86 results about "Virus elimination" patented technology

Fusion of the viral envelope, or infected cell membranes with uninfected cell membranes, is an essential step in the viral life cycle. Recent studies involving the human immunodeficiency virus type 1(HIV-1) demonstrated that synthetic peptides (designated DP-107 and DP-178) derived from potential helical regions of the transmembrane (TM) protein, gp41, were potent inhibitors of viral fusion and infection. A computerized antiviral searching technology (C.A.S.T.) that detects related structural motifs (e.g., ALLMOTI 5, 107x178x4, and PLZIP) in other viral proteins was employed to identify similar regions in the Epstein-Barr virus (EBV). Several conserved heptad repeat domains that are predicted to form coiled-coil structures with antiviral activity were identified in the EBV genome. Synthetic peptides of 16 to 39 amino acids derived from these regions were prepared and their antiviral activities assessed in a suitable in vitro screening assay. These peptides proved to be potent inhibitors of EBV fusion. Based upon their structural and functional equivalence to the known HIV-1 inhibitors DP-107 and DP-178, these peptides should provide a novel approach to the development of targeted therapies for the treatment of EBV infections.

The invention relates to the molecular medicine and gene treating field, in particularly, a gene segment which is new, cell target special, and can eliminate replication ability and synthesizing ability of nucleocapsid protein from source and clean the pollution protein. The gene segment is composed of a recombinant aden-associated virus vector helper plasmid non-structure and/or structure gene, and at least one copy cell special microRNA target sequence which can be used in multiple cell strains directly, and also can be used for constructing recombinant aden-associated virus plasmid and vector to prepare medicine, basic medicine and clinic gene treating.

The invention discloses a network information security protection system based on the Internet. The network information security protection system comprises a cloud platform, a registration and loginunit, a database, an information auditing unit, an intrusion detection module, a user management module, an alarm unit and a protection unit. The method comprises the following steps: detecting systemdata through an intrusion detection module, acquiring illegal access times, virus type quantity and vulnerability quantity of a system, acquiring a system intrusion coefficient Y through a formula, and comparing the system intrusion coefficient Y with an intrusion coefficient threshold value: if the system intrusion coefficient Y is less than or equal to the intrusion coefficient threshold value,judging that intrusion does not exist in the system; generating a system security signal and sending the system security signal to the cloud platform; if the system intrusion coefficient Y is greaterthan the intrusion coefficient threshold, judging that intrusion exists in the system, generating a system danger signal and sending the system danger signal to an alarm unit. After the system is detected, viruses are eliminated before data loss of the system, so that the data security is improved, and the risk of data loss is reduced.

The invention belongs to the technical field of computer defense, in particular relates to a method and a system for preventing computer virus from frequently infecting systems. The method comprises the following steps of: scanning and deleting the spite model of the computer virus in a computer, and then recording the information of the deleted spite model; generating an interception object list according to the information of the deleted spite model and transmitting the interception object list to a kernel driver; and adopting the kernel driver to prevent other programs from secondly building the spite model which is recorded in the deleted model. The model corresponding to the system of the invention comprises a virus scanning, deleting and recording model, an interception list generation model and a generation interception model of the spite model. The invention can effectively delete the spite virus which has generation capability; at the same time, for enterprise-level servers, the invention can effectively delete the damage of the virus under the condition that the system is not restarted so as to ensure the safety of data and service.

The invention discloses a method for strawberry virus-removing, comprising the following steps: 1) putting the potted strawberry with virus into the bio-chemical incubator, culturing in the lethal temperature for virus for 2-3 days; 2) stripping the stem tip nascent tissue of smaller than 0.5mm, inoculating which in the culture medium with concentration of MS half-reduced for its growing; 3) transferring the stem tip nascent tissue when its sprout grows to 0.5-1.5cm in height to induced enrichment culture medium for growing; transferring it to biochemical incubator when generates adventitious bud, culturing under the lethal temperature for 30 minutes; 4) putting the sprout mentioned above into tissue culture room for culturing under 28 Deg C for 30 minutes; 5) repeating the steps 2) to 4) for 1-2 times. The method in this invention does not strip large quantity of stem tip and the virus-removing rate is as high as 100%.

