Method for eliminating viruses of pinellia pedatisecta

A technology for removing the palm and leaves of Pinellia sinensis, which is applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of decreased yield and quality, dwarf plants, slow growth, etc., and achieve the effect of improving yield and quality.

Active Publication Date: 2014-10-08
谢晓亮
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In recent years, the artificial planting area of ​​Pinellia palmatum has continued to expand, but the virus infection of Pinellia palmatum is serious, with the diseased area reaching more than 90%. The leaves of infected plants are twisted and covered with chlorotic spots, which are typical symptoms of tobacco mosaic virus; Susceptible plants often appear in high temperature summer, and may also be infected with viroids at the same time. Viroids attack at high temperature and require low temperature treatment to remove
Susceptible plants are dwarfed, grow slowly, or even die, and the yield and quality are greatly reduced

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The method for removing Pinellia palmata virus comprises the following process steps:

[0044] a. Low temperature treatment

[0045] The roots of Pinellia palmatum overwintering under natural conditions shall be covered with 3-5 cm of soil. When the ground temperature at the root is -2°C-3°C, dig out the root soil and take the bud tips that have just germinated;

[0046] b. High temperature treatment

[0047] Rinse the removed bud tips, place them in a petri dish covered with wet filter paper, and place them in an incubator for 15 minutes at a temperature of 50°C at high temperature;

[0048] c. Disinfection treatment

[0049] Rinse the high-temperature-treated bud tips with soapy water and sterilize them on an ultra-clean workbench;

[0050] d, cut bulbil culture:

[0051] Put the sterilized bud tip on the ultra-clean workbench, under the binocular solid dissecting microscope, and dissect the bud tip growth point with a size of 0.3-0.5 mm, inoculate it into the ind...

Embodiment 2

[0074] The difference between this embodiment and embodiment 1 is:

[0075] The length of bud tip in the described step a is 0.3cm.

[0076] The sterilization treatment in the step c is to use alcohol solution to sterilize the bud tip surface for 10 seconds, then sterilize it with 0.1% mercuric chloride solution for 1 minute, rinse it with sterile water for 3 times, and suck the bud body with sterilized filter paper Moisture on the surface in preparation for cutting bulbils.

[0077] The culture conditions in the step d are: temperature 25°C, light 3000Lx.

[0078] All the other contents are the same as in Example 1.

Embodiment 3

[0080] The difference between this embodiment and embodiment 1 is:

[0081] The length of bud tip in the described step a is 0.5cm.

[0082] The sterilization treatment in the step c is to use alcohol solution to sterilize the bud tip surface for 20 seconds, then sterilize it with 0.1% mercuric chloride solution for 3 minutes, wash it with sterile water for 5 times, and suck the bud body with sterile filter paper Moisture on the surface in preparation for cutting bulbils.

[0083] The culture conditions in the step d are: temperature 28°C, light 2000Lx.

[0084] All the other contents are the same as in Example 1.

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Abstract

The invention belongs to a method for eliminating viruses of crops, and in particular relates to a method for eliminating viruses of pinellia pedatisecta. The method comprises the steps of low-temperature treatment, high-temperature treatment, disinfection treatment, bulblet cutting for culture, callus culture or induction, rooting culture, secondary virus elimination, virus detection to obtain virus-free raw seedlings, tissue culture, propagation expansion, acclimatization and transplanting to obtain virus-free pinellia pedatisecta seedlings. The method solves the technical problem of eliminating viruses of the pinellia pedatisecta, can provide the virus-free pinellia pedatisecta seedlings for production, and has the advantages that the principle is utilized appropriately, the method is easy to implement, the large-area pinellia pedatisecta can be effectively planted manually, the yield of the pinellia pedatisecta can be increased, the quality of the pinellia pedatisecta can be further ensured and the like.

Description

technical field [0001] The invention belongs to a method for removing viruses from crops, in particular to a method for removing pinellia palmifolia virus. Background technique [0002] Pinellia pedatisecta Schott is a plant belonging to the genus Pinellia of the family Araceae, also known as Pinellia pedatisecta Schott, a perennial herb. Leaves palmately divided, 9-11 leaflets, petiole slender and weak, light green, 45-65 cm long; spadix terminal, inflorescence stalk equal to or slightly longer than petiole; spathe light green, lanceolate, The lower part is cylindrical, oblong, with a sharp apex, 8-14 cm long: the flowers are unisexual, without perianth, monoecious; the male flowers are born on the upper end of the inflorescence, and the stamens are densely cylindrical, about 6 mm long, [0003] There is a banana aroma; female flowers are born in the lower part of the inflorescence, adnate to the bracts, about 1.5 cm long; the appendages at the apex of the inflorescence ar...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 温春秀谢晓亮刘灵娣贾东升刘铭田伟边建波
Owner 谢晓亮
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