Chinese jujube micro-grafting method under condition of tissue culture

A micro-grafting and conditional technology, applied in horticultural methods, botanical equipment and methods, plant cells, etc., to achieve the effect of improving screening efficiency

Inactive Publication Date: 2008-08-27
HEBEI AGRICULTURAL UNIV.
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Problems solved by technology

[0005] Through literature review, there is no report on the tissue culture micrografting of jujube

Method used

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  • Chinese jujube micro-grafting method under condition of tissue culture
  • Chinese jujube micro-grafting method under condition of tissue culture

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Embodiment Construction

[0015] The healthy unrooted tissue-cultured seedlings of jujube with jujube were used as rootstock, and the robust tissue-cultured seedlings of the varieties to be tested, Nanjing Damuzao and Bolus jujuba, were used as scions for micro-grafting; the middle stems were selected for both the scion and the rootstock, and the stems of the rootstock were retained. section leaves, and remove the leaves of the scion stem section; cut the rootstock and scion into 2-3cm stem sections, and pass 0.5-1.0mg / L GA 3 Soak in the solution for 10 minutes, then graft by split grafting. After grafting, tie up the grafting joint with tin foil, place the grafted seedlings upright in the culture medium of the culture bottle, and bind the bottle mouth with a single-layer sealing film and cotton plug; the culture medium is MS medium , wherein the sucrose concentration is 50g / L, the pH value of the medium before sterilization is 6.0, and the temperature of the culture room is 28°C for cultivation; taking...

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Abstract

Healthy tissue culture seedlings of a scion variety (strain) are cut into bladeless stem segments of 2-3cm at length to be as scions, healthy tissue culture seedlings of a rootstock variety (strain) are cut into bladed stem segments of 2-3cm at length to be as rootstocks, and the stem segments are soaked and pretreated for 10m in a GA3 solution of 0.5-1.0mg / L in concentration; under an aseptic condition, split grafting is used for carrying out micro-grafting and tinfoil is used for binding grafting interfaces; micro-grafted seedlings are vertically inserted into the culture medium to culture, cotton plugs are used for sealing the bottle mouths of culture bottles and then monolayer plastic sealing membrane is covered and tightly bound; the basic culture medium of the micro-grafted seedlings is MS, wherein, the concentration of the sucrose is 50g / L, the pH value before sterilizing of the culture medium is 6.0 and the temperature of a culture chamber is 28 DEG C. The adopted micro-grafting method can improve the survival rate of the grafting to 83 percent, realize scaled rapid filtration of disease-resistant varieties and idioplasm and is beneficial to develop researches such as the relevant rapid testing of pathogen and virus elimination.

Description

technical field [0001] The invention relates to a jujube micro-grafting method under tissue culture conditions. Background technique [0002] Usually micrografting refers to the technique of grafting rootstock and scion under tissue culture conditions. Since Murashige created micro-bud grafting technology in 1972, micro-grafting technology has been widely used in the research and production of fruit trees such as apples, pears, grapes and citrus, mainly including rapid virus detection, fruit tree rejuvenation, virus-free rapid propagation and Early identification of grafting compatibility and other aspects. [0003] Jujube witches'broom disease is the most common and serious infectious disease in jujube production. Breeding excellent disease-resistant varieties is the fundamental way to solve the problem of jujube witches'broom disease. The jujube madness research group of the China Jujube Research Center of Hebei Agricultural University has selected two highly resistant g...

Claims

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Application Information

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IPC IPC(8): A01G1/06C12N5/04A01H4/00
Inventor 刘孟军代丽赵锦秦子禹刘晓光
Owner HEBEI AGRICULTURAL UNIV.
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