The invention discloses a probe used for EBV detection and a detection method. Two rounds of PCR reaction are needed in the probe preparation process. The detection method based on an NGS technology comprises the following steps: preparing a DNA template, fragmenting genome DNA, purifying a fragmented product, constructing an introduction group library, carrying out PCR amplification on the genomelibrary, hybridizing a probe with the genome library, allowing magnetic beads to capture a hybridization product, carrying out high-throughput sequencing on an obtained captured product, and analyzing the EBV infection condition in a to-be-detected sample. The probe and the method disclosed by the invention have the advantages of low cost, fast data analysis, high coverage depth, high detection accuracy and the like, are applicable to early screening and postoperative diagnosis of diseases caused by EBV, and provide a guidance for personalized medication.