The invention relates to a method for inducing strawberry stolons and eliminating viruses in a test tube by high temperature processing in combination with shoot tip culture. In the method, the stolons of test-tube plantlets of an excellent new variable variety generated by callus differentiation of deep-mountain strawberry petals are induced in the test tube and the virus elimination of the variety is studied by combining high temperature and shoot tips in the test tube to obtain virus-free strawberry seedlings for production. The method comprises the following steps: inducing the stolons of the obtained new variety in a culture medium in the test tube, wherein the induction rate is above 99.5 percent; culturing the test-tube plantlets containing the stolons for 85d in the test tube at the temperature of 56 DEG C, and cutting 1mm of shoot tip to induce shoot tip calluses in the culture medium; then transferring the shoot tip calluses to the culture medium to perform re-differentiation culture of callus seedlings; and detecting the viruses of the regenerated seedlings, wherein the virus elimination rate is 100 percent. A test result shows that the virus elimination method by high-temperature processing in combination with the shoot tips in the test tube can eliminate the viruses completely so as to establish a strawberry virus elimination system.

The invention discloses a method for preparing human thrombin from cold-removing glue plasma. Firstly, anion resins are used to absorb prothrombin in the cold-removing glue plasma, then elution and filtering are conducted, and a prothrombin solution is obtained; secondly, the prothrombin solution is activated through a CaCL2 solution, and a thrombin solution is obtained; thirdly, S/D viral inactivation is conducted on the thrombin solution; fourthly, chromatography is conducted through cation columns, and a purified thrombin solution is obtained; fifthly, ultrafiltration, dialysis and concentrate are conducted on the thrombin solution, and the thrombin solution with the titer meeting the product specification requirement is obtained; sixthly, a filter element of 20 nm in size is used to conduct virus removal filtering; seventhly, sterilization filtering is conducted through a filter element of 0.22 microns in size, and then subpackage is conducted according to the required specifications; eighthly, freeze-drying is conducted, and dry heat viral inactivation is conducted on freeze-drying powder, and a human thrombin product is obtained. According to the method, the thrombin is purified through the one-step cation columns, operation is simple, thrombin specific activity is high, and safety of clinic use of the product is guaranteed through a three-step virus elimination mode.

Healthy tissue culture seedlings of a scion variety (strain) are cut into bladeless stem segments of 2-3cm at length to be as scions, healthy tissue culture seedlings of a rootstock variety (strain) are cut into bladed stem segments of 2-3cm at length to be as rootstocks, and the stem segments are soaked and pretreated for 10m in a GA3 solution of 0.5-1.0mg/L in concentration; under an aseptic condition, split grafting is used for carrying out micro-grafting and tinfoil is used for binding grafting interfaces; micro-grafted seedlings are vertically inserted into the culture medium to culture, cotton plugs are used for sealing the bottle mouths of culture bottles and then monolayer plastic sealing membrane is covered and tightly bound; the basic culture medium of the micro-grafted seedlings is MS, wherein, the concentration of the sucrose is 50g/L, the pH value before sterilizing of the culture medium is 6.0 and the temperature of a culture chamber is 28 DEG C. The adopted micro-grafting method can improve the survival rate of the grafting to 83 percent, realize scaled rapid filtration of disease-resistant varieties and idioplasm and is beneficial to develop researches such as the relevant rapid testing of pathogen and virus elimination.

The invention provides a method and system for recording transmission paths and distribution conditions of files in a local area network. According to the method and system, host file monitoring is combined with network-level file transmission monitoring, and monitoring data are aggregated to a server for correlation analysis, and therefore, transmission paths and distribution conditions of the files between hosts in the local area network can be generated. With the method and system adopted, the defect of incapability of performing bidirectional monitoring on file transmission, accurately generating transmission paths and analyzing file distribution in the prior art can be eliminated; a basis can be provided for the backtracking of diffusion conditions of analysis files between the hosts, even if the files are deleted, the historical transmission paths of the files can be still recorded; the distribution conditions of virus files between the hosts can be obtained, and a foundation can be provided for virus elimination, and processing time can be effectively reduced.

