeneration, horticultural methods, botanical equipment and methods, etc., can solve problems such as difficulty in direct utilization, poor comprehensive economic properties, etc., and achieve improved effect, increased survival rate, and reduced organic components. Effect

Active Publication Date: 2012-12-19
新疆康地种业科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In summary, although maize regeneration systems are relatively mature, almost all reported maize regeneration systems are limited to a limited number of maize inbred lines, and their comprehensive economic traits are poor, so it is difficult to directly use them in breeding.

Method used

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  • Method for establishing general maize regeneration system
  • Method for establishing general maize regeneration system
  • Method for establishing general maize regeneration system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment one: the establishment of general corn regeneration system

[0031] (1) Surface disinfection: Condy3 and Condy4 are selected from the excellent inbred lines of maize. The young ears can be 8-10 days after pollination. At this time, the length of the young embryos of the immature seeds is 1.0-2.0 mm. The outer bracts of the ears are stripped off, and the young ears are surface-sterilized with 70% alcohol, and rinsed with sterile water for 1-2 times. .

[0032] (2) Induction of corn callus and embryogenic callus: Carefully pick out immature embryos with dissecting tweezers on an ultra-clean workbench, connect the sterilized immature embryos to the induction medium, and culture them for 2-3 days at 28°C. Zhou; Induction medium formula used: N6 macroelement, B5 trace element, B5 vitamin, 0.04mg / L riboflavin, 0.04mg / L folic acid, 0.1mg / L niacin, 0.1mg / L D-calcium pantothenate, 0.04 mg / L p-toluic acid, sucrose 50g / L, 2.4-D 0.4mg / L, 6-BA 5mg / L. Condy3 and Condy 4...

Embodiment 2

[0036] Example 2: Establishment of Zheng 958 and Xianyu 335 corn regeneration systems

[0037] (1) Surface disinfection: Zheng 958 and Xianyu 335 were selected as seeds of excellent maize inbred lines. 9-11 days after pollination, the young ears can be used. At this time, the length of the young embryos of immature seeds is 1.0-2.0mm. The outer bracts of the ears are peeled off, and the young ears are surface-sterilized with 70% alcohol, and rinsed with sterile water for 1-2 times. .

[0038] (2) Induction of corn callus and embryogenic callus: Carefully pick out immature embryos with dissecting tweezers on an ultra-clean workbench, connect the sterilized immature embryos to the induction medium, and culture them for 2-3 days at 28°C. Zhou; Induction medium formula used: N6 macroelement, B5 trace element, B5 vitamin, 0.04mg / L riboflavin, 0.04mg / L folic acid, 0.1mg / L niacin, 0.1mg / L D-calcium pantothenate, 0.04 mg / L p-toluic acid, sucrose 50g / L, 2.4-D 0.4mg / L, 6-BA 5mg / L. Zh...

Embodiment 3

[0042] Embodiment three: the establishment of A188, comprehensive 31 corn regeneration system

[0043] (1) Surface disinfection: the seeds of excellent maize inbred lines A188 and Zong 31 were selected. 8-11 days after pollination, the young ears can be used. At this time, the length of the young embryos of the immature seeds is 1.0-2.0mm. The outer bracts of the ears are peeled off, and the young ears are surface-sterilized with 70% alcohol, and rinsed with sterile water for 1-2 times. .

[0044] (2) Induction of corn callus and embryogenic callus: Carefully pick out immature embryos with dissecting tweezers on an ultra-clean workbench, connect the sterilized immature embryos to the induction medium, and culture them for 2-3 days at 28°C. Zhou; Induction medium formula used: N6 macroelement, B5 trace element, B5 vitamin, 0.04mg / L riboflavin, 0.04mg / L folic acid, 0.1mg / L niacin, 0.1mg / L D-calcium pantothenate, 0.04 mg / L p-toluic acid, sucrose 50g / L, 2.4-D 0.4mg / L, 6-BA 5mg / L...

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Abstract

The invention discloses a method for establishing a general maize regeneration system. The method comprises the following steps of: inoculating different maize inbred line embryos onto induction mediums with different hormone combinations, transferring an appropriate induced embryoid onto a differential medium to carry out high frequency differentiation on regenerated seedlings, transferring the regenerated seedlings onto a rooting medium to carrying out rooting induction so as to obtain a regenerated plant, wherein the formula of the induction medium comprises N6 marcoelement, B5 microelement, B5 vitamin, riboflavin (0.04mg / L), folic acid (0.04mg / L), nicotinic acid (0.1mg / L), D-calcium pantothenate (0.1mg / L), methyl benzoic acid (0.04mg / L), cane sugar (50g / L), 2.4-D (0.4mg / L) and 6-BA (5mg / L). The method disclosed by the invention has the characteristics of good generality to different maize inbred lines and shorter time for obtaining the regenerated plant and has important application value.

Description

field of invention [0001] The invention relates to the field of biotechnology, in particular, the invention specifically relates to the technical field of establishing a universal corn regeneration system. Background technique [0002] Corn is the third largest food crop in the world, second only to rice and wheat in planting area. At the same time, corn is an important feed crop. Therefore, increasing corn production and improving corn quality is related to China's future food security supply. Genetic improvement of maize through conventional breeding is one of the main ways to increase maize production, but factors such as limited genetic resources and relatively long breeding cycles often limit its application potential. With the rapid development of biotechnology, breeders from various countries have widely used biotechnology to introduce some exogenous genes that control excellent traits into maize, so as to achieve the purpose of cultivating new maize varieties with ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 王沛政林霞夏春兰王志安李新鹏
Owner 新疆康地种业科技股份有限公司
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