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Method capable of promoting Trichosanthes kirilowii tissue culture seedling differentiation

A technology of melon and culture medium is applied in the field of promoting the differentiation of cultivated seedlings of melon, which can solve the problems of long time for seed propagation, uncoordinated ratio of male and female plants, slow root propagation and germination, etc., so as to accelerate wound healing, increase The effect of using many ways and results

Inactive Publication Date: 2017-07-21
蚌埠清菲农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the planting method of Trichosanthes mostly adopts seed propagation and split-root propagation, which takes a long time for seed propagation, and the ratio of male and female plants is seriously uncoordinated, and the germination of split-root propagation is slow, and the tubers need to be dug out, wasting a lot of trichosanthes pollen At present, the tissue culture rapid propagation technology of Trichosanthes trichosanthes has just started, and it takes 25 to 30 days to induce cell protrusions in the stage of induction and differentiation, which takes a long time. Therefore, a method for promoting the differentiation of Trichosanthes trichosanthes group culture seedlings is needed

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A method for promoting the differentiation of Trichosanthes group cultured seedlings, comprising the following steps:

[0021] (1) Selection and treatment of explants: Select excellent plants with vigorous growth, more fruits, and strong stress resistance, and cut young leaves from the petiole, which will not cause damage to the Trichosanthes plant, and will not affect the growth and development of Trichosanthes. As a result, tender leaves come from a wide variety of sources, and tissue culture can be carried out at any stage, and the planting efficiency is significantly improved. They are directly placed in 4°C and 2.1% saline solution for 25 minutes, and then placed at 22°C after taking them out. Soak in 3% acetic acid solution for 40 minutes, rinse with running water for 20 minutes, soak in nutrient solution for 2 hours, and then rinse with sterile water for 3 to 4 times. The nutrition is balanced, the absorption is promoted, and the reproduction of pathogenic microor...

Embodiment 2

[0033] A method for promoting the differentiation of Trichosanthes group cultured seedlings, comprising the following steps:

[0034] (1) Selection and treatment of explants: Select excellent plants with vigorous growth, more fruits, and strong stress resistance, and cut young leaves from the petiole, which will not cause damage to the Trichosanthes plant, and will not affect the growth and development of Trichosanthes. As a result, tender leaves come from a wide range of sources, and tissue culture can be carried out at any stage, and the planting efficiency is significantly improved. They are directly placed in 5°C and 2.2% saline solution for 28 minutes, and then placed at 23°C after taking them out. Soak in 3% acetic acid solution for 40 minutes, rinse with running water for 20 minutes, soak in nutrient solution for 2.5 hours, and then rinse with sterile water for 3 to 4 times. The nutrition is balanced, promotes absorption, inhibits the reproduction of pathogenic microorga...

Embodiment 3

[0046] A method for promoting the differentiation of Trichosanthes group cultured seedlings, comprising the following steps:

[0047] (1) Selection and treatment of explants: Select excellent plants with vigorous growth, more fruits, and strong stress resistance, and cut young leaves from the petiole, which will not cause damage to the Trichosanthes plant, and will not affect the growth and development of Trichosanthes. As a result, the source of young leaves is wide, and tissue culture can be carried out at any period, and the planting efficiency is significantly improved. They are directly placed in 6°C and 2.3% saline solution for 30 minutes, and then placed at 24°C after taking them out. Soak in 4% acetic acid solution for 45 minutes, rinse with running water for 20 minutes, soak in nutrient solution for 3 hours, and then rinse with sterile water for 3 to 4 times. The nutrition is balanced, promotes absorption, inhibits the reproduction of pathogenic microorganisms, and can...

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Abstract

The invention mainly relates to the field of planting technology, and discloses a method for promoting the differentiation of Trichosanthes mellifera seedlings, including: selection and treatment of explants, preparation of medium, inoculation of explants, induction of differentiation and cultivation; Induced differentiation during basket tissue culture, after 16 days of culture in B5 medium, yellow-green cell protrusions began to appear, and then differentiated bud clusters appeared, which significantly shortened the time for induction of differentiation, improved planting efficiency, and increased economic income by 16.2%; As an explant, it will not cause damage to the Trichosanthes plant, does not affect the growth and results of Trichosanthes, and the source of young leaves is extensive, and tissue culture can be carried out at any time, and the planting efficiency is significantly improved; Salt water, acetic acid solution and nutrient solution treatment, avoid using too much disinfectant for repeated disinfection, save costs, and allow explants to continue absorbing and accumulating nutrients in a slightly acidic environment, speeding up the process of inducing differentiation of explants.

Description

technical field [0001] The invention mainly relates to the field of planting technology, in particular to a method for promoting the differentiation of Trichosanthes mellifera group cultured seedlings. Background technique [0002] Gualou, also known as Trichosanthes L., Yao Gua, etc., is a perennial herbaceous teneben whose whole plant is used for medicinal purposes. It is a plant of the genus Trichosanthes family Cucurbitaceae. Whole Gualou is used as medicine to clear away heat and dissipate stagnation, nourish the lungs and resolve phlegm, nourish the stomach and promote body fluids, etc. Trichosanthes injection is clinically used for women's second-trimester pregnancy, stillbirth, and miscarriage, and has a significant inhibitory effect on the growth of cancer cells. [0003] At present, the planting method of Trichosanthes mostly adopts seed propagation and split-root propagation, which takes a long time for seed propagation, and the ratio of male and female plants is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001A01H4/008
Inventor 翟明安李香华
Owner 蚌埠清菲农业科技有限公司
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