The invention discloses a bighead atractylodes rhizome regeneration system for directly differentiating adventitious buds by utilizing hypocotyls and radicles. The bighead atractylodes rhizome regeneration system comprises the following steps: washing and disinfecting bighead atractylodes rhizome seeds; inoculating the disinfected bighead atractylodes rhizome seeds into an MS culture medium; culturing in a dark place until the seeds are germinated; transferring the seeds and continually culturing under light; cutting the radicles and the hypocotyls, cutting into small blocks and taking the small blocks as explants; inoculating the explants into a bud induction culture medium and inducing and culturing; differentiating until green buds grow out; cutting off the green buds and transplanting the green buds into a rooting culture medium for culturing; inducing for rooting; transplanting regenerated plants with roots so as to obtain bighead atractylodes rhizome plants. According to the regeneration system, provided by the invention, the proliferation frequency of bighead atractylodes rhizome is greatly improved, the production cost is saved and the propagation efficiency is improved; technical supports are provided for large-scale production of medical bighead atractylodes rhizome; for the organogenesis which does not subject to calluses, the genetic stability of the plants is higher; quality guarantees are provided for bighead atractylodes rhizome quality improvement, industrial seedling culturing and virus elimination which are realized by utilizing a plant gene engineering technology.

The invention relates to a novel high-efficient quick virus elimination technique for strawberries, which comprises four aspects that: firstly a high-temperature short-time inactivation virus technique is adopted to perform short-time pretreatment about 5 to 20 minutes at the high temperature of 40 to 50 DEG C to realize an effect that partial virus is killed or the virus is inactivated, then the size of meristematic tissues for the best virus elimination effect is determined as 0.2 to 0.5mm under a stereoscopic vision microscope through a meristematic tissue size optimization technique, mitosis and growth of virus-free cell clusters are started through a cell starting culture medium formula, a series of optimization tests for the culture medium are carried out through mass Caespitose shoots culture technique to obtain the best strawberry breeding culture medium formula, and achieved annual production of one million of virus-eliminated strawberries seedlings technical level. By adopting the technique, the virus elimination effect can reach 90 to 100 percent, the virus-eliminated strawberry seedlings are applied and experimented on the production for ten years, the quantity of the application trial points reaches more than 100, and the yield increase effect reaches about 50 percent.

The invention belongs to technical field of oil processing, and concretely relates to a method for removing mycotoxin in the production process of corn oil. The method comprises the following steps: metering of corn germs, cleaning, fragmentation, virus elimination with high temperature and high pressure steam, softening, flaking, pressing and oil production, alkali refining, decolouring, and deodorization; and a finished product corn oil is obtained; wherein the temperature for virus elimination with high temperature and high pressure steam is 160-180 DEG C, the time is 60-80 minutes, and steam pressure is 0.9-1.2MPa. Contents of vomitoxin, aflatoxin B1, Zearalenone and other mycotoxin in the production process of corn oil are less than national limit standards, extra addition of substances or additional installation of large-scale process equipment is not needed, application range is wide without limitation, and the cost is low without potential safety risks of foodstuff; at the same time, new foreign ions are not introduced, so that adverse influences on vitamin E, phytosterol, unsaturated fatty acid and other nutrition components in the corn germs are not generated.

The invention relates to a soilless cultivation method for cultivating miniature seed potatoes through potato virus elimination. The soilless cultivation method includes the step of preparing a seed bed, the step of transplanting tissue culture seedlings, the step of managing rooting of the tissue culture seedlings, the step of preparing nutrient solutions, the step of quantitatively using the different nutrient solutions, the step of controlling the time periods for pouring the nutrient solutions and other steps. The soilless cultivation method is characterized in that the nutrient solutions which are used in soilless cultivation of the seed potatoes are prepared quantitatively, and the times and the time periods of application are set. By means of the soilless cultivation method, the quality, yield and specification of the virus-free miniature seed potatoes are improved, manpower, material resources and financial resources are saved, production cost is reduced, and the ratio of input to output is increased.

