Method for inducing strawberry stolons and eliminating viruses in test tube by high temperature processing in combination with shoot tip culture

Abstract

The invention relates to a method for inducing strawberry stolons and eliminating viruses in a test tube by high temperature processing in combination with shoot tip culture. In the method, the stolons of test-tube plantlets of an excellent new variable variety generated by callus differentiation of deep-mountain strawberry petals are induced in the test tube and the virus elimination of the variety is studied by combining high temperature and shoot tips in the test tube to obtain virus-free strawberry seedlings for production. The method comprises the following steps: inducing the stolons of the obtained new variety in a culture medium in the test tube, wherein the induction rate is above 99.5 percent; culturing the test-tube plantlets containing the stolons for 85d in the test tube at the temperature of 56 DEG C, and cutting 1mm of shoot tip to induce shoot tip calluses in the culture medium; then transferring the shoot tip calluses to the culture medium to perform re-differentiation culture of callus seedlings; and detecting the viruses of the regenerated seedlings, wherein the virus elimination rate is 100 percent. A test result shows that the virus elimination method by high-temperature processing in combination with the shoot tips in the test tube can eliminate the viruses completely so as to establish a strawberry virus elimination system.

Description

[0001] technical field [0002] The invention relates to a plant cultivation method, that is, a method for detoxification of strawberry stolons induced in test tubes combined with high temperature treatment and stem tip cultivation. Background technique [0003] In the prior art, strawberry ( Fragaria chiloensis. Duch.Bail.) is a perennial herb of Rosaceae. In recent years, my country's strawberry production has developed rapidly, and the area and output of strawberry have leapt to the first place in the world. However, because the cultivation of strawberries is through asexual reproduction, after the virus is infected, it spreads along with the asexual propagation materials, so the greater the number of asexual reproduction, the faster the virus transmission speed. After several years of cultivation, it is harmed by the virus. Degradation phenomena such as smaller fruit, poorer quality, and lower yield generally occur, resulting in a reduction in strawberry yield, generall...

AI Technical Summary

Problems solved by technology

Strawberry detoxification research mostly focuses on stem tip detoxification, anther culture detoxification, high temperature treatment and drug treatment, etc., but it is difficult to achieve a 100% detoxification rate. Although the detoxification effect of the stem tip of mm is good, the survival rate is low and complete detoxification cannot be achieved, and it is difficult to peel off the shoot tip, and the operation is easily contaminated, which takes a long time and low work efficiency

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