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Method for directly inducing adventitious buds of butterfly orchid

A phalaenopsis, direct technology, applied in the field of direct induction of phalaenopsis adventitious buds, can solve the problems of 24-48 hours of continuous operation, increased pollution by antioxidants, complicated procedures, etc., achieves remarkable induction effect, promotes rapid growth, and is easy to operate Effect

Active Publication Date: 2014-08-20
CHINA THREE GORGES CORPORATION +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On January 20, 2012, Tang Dehua, Department of Environment and Life Sciences, Sichuan University for Nationalities, published in "Anhui Agricultural Science" on "Research on Browning Control of Phalaenopsis Explants", on the method of preventing explants from browning Adding adsorbents, anti-browning agents, natural organic matter, adjusting light and culture temperature, and heat treatment of explants, etc., but both have certain shortcomings: activated carbon can effectively control browning, but it is not conducive to differentiation; The addition of oxidants will increase pollution; heat treatment generally requires a design temperature range and time period, and the recovery period requires 24-48 hours of continuous operation, and the procedure is more complicated

Method used

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  • Method for directly inducing adventitious buds of butterfly orchid
  • Method for directly inducing adventitious buds of butterfly orchid
  • Method for directly inducing adventitious buds of butterfly orchid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Adventitious bud induction technology treatment:

[0022] In order to smoothly and effectively promote the formation of adventitious buds, spray 6.44×10 Phalaenopsis plants with reproductive ability (transition from vegetative growth to reproductive reproduction stage) or perennial robust Phalaenopsis plants in the current year. 6 g MS mineral element + hormone combination combination (10g0.01mg / L TDZ+120g0.1mg / L GA3+150g0.1mg / L NAA), MS mineral element spray, hormone spray combination, control 4 treatments, each The treatment included 10 pots of individual plants, and the experiment was repeated 3 times. The treatment time starts from the beginning of October, every 40 days, a total of 3 times of spraying. Use a 150ml small watering can for each spraying, adjust the mouth of the pot to the best state of mist, and aim at the front of the plant leaves to spray evenly from the inside to the outside. After spraying, it is better for the water mist to adhere to the surface...

Embodiment 2

[0031] Adventitious bud induction technology treatment:

[0032] In order to promote the formation of adventitious buds smoothly and effectively, spray 5.2×10 Phalaenopsis plants with reproductive ability (transition from vegetative growth to reproductive reproduction stage) or perennial robust Phalaenopsis plants in the current year. 6 g MS mineral element + hormone combination combination (10g0.05mg / L TDZ+110g0.3mg / L GA3+140g0.4mg / L NAA), MS mineral element spray, hormone spray combination, control 4 treatments, each The treatment included 10 pots of individual plants, and the experiment was repeated 3 times. The treatment time starts from the beginning of October, every 40 days, a total of 3 times of spraying. Use a 150ml small watering can for each spraying, adjust the mouth of the pot to the best state of mist, and aim at the front of the plant leaves to spray evenly from the inside to the outside. After spraying, it is better for the water mist to adhere to the surface ...

Embodiment 3

[0040]Adventitious bud induction technology treatment:

[0041] In order to promote the formation of adventitious buds smoothly and effectively, spray 5.25×10 Phalaenopsis plants with reproductive ability (transition from vegetative growth to reproductive reproduction stage) or perennial robust Phalaenopsis plants in the current year. 6 g MS mineral element + hormone combination combination (10g0.05mg / L TDZ+100g0.5mg / L GA3+100g0.45mg / L NAA), MS mineral element spray, hormone spray combination, control 4 treatments, each The treatment included 10 pots of individual plants, and the experiment was repeated 3 times. The treatment time starts from the beginning of October, every 40 days, a total of 3 times of spraying. Use a 150ml small watering can for each spraying, adjust the mouth of the pot to the best state of mist, and aim at the front of the plant leaves to spray evenly from the inside to the outside. After spraying, it is better for the water mist to adhere to the surface...

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Abstract

After butterfly orchid is treated by an outdoor hormone combination (TDZ+GA3+NAA) and a browning prevention solution (AC+PVP+Vc0.5g / L+glutathione), adventitious buds can be directly induced, the content of explant total phenols and PAL activity can be inhibited, and rapid plant growth is promoted. Due to the outdoor hormone treatment, hormones can be absorbed by the explant in advance, hormonal equilibrium needed by adventitious bud germination is achieved, and the time needed for absorbing nutrition in a bottle is shortened. Browning generated in the explant is greatly inhibited due to the outdoor treatment, so that rapid growth of the adventitious buds and seedlings is promoted. According to the explant, the adventitious buds of the butterfly orchid are directly induced without passing a callus stage, excellent traits of stock plants can be kept, variation generated in the culture process is reduced, and the method has high significance in large-scale production.

Description

technical field [0001] The invention relates to a method for directly inducing adventitious buds of Phalaenopsis, belonging to the technical field of biological induction. Background technique [0002] Phalaenopsis spp. is a flower of the genus Phalaenopsis in the family Orchidaceae. Because of its unique flower shape, bright colors and long-lasting flowering period, it is known as the "Queen of Yanglan". [0003] According to Wang Changyun, Yantai Academy of Agricultural Sciences, Shandong Province on August 15, 2013, in the "Research on Phalaenopsis Tissue Culture and Rapid Propagation Technology System" published in "Northern Horticulture", using seeds, pedicel axillary buds, stem tips, leaves, and pedicel nodes Tissues and organs such as interstitium and root tip are used as explants, which are induced to form protocorms under certain conditions, and then proliferate through protocorms. On January 20, 2012, Tang Dehua, Department of Environment and Life Sciences, Sichu...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 张海波张国禹黄桂云吴笛赵华萍马晓波胡梅香杨兰芳汪磊邱利文李翩翩徐芳
Owner CHINA THREE GORGES CORPORATION
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