The invention relates to an electrochemical air purification technology, and belongs to the field of plasma virus and pathogenic bacteria killing. The invention relates to a method for killing the viruses and the pathogenic bacteria through plasma. Through composite purification steps such as a nano-silver primary filter screen, an active electron capturer, a passive electrostatic adsorber, photoelectrocatalysis and the like, the virus elimination efficiency of 99.999% can be achieved; core disinfection parts of the device adopts electrostatic field disinfection and adsorption, so that the electric energy consumption is greatly reduced, and the replacement frequency of the filter screen is reduced; the core disinfection components such as electrostatic field disinfection and photoelectrocatalysis are non-consumable components, so that the effective service life of the device is effectively prolonged; and in the electrostatic field disinfection and adsorption and photocatalytic disinfection process, the contents of optical ion clusters, charged ions and free radicals are high, an avalanche type chain reaction is effectively induced, the virus disinfection efficiency is improved, ozone molecules generated together are further diffused, and the pathogenic bacteria disinfection efficiency is improved.

The invention discloses a method for cultivating potato virus-free seedlings, relates to the virus elimination and tissue culture techniques of plants, and belongs to the field of agricultural seedling breeding. The method is characterized by comprising the following steps of: transferring virus-free potato tissue culture seedlings into a MS culture medium to culture, wherein a bacteriostatic agent and sugar are added into the MS culture medium; and after the culturing is completed, soaking the seedlings into a CIP improved rooting agent, and carrying out cuttage planting and nutrient solution applying on the seedlings, and after the seedlings are mature, harvesting the seedlings. The method disclosed by the invention has the beneficial effects that through cultivating virus-free seedlings in a special culture medium, the contamination rate in the culture medium is reduced; then, the plant seedlings cultivated in the culture medium are subjected to cuttage, and before cuttage, the cuttage seedlings are placed in the CIP improved rooting agent, and nutrient solutions N, P and K are applied, so that the survival of virus-free seedlings is ensured, the potato growth amount and the expansion of tubers are promoted, thereby improving the yield; and the single-plant potato growth amount of breeder seeds is improved, so that the production cost of breeder seeds is reduced by 25%, thereby providing a reliable technical support for the industrialized development of potatoes.

The invention provides a potato stem tip detoxication method, which comprises the following steps of A1, selecting healthy plants for field marking; selecting big and healthy seed potatoes with standard potato shapes; breaking the dormancy of the tubers of the seed potatoes; then, performing heat treatment for 6 weeks; spraying salicylic acid twice in the period; A2, peeling big sprouts from the potato blocks obtained in the step A1; performing cleaning; A3, under the sterile conditions, cutting 1 to 3mm of the top of the big sprouts; inoculating a liquid culture medium; culturing the big sprouts into test-tube seedlings. The method has the advantages that the stem tip peeling step is simplified; the stem tip peeling technology difficulty is obviously reduced, so that the regenerated plants become strong seedlings from weak seedlings; the conventional multi-step seedling formation is changed into one-step seedling forming; the seedling forming time is about 30 days; the virus elimination period is shortened by about 33.3 percent to 60 percent; the crossing of long period to short period from the potato blocks to virus-free seedlings is realized; the seedling forming rate is increased by 44 percent or more; the current condition of stem tip peeling seedling forming difficulty is changed; the virus elimination rate is improved by 36 percent or higher; the technical bottleneck ofdifficult elimination of PVS and PVM by a conventional potato virus elimination method is broken.

