Edible lily detoxifying fast breeding culture medium

A technology of edible lily and culture medium, applied in the fields of application, plant cells, horticulture, etc., can solve the problems that the detoxification rate is far apart, the detoxification rate is not ideal, etc., and achieve the effect of high survival rate

Inactive Publication Date: 2008-08-06
上海光兆植物速生技术有限公司
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AI Technical Summary

Problems solved by technology

However, the detoxification rate is still not ideal, far from the ideal 100% detoxification rate

Method used

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Embodiment Construction

[0014] A virus-free and rapid propagation medium for edible lily, the start-up medium is MS+6BA 1.0mg / L+NAA 0.1mg / L, and the method of use is:

[0015] Take the bulbs of edible lilies and cultivate them in sand for 7-10 days, peel off the outer scales, peel off the growth points under the dissecting microscope after disinfection, cut out the stem tips with a size of 0.1-0.2mm, inoculate them on the starting medium and cultivate them in the dark. After 3 to 4 days, switch to light culture, the culture temperature is 24±2°C, the light intensity is 2000-3000Lx, and the light time is 12-14 hours / day. The virus-free tissue-cultured seedlings obtained from the micro-shoot tips through the above-mentioned culture have a culture survival rate of 58.9%, and a virus-free rate of 100% in virus detection.

[0016] The virus-free lily tissue culture seedlings were subcultured and strengthened on the subculture medium MS+6BA 0.8mg / L+NAA0.1mg / L, the culture temperature was 24±2°C, the light ...

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Abstract

The invention relates to a virus elimination and rapid propagation medium for edible lily, in particular to a medium having high virus elimination and propagation efficiency aiming at Yixing lily. A virus elimination and rapid propagation medium for edible lily comprises an initial medium, a subculture strong seedling medium, regeneration induction lepisphere, a rooting medium and a bud induction medium, wherein, the initial medium is MS+6BA 0.5-2.5mg / L+NAA 0.1-0.5mg / L. The invention develops the initial medium which has lily stem tip of 0.1-0.2mm survive and improves survival ratio to 58.9 percent; the non virus ratio gets to 100 percent through virus detection; industrial production and large area planting can be carried out by using the subculture strong seedling medium, the regeneration induction lepisphere, the rooting medium and the bud induction medium cooperatively to realize rapid propagation of lily without virus and with high survival ratio.

Description

technical field [0001] The invention relates to a culture medium for detoxification and rapid propagation of edible lily, in particular to a culture medium with extremely high detoxification efficiency and rapid propagation efficiency for Yixing lily. Background technique [0002] Lily virus disease is widely distributed and harmful. It is one of the main diseases in the production of edible lily and fresh-cut flower production, and has been paid attention to all over the world. In recent years, with the rapid increase in the number of lily species introduced and the planting area in my country, as well as the self-propagation and self-use of non-standard bulbs, viral diseases have become prevalent in various lily planting areas in my country, with a general incidence rate of 40-50%. The poisonous rate is more than 90%. After the lily is infected with the virus, it will carry the virus for life and suffer for a long time, destroying the normal physiological functions of the ...

Claims

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Application Information

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IPC IPC(8): A01H4/00C12N5/04
Inventor 宋学孟陈丽张承妹章振华陈明亮
Owner 上海光兆植物速生技术有限公司
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