Potato stem tip detoxication method

Abstract

The invention provides a potato stem tip detoxication method, which comprises the following steps of A1, selecting healthy plants for field marking; selecting big and healthy seed potatoes with standard potato shapes; breaking the dormancy of the tubers of the seed potatoes; then, performing heat treatment for 6 weeks; spraying salicylic acid twice in the period; A2, peeling big sprouts from the potato blocks obtained in the step A1; performing cleaning; A3, under the sterile conditions, cutting 1 to 3mm of the top of the big sprouts; inoculating a liquid culture medium; culturing the big sprouts into test-tube seedlings. The method has the advantages that the stem tip peeling step is simplified; the stem tip peeling technology difficulty is obviously reduced, so that the regenerated plants become strong seedlings from weak seedlings; the conventional multi-step seedling formation is changed into one-step seedling forming; the seedling forming time is about 30 days; the virus elimination period is shortened by about 33.3 percent to 60 percent; the crossing of long period to short period from the potato blocks to virus-free seedlings is realized; the seedling forming rate is increased by 44 percent or more; the current condition of stem tip peeling seedling forming difficulty is changed; the virus elimination rate is improved by 36 percent or higher; the technical bottleneck ofdifficult elimination of PVS and PVM by a conventional potato virus elimination method is broken.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a potato shoot tip detoxification method. Background technique [0002] Potatoes are degraded by virus infection during planting, which directly affects the quality and yield of potatoes, and seriously hinders the production and utilization of potatoes. Potato virus-free technology is the main technical measure to solve the degradation of potato seed. Therefore, virus-free seed potatoes are becoming more and more popular among potato growers. Our country has also made a lot of research results in the tissue culture of potato shoot tip, and established a virus-free seed potato breeding system in various places. Potato shoot tip tissue culture for virus-free seedlings is the foundation of potato virus-free seed potato industry, and high-quality and rapid shoot tip culture wins time and benefits for the production of virus-free seedlings and virus-free potatoes in the future. Establi...

AI Technical Summary

Problems solved by technology

[0007] 3. Virus-free seedlings may mutate. Some hormones will be added to the medium for callus culture after the shoot tip is peeled off. The meristem may be affected by these hormones. Genetic variation may occur, and the potato shoot tip cells are small, and there may be genetic material incomplete