The invention belongs to the technical field of virus elimination of plants, and particularly relates to a virus-elimination method of a plant tissue cultured virus-free explant. The method comprises the following steps: selecting plant mature tissues, performing sterilizing treatment on the selected plant mature tissues, performing primary culture on the sterilized tissues in a primary culture medium, performing propagation culture based on the primary culture, performing virus detection based on the propagation culture, sorting secondary seedlings of which the viruses are negative, extending reproductive output, then performing rooting culture until favorable root systems grow, and then performing domestication and transplanting. Mature tissues are used as explants, so that the tissue culture virus elimination operation is simple, the differentiation rate and the survival rate are greatly increased, and the virus-elimination success rate is high.

The inventive embodiment discloses a virus checking and killing method for video files. The method includes that: video data sections of a video file is read out; when the data format of the video data section is different from that of the video file, the video data section is determined as a virus-infected video data section, and is processed by virus elimination. The inventive embodiment also discloses a virus checking and killing apparatus. The invention can greatly enhance the efficiency of virus checking and killing of the video files and saves system resources.

The invention discloses a high-yielding sowing scheme for allium sativum plants. The scheme comprises the following steps: carrying out virus-free breeding: peeling allium sativum, flushing the peeled allium sativum with water, sterilizing and disinfecting the allium sativum in a disinfectant solution, taking out the disinfected allium sativum, flushing the disinfected allium sativum with sterile water, culturing allium sativum stem tips in a culture medium for 20 days under appropriate conditions, then, carrying out rooting in a propagation culture medium, and then, carrying out culturing for 1.5 months in a nutritive pot, so as to prepare allium sativum; carrying out seed sorting: selecting white, mildew-free and sound allium sativum with the weight of 4.5g to 5.5g as allium sativum seeds; carrying out ploughing: selecting high-quality black land which is fertile and loose in soil texture, ploughing and raking the land, and carrying out fertilizing; and carrying out seeding: furrowing the land, carrying out seeding, applying phoxim granules to the land, covering the seeds with soil, carrying out water irrigation once after 2 to 5 days, and carrying out film covering one day after irrigation. According to the high-yielding sowing scheme for the allium sativum plants, breeding is carried out firstly by adopting a virus elimination technique, and then, seeding is carried out, so that the conditions, i.e., uneven growth and low yield can be effectively improved, and the yield of the allium sativum is increased.

The invention relates to a subway disinfection robot and a control system and method thereof. The subway disinfection robot comprises a chassis walking system, a disinfection spraying and cleaning system and a wireless image transmission system, wherein the chassis walking system comprises a top plate, a bottom plate, a main support, a left side support, a right side support, a bearing pedestal, a driving mechanism, a coupler and Mecanum wheels; the top plate and the bottom plate are fixed through the main support, the left side support and the right side support to form a whole chassis body frame, the bearing seat is installed below the top plate, the driving mechanism is installed above the bottom plate, and the Mecanum wheels are connected to an output end of the driving mechanism. the multiple Mecanum wheels are symmetrically arranged on the two sides of the main support. By automatically spraying the atomized disinfectant, the automatic cleaning device can go deep into tiny gaps which are difficult to touch in ordinary cleaning work, eliminates viruses, bacteria and the like, achieves uniform coverage of sterilization gas, can effectively guarantee the disinfection effect, is high in automation degree, can effectively improve the disinfection efficiency, and reduces labor intensity of cleaning personnel.

The invention provides a network switching method and system based on multi-access edge computing. The method comprises the steps: carrying out the operation state monitoring of a software end of a mobile terminal in an operation state, so as to determine whether each software end is in an abnormal operation state or not; then performing virus checking and killing on interaction data between a software end in an abnormal running state and a mobile network, and determining whether the mobile terminal needs to be switched to be connected to other mobile networks or not according to a data transmission rate of the software end after virus elimination, so that comprehensive real-time monitoring can be performed on the software end on the mobile terminal; then performing virus searching and killing on collected data by utilizing an edge computing strategy, so that the accuracy and comprehensiveness of virus searching and killing are improved to the maximum extent. According to the method, the mobile terminal can be switched to be connected to a mobile network with a larger broadband according to the data transmission rate of a software end; therefore, the mobile terminal is effectively prevented from being continuously attacked by viruses and the communication speed of the mobile terminal is improved.